Aeration and Glucose Concentration on Saccharomyces boulardii Probiotic Cultivation

Abstract

Saccharomyces boulardii viable cells have probiotic action. This study aimed to evaluate the effect of aeration on the production of S. boulardii viable cells in liquid cultivation medium with different initial glucose concentrations. Yeast has grown on yeast extract peptone dextrose medium with or without aeration (2 Lar min-1 to obtain 30 ± 1% of oxygen) and different initial glucose concentrations (20, 40 and 60 g L-1). The viable cells were determined by serial dilution method and glucose concentration was determined by 3-5 dinitrosalicylic acid. The number of viable cells changed from 7.54 ± 0.04 to 7.77 ± 0.02 log of CFU mL-1 when glucose increased from 20 to 60 g L-1, respectively. The aeration of the cultivation medium increased the number of viable cells from 7.78 ± 0.04 to 8.27 ± 0.04 log of CFU mL-1 in the cultivation medium with 40 g L-1 glucose. The yeast biomass was ~3 times greater than 60 g L-1 glucose with aeration when compared to cultivation medium with 20 g L-1 glucose without aeration. Thus, the glucose increase and the cultivation medium aeration increment S. boulardii production and viability.