Aerobic biodegradation kinetics of four anionic and nonionic surfactants at sub- and supra-critical micelle concentrations (CMCs)

Zhang, Chunlong; Valsaraj, Kalliat T.; Constant, W. David; Roy, Dipak

doi:10.1016/s0043-1354(98)00170-5

Cited by 107 publications

(59 citation statements)

References 23 publications

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“…One commonly cited reason for inhibition is surfactant toxicity (3,25,26,33). Witconol SN70, a nonionic, alcohol ethoxylate, was chosen both for its prior success in enhancing pyrene mineralization (24) and its biodegradability (12,34). Macur and Inskeep (15) previously suggested that total CO 2 evolution data did not indicate gross toxicity of Witconol SN70 to all organisms in this soil.…”

Section: Fig 3 Denaturing Gradient Gel (40 To 60% Uf) Of Pcr-amplifmentioning

confidence: 99%

Molecular Analysis of Surfactant-Driven Microbial Population Shifts in Hydrocarbon-Contaminated Soil

Colores

Macur

Ward

et al. 2000

Appl Environ Microbiol

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We analyzed the impact of surfactant addition on hydrocarbon mineralization kinetics and the associated population shifts of hydrocarbon-degrading microorganisms in soil. A mixture of radiolabeled hexadecane and phenanthrene was added to batch soil vessels. Witconol SN70 (a nonionic, alcohol ethoxylate) was added in concentrations that bracketed the critical micelle concentration (CMC) in soil (CMC) (determined to be 13 mg g ؊1 ). Addition of the surfactant at a concentration below the CMC (2 mg g ؊1 ) did not affect the mineralization rates of either hydrocarbon. However, when surfactant was added at a concentration approaching the CMC (10 mg g ؊1 ), hexadecane mineralization was delayed and phenanthrene mineralization was completely inhibited. Addition of surfactant at concentrations above the CMC (40 mg g ؊1 ) completely inhibited mineralization of both phenanthrene and hexadecane. Denaturing gradient gel electrophoresis of 16S rRNA gene segments showed that hydrocarbon amendment stimulated Rhodococcus and Nocardia populations that were displaced by Pseudomonas and Alcaligenes populations at elevated surfactant levels. Parallel cultivation studies revealed that the Rhodococcus population can utilize hexadecane and that the Pseudomonas and Alcaligenes populations can utilize both Witconol SN70 and hexadecane for growth. The results suggest that surfactant applications necessary to achieve the CMC alter the microbial populations responsible for hydrocarbon mineralization.Surfactants have been successfully used to enhance the apparent solubility of nonpolar organic contaminants (NOC) as well as their subsequent removal from soil. There is, however, a great deal of conflicting information regarding the ability of surfactants to enhance the bioavailability and biodegradation of NOC (10, 21, 28). For most surfactants, an increase in NOC solubility is achieved only at surfactant concentrations greater than the critical micelle concentration (CMC), where the majority of surfactant molecules are aggregated into micelles. Surfactant micelles contain a hydrophobic core with a high affinity for NOC, resulting in increases in the apparent solubility of NOC. However, surfactant application at concentrations approaching and exceeding the CMC often result in significant decreases in rates of microbial NOC degradation. Potential mechanisms of inhibition include reduction in NOC bioavailability when bound in surfactant micelles (9, 13, 27), inhibition of microbial attachment at mineral and organic surfaces (4, 7, 23), and surfactant toxicity (3,25,26,33). With a few exceptions, the majority of studies investigating the influence of surfactants on NOC biodegradation involve pure cultures. Furthermore, those using mixed cultures primarily address the impact of surfactant dose on the kinetics of NOC degradation. To our knowledge, there has been no attempt to evaluate changes in microbial community structure across a range of surfactant applications. Consequently, the goal of this study was to examine shifts of NOC-degrading popul...

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Section: Fig 3 Denaturing Gradient Gel (40 To 60% Uf) Of Pcr-amplifmentioning

confidence: 99%

Molecular Analysis of Surfactant-Driven Microbial Population Shifts in Hydrocarbon-Contaminated Soil

Colores

Macur

Ward

et al. 2000

Appl Environ Microbiol

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“…Zhang et al (1999) observed that primary biodegradation of a nonionic surfactant above the CMC was first order, while at sub-CMC concentrations was described by the Monod equation. Swisher (1987) summarizes similar findings for anionic surfactant biodegradation, which followed Michaelis-Menten-type relationships below the CMC and then decreasing degradation rates at higher concentrations.…”

Section: Model Development and Testingmentioning

confidence: 99%

Dual substrate biodegradation of a nonionic surfactant and pentachlorophenol by Sphingomonas chlorophenolica RA2

Bielefeldt

Cort

2005

Biotech & Bioengineering

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The simultaneous biodegradation of the nonionic surfactant Tween 20 (Tw20) and pentachlorophenol (PCP) by Sphingomonas chlorophenolica sp. Strain RA2 (RA2) was measured. As a sole substrate, Tw20 biodegradation was best described by the Contois kinetic model. During concurrent biodegradation of Tw20 and PCP, the biodegradation rates of Tw20 were not significantly affected by 50 or 100 mg/L PCP, but were significantly inhibited by 500 mg/L PCP. Decreases in cell yield in the presence of PCP suggest that PCP was acting as an uncoupler. Cultures were pre-grown on PCP or Tw20 before degradation of PCP to evaluate enzyme induction effects, and long lags before PCP biodegradation after growth on Tw20 occurred. Although biokinetic models could accurately describe some of the data sets of RA2 growth and Tw20 and PCP degradation, finding a single set of kinetic parameters that predicted all dual substrate tests was not achieved. The complicating factors to modeling PCP and Tw20 interactions are described and may be more widely applicable to the biodegradation of toxic organic compounds in the presence of a biodegradable surfactant.

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“…It may also lead to health hazards in the form of air borne pathogens carried on windblown foams. Presence of LAS at a concentration 500 mg/L is reported to inhibit microbial growth and resist biodegradation [3]. Most of the LAS biodegradation studies were conducted under aerobic condition or in anaerobic condition preceded by a period of aerobic exposure.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Linear Alkylbenzene Sulfonate (LAS) by Immobilized <i>Pseudomonas</i> sp.

Asok¹,

Fathima²,

Jisha³

2015

ACES

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Anionic surfactants are the most widely exploited chemical surfactants, which are being incorporated into majority of detergents and cleaning products used for household and industrial applications. Linear alkylbenzene sulfonates (LAS) is one of the major xenobiotic anionic surfactants. Biodegradation is an effective process to reduce the amount of surfactants released in the environment. In this study soil samples were collected from detergent contaminated sites to isolate linear alkylbenzene sulphonate degrading bacteria using mineral salt media supplemented with LAS as sole source of carbon. From the twenty isolates selected for the study, two of them (L9 and L12) have exhibited excellent ability to degrade LAS. The LAS degradation ability was determined by using MBAS assay and HPLC. The selected isolates were immobilized in alginate and polyvinyl alcohol to check the suitability of the isolates in onsite LAS removal. The percentage of degradation of LAS by alginate entrapped L9 was 85.155 ± 1.2 and that of PVA immobilized cells was 58.535 ± 2.9. Whereas L12 PVA entrapped were good compared to alginate. L12 exhibited 62.977 ± 1.3 percentage of degradation of LAS when immobilized in PVA and 61.07 ± 0.6 percentage in alginate entrapped condition. In comparison between the organisms alginate entrapped Pseudomonas nitroreducens (L9) was found to be superior to Pseudomonas aeruginosa (L12) in immobilised condition. It was found that immobilized cells performed superiorly than free cells. In particular, calcium alginate immobilised cells were more efficient in LAS removal than polyvinyl alcohol immobilised cells.

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Aerobic biodegradation kinetics of four anionic and nonionic surfactants at sub- and supra-critical micelle concentrations (CMCs)

Cited by 107 publications

References 23 publications

Molecular Analysis of Surfactant-Driven Microbial Population Shifts in Hydrocarbon-Contaminated Soil

Molecular Analysis of Surfactant-Driven Microbial Population Shifts in Hydrocarbon-Contaminated Soil

Dual substrate biodegradation of a nonionic surfactant and pentachlorophenol by Sphingomonas chlorophenolica RA2

Biodegradation of Linear Alkylbenzene Sulfonate (LAS) by Immobilized <i>Pseudomonas</i> sp.

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Aerobic biodegradation kinetics of four anionic and nonionic surfactants at sub- and supra-critical micelle concentrations (CMCs)

Cited by 107 publications

References 23 publications

Molecular Analysis of Surfactant-Driven Microbial Population Shifts in Hydrocarbon-Contaminated Soil

Molecular Analysis of Surfactant-Driven Microbial Population Shifts in Hydrocarbon-Contaminated Soil

Dual substrate biodegradation of a nonionic surfactant and pentachlorophenol by Sphingomonas chlorophenolica RA2

Biodegradation of Linear Alkylbenzene Sulfonate (LAS) by Immobilized &lt;i&gt;Pseudomonas&lt;/i&gt; sp.

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Biodegradation of Linear Alkylbenzene Sulfonate (LAS) by Immobilized <i>Pseudomonas</i> sp.