Aedes aegypti exhibits a distinctive mode of late ovarian development

Zhang, Heng; Goh, Feng Guang; Ng, Lee Ching; Chen, Chun Hong; Cai, Yu

doi:10.1186/s12915-023-01511-7

Cited by 11 publications

(9 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The formation of Aedes ovariole, similar to that of Drosophila melanogaster , begins in the late larval stage [ 39 ]. In control pupae, the pre-ovarioles (defined as the immature form of ovariole) formed and resided on the surface of the ovary (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Third, our previous study on Ae. aegypti germline development revealed that the late pupal stage represents the crucial phase for the maturation of ovaries [ 39 ]. These findings establish an optimal window for using irradiation to target the female germline for sterilization.…”

Section: Discussionmentioning

confidence: 99%

“…The tissues were subsequently washed with PBT for 30 min and incubated with DNA staining dye (diluted in PBT) for 1 h. The solution was then removed, and the tissues were stored in the VectaShield mounting medium (Vector Laboratory Inc). The following primary antibodies were used: Guinea pig anti-AaegVasa ([ 39 ], 1:3 k), rabbit anti-cleaved-Caspase 3 (Cell Signaling Technology, #9661,1:200,), mouse anti-H2Av (DSHB, UNC93-5.2.1, 1:1000), mouse anti-PH3 (Abcam, ab14955, 1:10 k). DNA was labelled by TO-PRO-3 (Invitrogen, T3605, 1:5 k) or Hoechst 33,342 (Thermo Fisher Scientific, H21492, 1:5 k).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Different mechanisms of X-ray irradiation-induced male and female sterility in Aedes aegypti

Zhang,

Trueman,

Hou

et al. 2023

BMC Biol

Self Cite

View full text Add to dashboard Cite

Background Aedes aegypti (Ae. aegypti) is the major vector that transmits many diseases including dengue, Zika, and filariasis in tropical and subtropical regions. Due to the growing resistance to chemical-based insecticides, biological control methods have become an emerging direction to control mosquito populations. The sterile insect technique (SIT) deploys high doses of ionizing radiation to sterilize male mosquitoes before the release. The Wolbachia-based population suppression method of the incompatible insect technique (IIT) involves the release of Wolbachia-infected males to sterilize uninfected field females. Due to the lack of perfect sex separation tools, a low percentage of female contamination is detected in the male population. To prevent the unintentional release of these Wolbachia-infected females which might result in population replacement, a low dose of X-ray irradiation is deployed to sterilize any female escapees. However, it remains unclear whether these irradiation-induced male and female sterilizations share common mechanisms. Results In this work, we set out to define the minimum dose of X-ray radiation required for complete female sterilization in Ae. aegypti (NEA-EHI strain). Further results showed that this minimum dose of X-ray irradiation for female sterilization significantly reduced male fertility. Similar results have been reported previously in several operational trials. By addressing the underlying causes of the sterility, our results showed that male sterility is likely due to chromosomal damage in the germ cells induced by irradiation. In contrast, female sterility appears to differ and is likely initiated by the elimination of the somatic supporting cells, which results in the blockage of the ovariole maturation. Building upon these findings, we identified the minimum dose of X-ray irradiation on the Wolbachia-infected NEA-EHI (wAlbB-SG) strain, which is currently being used in the IIT-SIT field trial. Compared to the uninfected parental strain, a lower irradiation dose could fully sterilize wAlbB-SG females. This suggests that Wolbachia-carrying mosquitoes are more sensitive to irradiation, consistent with a previous report showing that a lower irradiation dose fully sterilized Wolbachia-infected Ae. aegypti females (Brazil and Mexican strains) compared to those uninfected controls. Conclusions Our findings thus reveal the distinct mechanisms of ionizing X-ray irradiation-induced male or female sterility in Ae. aegypti mosquitoes, which may help the design of X-ray irradiation-based vector control methods.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Different mechanisms of X-ray irradiation-induced male and female sterility in Aedes aegypti

Zhang,

Trueman,

Hou

et al. 2023

BMC Biol

Self Cite

View full text Add to dashboard Cite

show abstract

“…In Ae. aegypti , follicles in the ovaries containing oocytes are kept in a previtellogenic state until a blood meal is imbibed ( 38 ). Upon blood feeding the ovaries take up proteins and fats from the haemolymph, originating mainly from the fat body, to produce mature oocytes ( 37 , 39 ).…”

Section: Discussionmentioning

confidence: 99%

Protein localization of aquaporins in the adult female disease vector mosquito, Aedes aegypti

Picinic,

Paluzzi,

Donini

2024

Front. Insect Sci.

View full text Add to dashboard Cite

The female Aedes aegypti mosquito is a vector for several arboviral diseases, due to their blood feeding behavior and their association with urban communities. While ion transport in Ae. aegypti has been studied, much less is known about mechanisms of water transport. Rapid water and ion excretion occurs in the adult female mosquito post blood meal and involves a set of organs including the midgut, Malpighian tubules (MTs), and hindgut. The MTs are responsible for the formation of primary urine and are considered the most important site for active transport of ions. Within the cells of the MTs, along with various ion transporters, there are aquaporin water channels that aid in the transport of water across the tubule cell membrane. Six aquaporin genes have been molecularly identified in Ae. aegypti (AQP1–6) and found to be responsible for the transport of water and in some cases, small solutes such as glycerol. In this study, we used immunohistochemistry to localize AaAQP1, 2, 4, 5, and 6 in the adult female Ae. aegypti, in non-blood fed and post blood feeding (0.5 and 24hr) conditions. We further examined the main water transporting aquaporin, AaAQP1, using western blotting to determine protein abundance changes in isolated MTs pre- and post-blood feeding. Using fluorescence in situ hybridization, aqp1 mRNA was found exclusively in the principal cells of female MTs. Finally, we used immunogold staining with transmission electron microscopy to determine subcellular localization of AaAQP1 in the Malpighian tubules under non-blood fed conditions. Interestingly, AaAQP1 was found to be predominantly in the principal cells of the MTs, dispersed throughout the brush border; however, there was also evidence of some AaAQP1 localization in the stellate cells of the MTs.

show abstract

“…The Aedes albopictus (NEA-EHI strain) was obtained from the National Environment Agency (NEA), Singapore. The colony was maintained in the insectary as described previously [ 26 ]. Mosquitoes were infected with CHIKV-BP or CHIKV-SP using a Hemotek membrane feeding system as described earlier [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

Attenuation of neurovirulence of chikungunya virus by a single amino acid mutation in viral E2 envelope protein

Chen,

Phuektes,

Yeo

et al. 2024

J Biomed Sci

Self Cite

View full text Add to dashboard Cite

Background Chikungunya virus (CHIKV) has reemerged as a major public health concern, causing chikungunya fever with increasing cases and neurological complications. Methods In the present study, we investigated a low-passage human isolate of the East/ Central/South African (ECSA) lineage of CHIKV strain LK(EH)CH6708, which exhibited a mix of small and large viral plaques. The small and large plaque variants were isolated and designated as CHIKV-SP and CHIKV-BP, respectively. CHIKV-SP and CHIKV-BP were characterized in vitro and in vivo to compare their virus production and virulence. Additionally, whole viral genome analysis and reverse genetics were employed to identify genomic virulence factors. Results CHIKV-SP demonstrated lower virus production in mammalian cells and attenuated virulence in a murine model. On the other hand, CHIKV-BP induced higher pro-inflammatory cytokine levels, compromised the integrity of the blood–brain barrier, and led to astrocyte infection in mouse brains. Furthermore, the CHIKV-SP variant had limited transmission potential in Aedesalbopictus mosquitoes, likely due to restricted dissemination. Whole viral genome analysis revealed multiple genetic mutations in the CHIKV-SP variant, including a Glycine (G) to Arginine (R) mutation at position 55 in the viral E2 glycoprotein. Reverse genetics experiments confirmed that the E2-G55R mutation alone was sufficient to reduce virus production in vitro and virulence in mice. Conclusions These findings highlight the attenuating effects of the E2-G55R mutation on CHIKV pathogenicity and neurovirulence and emphasize the importance of monitoring this mutation in natural infections.

show abstract

Aedes aegypti exhibits a distinctive mode of late ovarian development

Cited by 11 publications

References 56 publications

Different mechanisms of X-ray irradiation-induced male and female sterility in Aedes aegypti

Different mechanisms of X-ray irradiation-induced male and female sterility in Aedes aegypti

Protein localization of aquaporins in the adult female disease vector mosquito, Aedes aegypti

Attenuation of neurovirulence of chikungunya virus by a single amino acid mutation in viral E2 envelope protein

Contact Info

Product

Resources

About