Aerobic Batch Degradation of Cresol by  Newly Isolated Pseudomonas monteilii Cr13

Shainy, NK; Usha, R.

doi:10.22207/jpam.12.1.37

Cited by 4 publications

(2 citation statements)

References 28 publications

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“…Out of many bacteria isolated, only one species was able to degrade high concentration of o-cresol. This bacteria was identified based on the methods described in Bergey's manual of determinative bacteriology and diagnostic microbiology based on its morphological, cultural and biochemical characteristics and further confirmed based on the 16S rRNA sequence analysis and BLAST identification as Pseudomonas monteilii CR13 [11] in our laboratory. The culture was maintained by weekly subculturing with 500 mg/L of o-cresol and kept in 4 °C.…”

Section: Introductionmentioning

confidence: 82%

Optimization of o-cresol degrading microorganism and kinetics of degradation

Shainy¹,

Usha

2018

Indones. J. Chem.

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In the present study, Pseudomonas monteilii CR13 isolated from petroleum contaminated soil demonstrated the highest specific o-cresol degradation rate at all tested o-cresol concentrations and also was not disturbed by the starting substrate concentration used (o-cresol-500 mg/L). After a serial transfer of the isolate into a series of increasing o-cresol level, the organism demonstrated significant improvement on degradation ability up to 3000 mg/L. The optimum condition for the cell mass increase and biodegradation of o-cresol by Pseudomonas monteilii was in the minimal mineral medium of 3 at a pH of 6.5 and temperature 30 °C, stirring velocity of 160 rpm, and the substrate concentration of 500 mg/L. The biodegradation kinetic study was carried out by bacteria in different initial substrate concentrations (500–3000 mg/L). In the present test the μmax, Ks and the μ were found 0.332 h-1, 0.166 mg/L and 0.0282 mg/L for 500 mg/L of o-cresol, respectively. The organism is highly promising and could be used to remove high concentrations of o-cresol from highly polluted aquatic and soil regions. The cells could be immobilized on a suitable matrix and the efficiency of degradation could be effectively improved.

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Section: Introductionmentioning

confidence: 82%

Optimization of o-cresol degrading microorganism and kinetics of degradation

Shainy¹,

Usha

2018

Indones. J. Chem.

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“…The effective bacterial destructors of cresols described in the last ten years are, e.g., Serratia marcescens ABHI001 [34], Gliomastix indicus MTCC 3869 [35], Stenotrophomonas sp. [36], Pseudomonas monteilii CR13 [37], and Alcaligenes faecalis [38], which are able to utilize cresols from 10-1500 mg/L in different experimental conditions and at different times. In all of them, the importance of the initial concentration of cresol for its successful and complete degradation was noted.…”

Section: Discussionmentioning

confidence: 99%

Effects of Aromatic Compounds Degradation on Bacterial Cell Morphology

Gerginova,

Spankulova,

Paunova-Krasteva

et al. 2023

Fermentation

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The aim of the present study was to evaluate in parallel the capacity of three bacterial strains originating from oil-polluted soils to degrade monoaromatic compounds and the alterations in the bacterial cell morphology as a result of the biodegradation. The strain Gordonia sp. 12/5 can grow well in media containing catechol, o-, m-, and p-cresol without significant morphological changes in the cells, as shown by scanning electron microscopy. This implies good adaptation of the strain for growth in hydrocarbon-containing media and indicates it is a proper candidate strain for further development of purification methodologies applicable to ecosystems contaminated with such compounds. The growth of the two Rhodococcus strains in the presence of the above carbon sources is accompanied by changes in cell size characteristic of stress conditions. Nevertheless, their hydrocarbon-degrading capacity should not be neglected for future applications. In summary, the established ability to degrade monoaromatic compounds, in parallel with the morphological changes of the bacterial cells, can be used as a valuable indicator of the strain’s vitality in the presence of tested aromatic compounds and, accordingly, of its applicability for bioremediation purposes.

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