Aerobic bacteriological profile and antimicrobial susceptibility pattern of pus isolates from tertiary care hospital in India

Abstract

Introduction: Pyogenic infections are an important cause of sepsis. These infections are difficult to treat because of the pathogens with increasing antibiotic resistance. It is important to know the pathogens causing the infections and its antibiotic susceptibility for proper management of the patients.
 Methodology: A retrospective analysis of 1428 culture positive pus and tissue samples received in the department of microbiology from various departments in the hospital between January 2012 to 2017 was performed. Data regarding the pathogen isolated and its antimicrobial susceptibility were collected and analyzed. The specimens were primarily processed, as per standard methods. Identification and susceptibility testing was done using the Vitek-2C system.
 Results: Among the samples males outnumbered females (M: F-2.5:1) and the median age was 47 years. The total number of patients were 1428 with total number of isolates being 1525 as in our study monomicrobial infections were seen in 93.2% (1331/1428) patients whereas combined infections with growth of two pathogens in 6.8% (97/1428). Gram-negative bacilli were isolated in 68.3% (1042/1525). Among the Gram-negative bacilli Escherichia coli was the major pathogen isolated (38.6%, 403/1042). Gram positive organisms were isolated in 31.6% (483/1525) of cases and Staphylococcus aureus was the predominant organism isolated (91.7%, 443/483). Rare pathogens like Burkholderia pseudomallei in 3 patients and Nocardia in one patient were also isolated.
 Conclusion: This study emphasizes to understand the common organisms isolated from wound infections and it helps in empirical treatment of patients based on antibiotic susceptibility patterns.

Identification of Bacterial Isolates and Their Antimicrobial Susceptibility Pattern from Wound/Pus Sample in a Tertiary Care Hospital, Gwalior, India

“…In the present study, monomicrobial infections predominated (90.19%), while polymicrobial infections were observed (9.80%) ( Tables 3 and 4 ). The study by Sudhaharan et al 11 found that monomicrobial infection was 93.2% and polymicrobial infection was 6.8%; this result is consistent with our findings.…”

“…10 The loss of skin integrity due to a variety of reasons creates an environment conducive to the colonization and proliferation of microorganisms. 11 Humidity, heat, and nutrition in the wound attract pathogen from the cutaneous surface, environment, or the patient's own flora, which grow and release various virulence factors, resulting in wound infection. 12 Immune cells are recruited to the infection site by the body's defense mechanism to fight pathogens.…”

Identification and Characterization of Burkholderia pseudomallei from Localized Pyogenic Infections in Eastern India: A Clinico-Microbiological Study

“…23 A study in South India between January 2012 and 2017 shows B. pseudomallei as a rare pathogen in only three pus specimens. 24 Thus, the current study shows the emergence of B. pseudomallei as a significant pathogen in localized pyogenic infections in eastern India, distributed in various districts of Odisha and West Bengal. The apparent concentration of maximum number of cases in Khordha district may be because our hospital is situated in Khordha district of Odisha, and hence the likelihood of concentration of more cases in Khordha district.…”

Bacteriological profile and antibiotic susceptibility patterns of wound infections in a tertiary care hospital in South India

“…27 The antibiotic susceptibility patterns of all isolates were studied. The antibiotic panel for Gram positive cocci, Gram 28 However, the Gram positive cocci were resistant to commonly used antibiotics like amoxicillin with clavulanic acid, ciprofloxacin and pencillin. This difference could be due to better hospital infection control practices followed in our hospital.…”