Aerobactin-mediated iron uptake by Escherichia coli isolates from human extraintestinal infections

Carbonetti, Nicholas H.; Boonchai, S; Parry, S H; Väisänen‐Rhen, Vuokko; Korhonen, T K; Williams, Paul H.

doi:10.1128/iai.51.3.966-968.1986

Cited by 136 publications

(47 citation statements)

References 19 publications

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“…The number of E + isolates was higher than that of AEisolates, and the number of A + isolates was higher than that of E + isolates, but neither difference reached statistical significance ( Table 2). The presence of siderophores and other virulence factors in Gram-negative bacteria has been investigated previously [11][12][13][14]. In the present study, the proportion of aerobactin-producing E. coli isolates was significantly higher in the UTI and extra-urinary samples, which is consistent with the results of other studies [14,15].…”

Section: Introductionsupporting

confidence: 92%

“…The presence of siderophores and other virulence factors in Gram-negative bacteria has been investigated previously [11][12][13][14]. In the present study, the proportion of aerobactin-producing E. coli isolates was significantly higher in the UTI and extra-urinary samples, which is consistent with the results of other studies [14,15]. Ruiz et al [2] found that production of aerobactin in E. coli isolates from patients with nephritis was higher than in isolates from patients with cystitis.…”

Section: Introductionsupporting

confidence: 90%

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Production by Escherichia coli isolates of siderophore and other virulence factors and their pathogenic role in a cutaneous infection model

Demir

Kaleli

2004

Clinical Microbiology and Infection

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Escherichia coli isolates from urinary tract infections (UTIs) (n = 124), extra-urinary sites (n = 37) and normal faecal samples (n = 51) were examined for the presence of virulence factors, including siderophores (aerobactin and enterobactin). The proportion of aerobactin producers was significantly higher in UTI (69.4%; p 0.001) and extra-urinary samples (70.3%; p 0.007) than in controls (41.2%), while the proportion of enterobactin producers was significantly lower in the UTI samples than in the controls (p 0.027). In a cutaneous infection model, aerobactin-positive E. coli showed more growth than non-aerobactin and non-enterobactin isolates, even when other virulence factors were identical.

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Section: Introductionsupporting

confidence: 92%

Section: Introductionsupporting

confidence: 90%

Production by Escherichia coli isolates of siderophore and other virulence factors and their pathogenic role in a cutaneous infection model

Demir

Kaleli

2004

Clinical Microbiology and Infection

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“…The iutA gene was detected in 12 (26.7%) of the 45 EAEC strains. Production of this iron utilization system is significantly greater among E. coli isolates from patients with septicaemia, pyelonephritis and lower urinary tract infections than among faecal isolates of healthly controls [17].…”

Section: Distribution Of Genes Encoding Iron Acquisition Systems Amomentioning

confidence: 99%

Distribution of genes encoding iron uptake systems among enteroaggregative Escherichia coli strains isolated from adults with irritable bowel syndrome

Sobieszczańska¹

2008

Clinical Microbiology and Infection

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The distribution of genes encoding different iron acquisition systems in enteroaggregative Escherichia coli (EAEC) from adults with irritable bowel syndrome and from healthy controls was examined using a PCR assay. As many as 95.5% of EAEC carried the chuA gene coding for a haem receptor, and the majority of these strains also had yersiniobactin-encoding genes. Apart from yersiniobactin, enterobactin was the siderophore most frequently associated with EAEC among those strains examined. Genes encoding aerobactin and salmochelin siderophores were less frequent in the group of EAEC.

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“…Strain C1845 and some other DAEC strains were present in the cluster DAEC1 and appear to be phylogenetically close to the EAEC strains. Moreover, Afa/Dr DAEC strains appear generally to express several characteristics that have been associated with extraintestinal E. coli strains, including the B2 phylogenetic group (188,341), the O75 serotype (312), the production of aerobactin (71,130,441), the presence of iroN (358), and the presence of sequences from PAI CFT073 (168), but not including the hlyA, hlyD, hp1 to hp4, papG, or papF sequences. As an exception, the pyelonephritogenic Afa/Dr DAEC strain EC7372, which harbors the Dr-II adhesin (340), expresses a functional hemolysin that is responsible for cell death by apoptosis or necrosis (165).…”

Section: Introductionmentioning

confidence: 99%

Pathogenesis of Afa/Dr Diffusely AdheringEscherichia coli

2005

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Over the last few years, dramatic increases in our knowledge about diffusely adhering Escherichia coli (DAEC) pathogenesis have taken place. The typical class of DAEC includes E. coli strains harboring AfaE-I, AfaE-II, AfaE-III, AfaE-V, Dr, Dr-II, F1845, and NFA-I adhesins (Afa/Dr DAEC); these strains (i) have an identical genetic organization and (ii) allow binding to human decay-accelerating factor (DAF) (Afa/DrDAF subclass) or carcinoembryonic antigen (CEA) (Afa/DrCEA subclass). The atypical class of DAEC includes two subclasses of strains; the atypical subclass 1 includes E. coli strains that express AfaE-VII, AfaE-VIII, AAF-I, AAF-II, and AAF-III adhesins, which (i) have an identical genetic organization and (ii) do not bind to human DAF, and the atypical subclass 2 includes E. coli strains that harbor Afa/Dr adhesins or others adhesins promoting diffuse adhesion, together with pathogenicity islands such as the LEE pathogenicity island (DA-EPEC). In this review, the focus is on Afa/Dr DAEC strains that have been found to be associated with urinary tract infections and with enteric infection. The review aims to provide a broad overview and update of the virulence aspects of these intriguing pathogens. Epidemiological studies, diagnostic techniques, characteristic molecular features of Afa/Dr operons, and the respective role of Afa/Dr adhesins and invasins in pathogenesis are described. Following the recognition of membrane-bound receptors, including type IV collagen, DAF, CEACAM1, CEA, and CEACAM6, by Afa/Dr adhesins, activation of signal transduction pathways leads to structural and functional injuries at brush border and junctional domains and to proinflammatory responses in polarized intestinal cells. In addition, uropathogenic Afa/Dr DAEC strains, following recognition of β1 integrin as a receptor, enter epithelial cells by a zipper-like, raft- and microtubule-dependent mechanism. Finally, the presence of other, unknown virulence factors and the way that an Afa/Dr DAEC strain emerges from the human intestinal microbiota as a “silent pathogen” are discussed

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Aerobactin-mediated iron uptake by Escherichia coli isolates from human extraintestinal infections

Cited by 136 publications

References 19 publications

Production by Escherichia coli isolates of siderophore and other virulence factors and their pathogenic role in a cutaneous infection model

Production by Escherichia coli isolates of siderophore and other virulence factors and their pathogenic role in a cutaneous infection model

Distribution of genes encoding iron uptake systems among enteroaggregative Escherichia coli strains isolated from adults with irritable bowel syndrome

Pathogenesis of Afa/Dr Diffusely AdheringEscherichia coli

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