Aegle marmelos fruit extract attenuates Helicobacter pylori Lipopolysaccharide induced oxidative stress in Sprague Dawley rats

Abstract

BackgroundBael (Aegle marmelos (L.) Corr.) has been widely used in indigenous systems of Indian medicine to exploit its medicinal properties including astringent, antidiarrheal, antidysenteric, demulcent, antipyretic, antiulcer, anti-inflammatory and anti cancer activities. The present study aims to evaluate the antioxidative and antiulcer effect of methanolic extract of unripe fruit of Aegle marmelos (MEAM) against Helicobacter pylori-Lipopolysaccharide (HP-LPS) induced gastric ulcer in Sprague Dawley (SD) rats.MethodsDose and duration of HP-LPS and MEAM were fixed based on ulcer index of gastric tissue of experimental animals. Various gastric secretory parameters such as volume of gastric juice, free and total acidity, acid output, pepsin concentration were analyzed. The activities of enzymatic antioxidants (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione transferase), non-enzymatic antioxidants (reduced glutathione, vitamin C and vitamin E) and the levels of lipid peroxidation products were measured. Histological analysis was performed to evaluate the effect of Aegle marmelos on HP-LPS induced gastric ulcer.ResultsOral administration of HP-LPS (50 μg per animal) for four consecutive days resulted in induction of ulcer with the increase in gastric secretory parameters such as volume of gastric juice, free and total acidity, acid output, pepsin concentration. Oral administration of methanolic extract of Aegle marmelos fruit (MEAM) (25, 50, 100, 250 and 500 mg/kg) reduced the gastric ulcer by 2.8 %, 52.4 %, 73 %, 93 % and 93.98 %, respectively, compared to 89.2 % reduction by sucralfate (100 mg/kg). MEAM treatment significantly (p < 0.05) inhibited the increase in gastric secretory parameters in ulcerated rats, and it also prevented the reduction of enzymatic (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione transferase) and non-enzymatic antioxidants (reduced glutathione, vitamin C and vitamin E) after HP-LPS induction. In addition, lipid peroxidation was inhibited by MEAM in HP-LPS induced rats. Results of histological analysis correlated well with biochemical parameters.ConclusionThese observations explored the antioxidant properties of MEAM contributing to the gastroprotective effect in HP-LPS induced gastric ulcer model.

Natural Products for the Prevention and Management of Helicobacter pylori Infection

“…Fruit methanol extract Sprague Dawley rats Prevented reduction of antioxidants Inhibited lipid peroxidation (Ramakrishna et al, 2015).…”

“…Moreover, Achillea millefolium, Berberis aristata, Coriandrum sativum, Foeniculum vulgare, Matricaria chamomilla, and Prunus domestica have been illustrated to significantly inhibit the ROS generation in H. pylori-infected cells (Zaidi et al, 2012). Ramakrishna, Savithri, Kist, & Devaraj (2015) tested the activities of the methanol extract of Aegle marmelos fruit on attenuating H. pylori lipopolysaccharide-induced oxidative stress in Sprague-Dawley rats. It was concluded that the extract ameliorated the gastric ulcer, significantly inhibited the increase of gastric secretory parameters in ulcerated rats, prevented the reduction of enzymatic and nonenzymatic antioxidants after induction, and inhibited lipid peroxidation.…”

Traditional fruits of South India: Bioactive components and their potential health implications in chronic diseases

“…These limitations of current therapy urge for the development of plant‐based therapeutic intervention. It has been reported that A. marmelos fruit extract exerts anti‐ulcerative effect through its antioxidant activity in experimentally induced gastric ulcer model (Ramakrishna, Savithri, Kist, & Devaraj, 2015). Furthermore, the ulcers developed by cold restraint stress in rats and guinea pigs have been effectively treated by pyranocoumarin treatment (Maity, Hansda, Bandyopadhyay, & Mishra, 2009).…”

“…Furthermore, the ulcers developed by cold restraint stress in rats and guinea pigs have been effectively treated by pyranocoumarin treatment (Maity, Hansda, Bandyopadhyay, & Mishra, 2009). Studies have shown that rats pretreated with immature A. marmelos fruit extract reduced gastric ulcer induced by absolute ethanol (Ramakrishna et al., 2015).…”

Exploring antibacterial & antiulcer activity of aegle marmelos linn. : A review

“…Oral administration of methanolic extract of Aegle marmelos fruit reduced the gastric ulcer compared to standard drug sucralfate and significantly inhibited the increase in gastric secretory parameters in ulcerated rats It was concluded that antioxidant properties of Aegle marmelos contributes to the gastroprotective effect in Helicobacter pylori-Lipopolysaccharide induced gastric ulcer model. 39 G. Vimala & F. Gricilda Shoba (2014) discussed some medicinal plants which are mentioned in Ayurveda and also in modern science for the treatment or prevention of peptic ulcer. 8 Pushpendra K. Patel et al (2012) summarizes the pharmacological activity of Aegle marmelos based on scientific information.…”