Aeluropus littoralis NaCl-induced vacuolar H+-ATPase Subunit c: Molecular cloning and expression analysis

Nasiri, Najmeh; Shokri, Ehsan; Nematzadeh, Ghorbanali

doi:10.1134/s1022795412080054

Cited by 4 publications

(4 citation statements)

References 29 publications

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“…Its subunit genes were upregulated under the action of antineoplastic agents [30,31]. Therefore, we speculated that V-ATPase was related to the drug resistance in tumor tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Although this study did not prove that the expression of V-ATPase was directly linked to the drug resistance of cancer tissues, other experiments have already shown that the activity of V-ATPase was increased in multidrug-resistant cell lines. Its subunit genes were upregulated under the action of antineoplastic agents [ 30 , 31 ]. Therefore, we speculated that V-ATPase was related to the drug resistance in tumor tissues.…”

Section: Discussionmentioning

confidence: 99%

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The expression of V-ATPase is associated with drug resistance and pathology of non-small-cell lung cancer

Lü

Huang

et al. 2013

Diagn Pathol

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ObjectiveThis article aims to investigate the expression of vacuolar-H + −ATPase (V-ATPase) in non-small cell lung cancer (NSCLC) and its variations with pathological type and grade. Furthermore, to evaluate the chemotherapy drug sensitivity of different cancer tissues as well as its correlation with V-ATPase expression in NSCLC.MethodsV-ATPase expression was examined in 92 NSCLC tissue samples using the immunohistochemical Envision method and immunofluorescence assay. The location of V-ATPase expression was observed by confocal laser scanning microscopy and the difference of its expression rate was evaluated. The sensitivity of cancer tissues to chemotherapy drug was examined using MTT assay and its correlation with the V-ATPase expression was tested in NSCLC by Spearman rank correlation analysis.ResultsV-ATPase expression was mainly localized in the cell membrane and cytoplasm. The expression rate of V-ATPase was 71.43% in squamous cell lung cancer, significantly lower than that of the lung adenocarcinoma (83.72%, P = 0.000). In different pathological grades of squamous cell lung cancer, the expression rate of V-ATPase was 58.33% in grade II, significantly lower than that of the grade III (84.00%, P = 0.014). The expression rate of V-ATPase in grade II lung adenocarcinoma was 76.67%, significantly lower than that of the grade ΙΙΙ adenocarcinoma (100.0%, P = 0.012). Correlation analysis showed that the sensitivity of NSCLC tissues to cyclophosphamide, gemcitabine, doxorubicin, paclitaxel and cisplatin was significantly correlated with the V-ATPase expression rate (P < 0.05).ConclusionsV-ATPase was overexpressed in NSCLC. The expression of V-ATPase was related to the pathological type and grade of cancer and was likely associated with chemotherapy drug resistance in NSCLC.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/7515811511020000

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“…Its subunit genes were upregulated under the action of antineoplastic agents [30,31]. Therefore, we speculated that V-ATPase was related to the drug resistance in tumor tissues.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The expression of V-ATPase is associated with drug resistance and pathology of non-small-cell lung cancer

Lü

Huang

et al. 2013

Diagn Pathol

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“…Therefore, we have modified the original HS method and developed a new protocol to extract intact chloroplasts and prepare pure cpDNA from the halophyte grass Aeluropus littoralis (Gouan) Parl. Aeluropus littoralis, which is considered to be a salt-tolerant plant, is an Iranian endemic monocot halophyte with notable biological characteristics, including the evolution of a series of mechanisms to maintain osmotic balance and enhance salt tolerance (Nasiri et al, 2012). We also tested our method on other Poaceae species and confirmed that it is effective in preparing cpDNA of high quality.…”

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confidence: 67%

NaOH low‐salt method for chloroplast isolation and highly pure cpDNA preparation from Aeluropus littoralis

et al. 2018

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Premise of the StudyHigh‐yield pure chloroplast DNA (cpDNA) is necessary for whole genome sequencing. Chloroplast extraction with traditional high‐salt methods causes damage to nuclei and destroys the integrity of organelles, which leads to high genomic contamination from the nucleus and mitochondria. To overcome this issue, we modified a traditional high‐salt method to obtain a new approach called the NaOH low‐salt method (NLS).Methods and ResultsThe NLS method is based on the mild alkaline lysis of plant cells, followed by homogenization with ultrasonic waves and fractionation under reduced osmotic pressure. Results showed that this modified protocol worked efficiently to extract the intact chloroplast from Aeluropus littoralis and other grasses to obtain high‐quality pure cpDNA, which was confirmed by fluorescent microscopy, qPCR, and Illumina paired‐end sequencing analysis.ConclusionsCompared with high‐salt methods, the NLS method has proven robust for extraction of intact chloroplasts and preparation of high‐yield pure cpDNA from grasses.

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“…V-ATPase activity is adjusted mostly by protein expression. Post translational modifications play a minor role under salinity, though some are known ( Wang et al, 2001 ; Nasiri et al, 2012 ; Cosentino et al, 2013 ). Polyamines decrease proton pumping activity in cucumber, so salinity-dependent activation of proton pumps is accompanied by decrease in polyamines; this might occur by transamidation of proteins, since high expression levels of transglutamidases result in higher activity of V-PPase and V-ATPase ( Janicka-Russak et al, 2010 ; Zhong et al, 2020 ).…”

Section: V-atpase and Abiotic Stress Response: Some Examplesmentioning

confidence: 99%

The Plant V-ATPase

Seidel

2022

Front. Plant Sci.

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V-ATPase is the dominant proton pump in plant cells. It contributes to cytosolic pH homeostasis and energizes transport processes across endomembranes of the secretory pathway. Its localization in the trans Golgi network/early endosomes is essential for vesicle transport, for instance for the delivery of cell wall components. Furthermore, it is crucial for response to abiotic and biotic stresses. The V-ATPase’s rather complex structure and multiple subunit isoforms enable high structural flexibility with respect to requirements for different organs, developmental stages, and organelles. This complexity further demands a sophisticated assembly machinery and transport routes in cells, a process that is still not fully understood. Regulation of V-ATPase is a target of phosphorylation and redox-modifications but also involves interactions with regulatory proteins like 14-3-3 proteins and the lipid environment. Regulation by reversible assembly, as reported for yeast and the mammalian enzyme, has not be proven in plants but seems to be absent in autotrophic cells. Addressing the regulation of V-ATPase is a promising approach to adjust its activity for improved stress resistance or higher crop yield.

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Aeluropus littoralis NaCl-induced vacuolar H+-ATPase Subunit c: Molecular cloning and expression analysis

Cited by 4 publications

References 29 publications

The expression of V-ATPase is associated with drug resistance and pathology of non-small-cell lung cancer

The expression of V-ATPase is associated with drug resistance and pathology of non-small-cell lung cancer

NaOH low‐salt method for chloroplast isolation and highly pure cpDNA preparation from Aeluropus littoralis

The Plant V-ATPase

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