Aerial Parts of Peucedanum chenur Have Anti-Cancer Properties through the Induction of Apoptosis and Inhibition of Invasion in Human Colorectal Cancer Cells

Abstract

Background: The Peucedanum species have many pharmacological effects due to the presence of coumarins, flavonoids, phenolic compounds, and essential fatty acids in these species. In this study, for the first time, the anticancer activity of Peucedanum chenur methanolic extract via the induction of apoptosis and inhibition of invasion in HCT-116 human colon cancer cells was investigated. Methods: P. chenur methanolic extract effect on HCT-116 cells viability and antioxidant activity were evaluated using MTT assay, DPPH, and iron chelating tests, respectively. Changes in mRNA expression level in a panel of relevant genes were assessed by the quantitative real-time PCR. Also, apoptosis was assessed by cell cycle analysis and Annexin V/PI method, and the effect on cell migration was tested using scratch test. Results: P. chenur methanolic extract increased significantly the expression of BAX while decreased the expression of BCL-2, AKT1, FAK, RhoA, and MMP genes compared to the control group. BAX/BCL-2 ratio and apoptosis elevated, whereas cell migration reduced significantly. Besides, our extract showed an appropriate antioxidant activity. Conclusion: P. chenur may be introduced as a new chemopreventive agent in medicine due to its notable power in terms of induction of apoptosis and inhibition of invasion.

Integrative analysis of chloroplast genome, chemicals, and illustrations in Bencao literature provides insights into the medicinal value of Peucedanum huangshanense

“…For instance, the leaf and rhizome of P. ostruthium (L.) W. D. J. Koch were developed to treat Alzheimer's disease and dermatosis in central and southern Europe (Palmioli et al, 2019;Danna et al, 2022). In addition, P. chenur Have is an endemic species of Kurdistan in western Iran, aerial parts of P. chenur has inhibitory effect on human colon cancer cells (Yadegari et al, 2020). Moreover, the volatile oils in the fruits of P. dhana A. Ham have anti-inflammatory and antioxidant activities as well as cytotoxic effects on cancer cells; they are used to treat colon adenocarcinoma in Thailand (Khruengsai et al, 2021).…”