Aerobactin-mediated iron uptake system in intestinal Escherichia coli

Elena-Fernandez-Beros, M

doi:10.1016/0378-1097(88)90332-1

Cited by 3 publications

(9 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further work is required to determine the distribution of Klebsiella strains expressing a functional aerobactin receptor without apparent aerobactin production. A high incidence of the aerobactin receptor in the absence of aerobactin production has been observed in E. coli strains isolated from children with diarrhoea (Fernandez-Beros et al, 1988). In these strains the receptor was located either on a plasmid or in the chromosome but in contrast to the klebsiellae described here, all cloacinsensitive strains hybridized with the pColV-K30 aerobactin gene probe (Fernandez-Beros et al, 1988).…”

Section: Discussionmentioning

confidence: 82%

“…Since the first indications that the aerobactin iron-uptake system was associated with virulence, considerable efforts have been directed towards elucidating its distribution and genetic organization within virulent enteroinvasive, enteropathogenic and enterotoxigenic strains of E. coli (Griffiths, 1987;Roberts et al, 1986;Carbonetti et al, 1986;Fernandez-Beros et al, 1988;Marolda et al, 1987). This work has been extended to other enterobacterial pathogens including the Klebsiellae (Nassif & Sansonetti, 1986;Williams et al, 1987).…”

Section: Discussionmentioning

confidence: 96%

“…A high incidence of the aerobactin receptor in the absence of aerobactin production has been observed in E. coli strains isolated from children with diarrhoea (Fernandez-Beros et al, 1988). In these strains the receptor was located either on a plasmid or in the chromosome but in contrast to the klebsiellae described here, all cloacinsensitive strains hybridized with the pColV-K30 aerobactin gene probe (Fernandez-Beros et al, 1988). In another study of some 500 strains of E. coli from human extraintestinal infections, Carbonetti et al (1986) reported that around 50% of the strains examined expressed the aerobactin iron-uptake system.…”

Section: Discussionmentioning

confidence: 98%

See 2 more Smart Citations

Novel Aerobactin Receptor in Klebsiella pneumoniae

Williams¹,

Smith²,

Stevenson³

et al. 1989

Microbiology

View full text Add to dashboard Cite

Several Klebsiella pneumoniae strains which produced enterochelin but not aerobactin were nevertheless sensitive to cloacin DF13. In contrast, a strain of serotype K1:O1 which produced both siderophores was cloacin-resistant. Loss by mutation of the O1 but not K1 antigen rendered this strain cloacin-sensitive, indicating that the O1 antigen prevented access of cloacin to the cloacin/aerobactin receptor. Unlike the K1:O1 strain, the aerobactin-negative strains failed to hybridize in a colony blot assay with an aerobactin receptor gene probe prepared from pColV-K30. However, antisera raised against the 74 kDa pColV-K30 aerobactin receptor cross-reacted with a 76 kDa outer-membrane protein in each K. pneumoniae strain. In addition to the 76 kDa protein, the K1:O1 strain also produced a strongly cross-reacting 74 kDa protein. To determine whether these aerobactin-negative strains could use aerobactin, mutants unable to synthesize siderophores were isolated. Aerobactin promoted the growth of these mutants in iron-deficient media. The evidence presented suggests that some K. pneumoniae strains produce an aerobactin iron-uptake system without apparent production of aerobactin and which is probably based on a 76 kDa receptor, the gene for which does not hybridize with aerobactin receptor gene encoded on pColV-K30.

show abstract

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Novel Aerobactin Receptor in Klebsiella pneumoniae

Williams¹,

Smith²,

Stevenson³

et al. 1989

Microbiology

View full text Add to dashboard Cite

show abstract

“…Of the 18 blood isolates containing ColV plasmids, 11 were chosen for further study. It was found that each of the ColV plasmids hybridized with the traT, iss, and iu DNA probes.…”

Section: Resultsmentioning

confidence: 99%

“…1984 [11]). The 100 strains isolated in 1982 to 1983 included 17 enterotoxigenic E. coli strains, 2 enteropathogenic E. coli strains, and one strain that produced Vero toxin.…”

Section: Methodsmentioning

confidence: 99%

Virulence-related genes in ColV plasmids of Escherichia coli isolated from human blood and intestines

et al. 1990

View full text Add to dashboard Cite

DNA probes for the colicin V, traT, iss, and iu genes were used in this study of four representative ColV plasmids together with 200 Escherichia coli strains isolated from the stools of patients with diarrhea and 146 E. coli strains isolated from the blood of patients with bacteremia. The study indicated that the ColV plasmids are heterogeneous. Southern and colony hybridization analyses showed that in most of the colicin V-producing intestinal E. coli strains, the colicin V genes are located in the chromosome (14 of 16); in most of the colicin V-producing E. coli strains isolated from the blood, they are located in plasmids (18 of 22). In both intestinal and blood E. coli isolates, the traT, iss, and aerobactin receptor genes were present at similar frequencies, but the frequency of the aerobactin synthesis genes was significantly different. The aerobactin receptor gene was present in 25% of the intestinal E. coli strains that lack the aerobactin synthesis gene. In the blood isolates, the aerobactin synthesis and receptor genes were present at almost equal frequencies. Among the colicin V-producing isolates, the iss, traT, and iu genes were present in 95.5, 86.4, and 90.9% of the blood isolates and in only 68.8, 43.8, and 81.3% of the intestinal isolates, respectively. The ColV plasmids from blood isolates that were tested for the presence of traT, iss, and iu genes were homogeneous and had DNA sequences that hybridized with each of the probes. On the other hand, the two intestinal strains containing ColV genes in a plasmid were heterogeneous in regard to the carriage of these genes. The presence of ColV is not restricted to specific O types.

show abstract

Chromosomal DNA, iron-transport systems, outer membrane proteins, and enterotoxin (heat labile) production in Salmonella typhi strains

1990

View full text Add to dashboard Cite

We examined a representative collection of Salmonella typhi strains from Chile, Peru, Mexico, India, and England for the presence of several properties. Ail strains had a conserved pattern of outer membrane proteins, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The electrophoresis profiles of chromosomal DNA digested with EcoRI and PstI restriction enzymes were similar for all the strains. A conserved pattern of hybridization was observed when digested chromosomal DNA was hybridized with DNA probes for the 36-kilodalton porin, enterobactin synthesis, and enterobactin receptor genes. AUl the strains produced enterobactin but not aerobactin in bioassays. None of the strains produced heat-labile toxin, as measured by an enzyme-linked immunosorbent assay. Colony and Southern hybridizations with DNA probes for aerobactin synthesis and its receptor and heat-labile toxin genes were negative. These results indicate that S. typhi strains from different origins have similar phenotypic and genetic properties and, as has been suggested, constitute a clone.

show abstract

Aerobactin-mediated iron uptake system in intestinal Escherichia coli

Cited by 3 publications

References 4 publications

Novel Aerobactin Receptor in Klebsiella pneumoniae

Novel Aerobactin Receptor in Klebsiella pneumoniae

Virulence-related genes in ColV plasmids of Escherichia coli isolated from human blood and intestines

Chromosomal DNA, iron-transport systems, outer membrane proteins, and enterotoxin (heat labile) production in Salmonella typhi strains

Contact Info

Product

Resources

About