AEG-1 is involved in hypoxia-induced autophagy and decreases chemosensitivity in T-cell lymphoma

Yan, Jiaqin; Zhang, Junhui; Zhang, Xudong; Li, Xin; Li, Ling; Li, Zhaoming; Chen, Renyin; Zhang, Lei; Wu, Jingjing; Wang, Xinhua; Sun, Zhenchang; Fu, Xiaorui; Chang, Yu; Nan, Feifei; Yu, Hui; Wu, Xiaolong; Feng, Xiaoyan; Li, Wencai; Zhang, Mingzhi

doi:10.1186/s10020-018-0033-6

Cited by 21 publications

(12 citation statements)

References 29 publications

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“…MTdH has been reported to be associated with autophagy. The overexpression of MTdH induces autophagy, while downregulation of MTdH suppresses autophagy (38,39). However, it has also been found that the simultaneous inhibition of autophagy and overexpression of MTdH decreases the levels of P-gp and inhibits 5-Fu resistance (40).…”

Section: Mir-22 Inhibits the Proliferation Of Mg-63 Cells And Mg-63/cmentioning

confidence: 99%

MicroRNA‑22 regulates autophagy and apoptosis in cisplatin resistance of osteosarcoma

et al. 2020

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osteosarcoma (oS) is a primary malignant tumor of bone tissue. effective chemotherapy may improve the survival of patients with OS. MicroRNAs (miRs) serve significant roles in the regulatory function of tumorigenesis and chemosensitivity of different types of cancer. mir-22 has been revealed to inhibit the proliferation and migration of oS cells, as well as increasing their sensitivity to cisplatin (cddP). The mechanisms of action behind the functions of mir-22 in oS drug resistance require investigation. Therefore, in the present study, the human oS cell lines (MG-63, u2oS, Saos2 and oS9901) and a drug-resistant cell line (MG-63/cddP) were cultured. cell proliferation, apoptosis and autophagy assays were performed to investigate the proliferation, apoptosis and autophagy of cell lines transfected with mir-22 mimic. reverse transcription-quantitative polymerase chain reaction and western blot analysis were performed to investigate the expression levels of associated genes. The results revealed that mir-22 inhibited the proliferation of MG-63 cells and MG-63/cddP cells, and enhanced the anti-proliferative ability of cddP. mir-22 induced apoptosis and inhibited autophagy of MG-63 cells and MG-63/cddP cells. apoptosis-related genes, including caspase-3 and Bcl-2-associated X protein were upregulated, while B-cell lymphoma-2 was downregulated in both cell lines transfected with the mir-22 mimic. autophagy protein 5, beclin1 and microtubules-associated protein 1 light chain 3 were downregulated in both cell lines transfected with mir-22 mimic. Furthermore, the in vitro and in vivo expression levels of metadherin (MTdH) in the oS/oS-cddP-resistant models were downregulated following transfection with the mir-22 mimic. Therefore, the results of the present study suggested that mir-22 promoted cddP sensitivity by inhibiting autophagy and inducing apoptosis in oS cells, while MTdH may serve a positive role in inducing cddP resistance of oS cells.

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Section: Mir-22 Inhibits the Proliferation Of Mg-63 Cells And Mg-63/cmentioning

confidence: 99%

MicroRNA‑22 regulates autophagy and apoptosis in cisplatin resistance of osteosarcoma

et al. 2020

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“…In T-cell lymphoma, autophagy was shown to be increased when cells were incubated in hypoxic conditions. Hypoxia-mediated autophagy also clearly decreased the chemosensitivity of lymphoma cells treated with Dox (68).…”

Section: Hypoxia-induced Autophagy In Os Progressionmentioning

confidence: 99%

Insight Into the Role of Autophagy in Osteosarcoma and Its Therapeutic Implication

et al. 2019

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Osteosarcoma is an aggressive bone cancer that frequently metastasizes to the lungs. The cytotoxicity of most chemotherapeutics and targeted drugs in the treatment of osteosarcoma is partially lessened. Furthermore, there is a poor response to current chemo- and radiotherapy for both primary lesions and pulmonary metastases of osteosarcoma. There is a clear need to explore promising drug candidates that could improve the efficacy of osteosarcoma treatment. Autophagy, a dynamic and highly conserved catabolic process, has dual roles in promoting cell survival as well as cell death. The role of autophagy has been investigated extensively in different tumor types, and a growing body of research has highlighted the potential value of using autophagy in clinical therapy. Here, we address significant aspects of autophagy in osteosarcoma, including its functions, modulation, and possible therapeutic applications.

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“…AEG-1 expression was analyzed in lymph node biopsies from 129 T-cell Non-Hodgkin lymphoma (T-NHL) patients and 17 control individuals revealing high AEG-1 expression in T-NHL patients with barely detectable levels in normal lymph nodes ( p < 0.01) [ 130 ]. AEG-1, LC3-II and Beclin-1 levels were found to be significantly higher in T-NHL tissues ( n = 30) versus normal lymph nodes ( n = 16) by Western blot, real-time PCR and IHC ( p < 0.001), suggesting a potential role of AEG-1 in regulating autophagy [ 131 ]. No other clinicopathological parameters were analyzed in these studies.…”

Section: Lymphoma and Leukemia And Aeg-1mentioning

confidence: 99%

The Scope of Astrocyte Elevated Gene-1/Metadherin (AEG-1/MTDH) in Cancer Clinicopathology: A Review

Khan

Sarkar

2021

Genes

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Since its initial cloning in 2002, a plethora of studies in a vast number of cancer indications, has strongly established AEG-1 as a bona fide oncogene. In all types of cancer cells, overexpression and knockdown studies have demonstrated that AEG-1 performs a seminal role in regulating proliferation, invasion, angiogenesis, metastasis and chemoresistance, the defining cancer hallmarks, by a variety of mechanisms, including protein-protein interactions activating diverse oncogenic pathways, RNA-binding promoting translation and regulation of inflammation, lipid metabolism and tumor microenvironment. These findings have been strongly buttressed by demonstration of increased tumorigenesis in tissue-specific AEG-1 transgenic mouse models, and profound resistance of multiple types of cancer development and progression in total and conditional AEG-1 knockout mouse models. Additionally, clinicopathologic correlations of AEG-1 expression in a diverse array of cancers establishing AEG-1 as an independent biomarker for highly aggressive, chemoresistance metastatic disease with poor prognosis have provided a solid foundation to the mechanistic and mouse model studies. In this review a comprehensive analysis of the current and up-to-date literature is provided to delineate the clinical significance of AEG-1 in cancer highlighting the commonality of the findings and the discrepancies and discussing the implications of these observations.

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AEG-1 is involved in hypoxia-induced autophagy and decreases chemosensitivity in T-cell lymphoma

Cited by 21 publications

References 29 publications

MicroRNA‑22 regulates autophagy and apoptosis in cisplatin resistance of osteosarcoma

MicroRNA‑22 regulates autophagy and apoptosis in cisplatin resistance of osteosarcoma

Insight Into the Role of Autophagy in Osteosarcoma and Its Therapeutic Implication

The Scope of Astrocyte Elevated Gene-1/Metadherin (AEG-1/MTDH) in Cancer Clinicopathology: A Review

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