Aerobic and nitrite-dependent methane-oxidizing microorganisms in sediments of freshwater lakes on the Yunnan Plateau

Liu, Yong; Zhang, Jingxu; Zhao, Liang; Li, Yuzhao; Yang, Yuyin; Xie, Shuguang

doi:10.1007/s00253-014-6141-5

Cited by 54 publications

(20 citation statements)

References 41 publications

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“…The first three were most closely related to uncultured species from other freshwater lakes, reservoirs, and sediments: Lake Mizugaki (Kojima et al, 2009; Tsutsumi et al, 2011), Feitsui Reservoir (Kojima et al, 2014), Lake Cadagno (Milucka et al, 2015), Lake Kinneret (Junier et al, 2010), Lake Schoehsee (unpublished), Lake Constance (Pester et al, 2004), and lakes on the Yunnan Plateau (Liu et al, 2015). The fourth cluster was closely related to an uncultured Methylococcus species from a wetland in northeast China (Yun et al, 2013) and an uncultured bacterium from lake sediments (Liu et al, 2015). Sequenced clones belonging to type II (alpha-) MOB were most closely related to cultured and uncultured species of Methylocystis .…”

Section: Resultsmentioning

confidence: 99%

Methanotrophy under Versatile Conditions in the Water Column of the Ferruginous Meromictic Lake La Cruz (Spain)

et al. 2016

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Lakes represent a considerable natural source of methane to the atmosphere compared to their small global surface area. Methanotrophs in sediments and in the water column largely control methane fluxes from these systems, yet the diversity, electron accepting capacity, and nutrient requirements of these microorganisms have only been partially identified. Here, we investigated the role of electron acceptors alternative to oxygen and sulfate in microbial methane oxidation at the oxycline and in anoxic waters of the ferruginous meromictic Lake La Cruz, Spain. Active methane turnover in a zone extending well below the oxycline was evidenced by stable carbon isotope-based rate measurements. We observed a strong methane oxidation potential throughout the anoxic water column, which did not vary substantially from that at the oxic/anoxic interface. Both in the redox-transition and anoxic zones, only aerobic methane-oxidizing bacteria (MOB) were detected by fluorescence in situ hybridization and sequencing techniques, suggesting a close coupling of cryptic photosynthetic oxygen production and aerobic methane turnover. Additions of nitrate, nitrite and to a lesser degree iron and manganese oxides also stimulated bacterial methane consumption. We could not confirm a direct link between the reduction of these compounds and methane oxidation and we cannot exclude the contribution of unknown anaerobic methanotrophs. Nevertheless, our findings from Lake La Cruz support recent laboratory evidence that aerobic methanotrophs may be able to utilize alternative terminal electron acceptors under oxygen limitation.

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Section: Resultsmentioning

confidence: 99%

Methanotrophy under Versatile Conditions in the Water Column of the Ferruginous Meromictic Lake La Cruz (Spain)

et al. 2016

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“…The sediment cores were transported on ice to the laboratory for analysis. These sediment samples had been used for the molecular analysis of methaneoxidizing populations in our previous study (Liu et al 2014c). The geographic and limnological parameters of these 13 plateau lakes, location of sampling sites, and the chemical properties of the collected lake sediment samples were described in detail in the literature (Liu et al 2014c).…”

Section: Study Sites and Sediment Samplesmentioning

confidence: 99%

“…The Yunnan Plateau (southwestern China), located in the subtropical or temperate climate zone, has about 40 freshwater lakes with an area of larger than 1 km 2 . These lakes are distributed in different geographical regions and relatively separated (Liu et al 2014c). Therefore, the objective of the present study was to investigate the sediment bacterial and archaeal communities in a number of freshwater lakes on the Yunnan Plateau.…”

Section: Introductionmentioning

confidence: 99%

Distribution of sediment bacterial and archaeal communities in plateau freshwater lakes

Zhang

Yang

Zhao³

et al. 2014

Appl Microbiol Biotechnol

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243

105

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Both Bacteria and Archaea might be involved in various biogeochemical processes in lacustrine sediment ecosystems. However, the factors governing the intra-lake distribution of sediment bacterial and archaeal communities in various freshwater lakes remain unclear. The present study investigated the sediment bacterial and archaeal communities in 13 freshwater lakes on the Yunnan Plateau. Quantitative PCR assay showed a large variation in bacterial and archaeal abundances. Illumina MiSeq sequencing illustrated high bacterial and archaeal diversities. Bacterial abundance was regulated by sediment total organic carbon and total nitrogen, and water depth, while nitrate nitrogen was an important determinant of bacterial diversity. Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Chlorobi, Chloroflexi, Cyanobacteria, Firmicutes, Gemmatimonadetes, Nitrospirae, Planctomycetes, and Verrucomicrobia were the major components of sediment bacterial communities. Proteobacteria was the largest phylum, but its major classes and their proportions varied greatly among different lakes, affected by sediment nitrate nitrogen. In addition, both Euryarchaeota and Crenarchaeota were important members in sediment archaeal communities, while unclassified Archaea usually showed the dominance.

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“…Archaea is found having an important role in global biogeochemical processes, such as methanogenesis and methane oxidation [2], sulphate reduction [3], and ammonia oxidation [4, 5]. In freshwater environments, active archaea community is responsible for methane release and nitrogen transformation, especially in benthonic water and sediments [6]. It is supposed that lacustrine ecosystems contribute nearly 6–16% of the total natural methane emission on a global scale [7].…”

Section: Introductionmentioning

confidence: 99%

The Distribution Pattern of Sediment Archaea Community of the Poyang Lake, the Largest Freshwater Lake in China

Liu

Kong

et al. 2016

Archaea

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Archaea plays an important role in the global geobiochemical circulation of various environments. However, much less is known about the ecological role of archaea in freshwater lake sediments. Thus, investigating the structure and diversity of archaea community is vital to understand the metabolic processes in freshwater lake ecosystems. In this study, sediment physicochemical properties were combined with the results from 16S rRNA clone library-sequencing to examine the sediment archaea diversity and the environmental factors driving the sediment archaea community structures. Seven sites were chosen from Poyang Lake, including two sites from the main lake body and five sites from the inflow river estuaries. Our results revealed high diverse archaea community in the sediment of Poyang Lake, including Bathyarchaeota (45.5%), Euryarchaeota (43.1%), Woesearchaeota (3.6%), Pacearchaeota (1.7%), Thaumarchaeota (1.4%), suspended Lokiarchaeota (0.7%), Aigarchaeota (0.2%), and Unclassified Archaea (3.8%). The archaea community compositions differed among sites, and sediment property had considerable influence on archaea community structures and distribution, especially total organic carbon (TOC) and metal lead (Pb) (p < 0.05). This study provides primary profile of sediment archaea distribution in freshwater lakes and helps to deepen our understanding of lake sediment microbes.

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Aerobic and nitrite-dependent methane-oxidizing microorganisms in sediments of freshwater lakes on the Yunnan Plateau

Cited by 54 publications

References 41 publications

Methanotrophy under Versatile Conditions in the Water Column of the Ferruginous Meromictic Lake La Cruz (Spain)

Methanotrophy under Versatile Conditions in the Water Column of the Ferruginous Meromictic Lake La Cruz (Spain)

Distribution of sediment bacterial and archaeal communities in plateau freshwater lakes

The Distribution Pattern of Sediment Archaea Community of the Poyang Lake, the Largest Freshwater Lake in China

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