Aerobic biodegradation of dichloroethylenes in surface and subsurface soils

Klier, N.J.; West, Robert J.; Donberg, P.A.

doi:10.1016/s0045-6535(98)00485-8

Cited by 37 publications

(32 citation statements)

References 25 publications

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“…Thermodynamic calculations suggest that cDCE contains sufficient energy to support aerobic growth (4), and enzymes active on cDCE are known in hydrocarbonoxidizing bacteria (5,6,13,20,24,25). In addition, aerobic oxidation of cDCE to CO 2 has been observed in microcosm and enrichment culture studies (2,12). Encouraged by the facts described above, we hypothesized that aerobic growth on cDCE was possible and searched at a variety of contaminated sites for microorganisms able to use this compound as a sole source of carbon and energy.…”

mentioning

confidence: 92%

Biodegradation of cis -Dichloroethene as the Sole Carbon Source by a β-Proteobacterium

Coleman

Mattes

Gossett

et al. 2002

Appl Environ Microbiol

199

152

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An aerobic bacterium capable of growth on cis-dichloroethene (cDCE) as a sole carbon and energy source was isolated by enrichment culture. The 16S ribosomal DNA sequence of the isolate (strain JS666) had 97.9% identity to the sequence from Polaromonas vacuolata, indicating that the isolate was a ␤-proteobacterium. At 20°C, strain JS666 grew on cDCE with a minimum doubling time of 73 ؎ 7 h and a growth yield of 6.1 g of protein/mol of cDCE. Chloride analysis indicated that complete dechlorination of cDCE occurred during growth. The half-velocity constant for cDCE transformation was 1.6 ؎ 0.2 M, and the maximum specific substrate utilization rate ranged from 12.6 to 16.8 nmol/min/mg of protein. Resting cells grown on cDCE could transform cDCE, ethene, vinyl chloride, trans-dichloroethene, trichloroethene, and 1,2-dichloroethane. Epoxyethane was produced from ethene by cDCE-grown cells, suggesting that an epoxidation reaction is the first step in cDCE degradation.The extensive use of chloroethenes as solvents and synthetic feedstocks has resulted in widespread environmental contamination (22), which is of concern due to the toxicity and carcinogenicity of such compounds. Microbial metabolism is an important factor in determining the fate of chloroethenes in the biosphere (14). Several anaerobic bacteria use tetrachloroethene (perchloroethene) and trichloroethene (TCE) as electron acceptors, producing cis-1,2-dichloroethene (cDCE), vinyl chloride (VC), and ethene as end products (10,15,16,17). Under aerobic conditions, ethene and VC can serve as carbon and energy sources for bacterial growth (3, 8), but thus far, no conclusive evidence exists for aerobic growth on any of the dichloroethenes (cis-dichloroethene, trans-dichloroetheneBecause cDCE accumulation is often a limiting factor in the biodegradation of chloroethenes in subsurface ecosystems, aerobic bacteria capable of growth on cDCE would provide a crucial missing link in the chain of microbial metabolism for this class of compounds. Thermodynamic calculations suggest that cDCE contains sufficient energy to support aerobic growth (4), and enzymes active on cDCE are known in hydrocarbonoxidizing bacteria (5,6,13,20,24,25). In addition, aerobic oxidation of cDCE to CO 2 has been observed in microcosm and enrichment culture studies (2, 12). Encouraged by the facts described above, we hypothesized that aerobic growth on cDCE was possible and searched at a variety of contaminated sites for microorganisms able to use this compound as a sole source of carbon and energy. MATERIALS AND METHODSChemicals and media. cis-Dichloroethene (97%), tDCE (98%), TCE (99.5%), and 1,2-dichloroethane (1,2-DCA) (99.8%) were obtained from Sigma-Aldrich. VC (99.5%) was obtained from Fluka, and ethene (99.5%) was obtained from Scott. All other chemicals were reagent grade. A minimal salts medium (MSM) based on that of Hartmans et al. (9) was used for enrichment cultures, with the following modifications: the phosphate concentration was reduced to 20 mM, the ammonium concentration was red...

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mentioning

confidence: 92%

Biodegradation of cis -Dichloroethene as the Sole Carbon Source by a β-Proteobacterium

Coleman

Mattes

Gossett

et al. 2002

Appl Environ Microbiol

199

152

View full text Add to dashboard Cite

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“…En raison de leur potentiel redox, ces deux molécules peuvent aussi être dégradées par oxydation en C0 2 en milieu aéro-bie. La dégradation de DCE n'a été constatée qu'en milieu aérobie (KLIER et al, 1999) alors que le VC peut se dégrader en conditions aérobies et Fe-réduc-trices (BRADLEY ef al., 1998 ;, la réaction n'a pas été montrée en conditions nitratoréductrices.…”

Section: Réactionsunclassified

Conditions chimiques contrôlant l'atténuation naturelle des BTEX et solvants chlorés : un état des connaissances

Atteia¹,

Franceschi²

2005

rseau

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L'atténuation naturelle des BTEX (Benzène, Toluène, Ethyl-benzène, Xylène) et des solvants chlorés est de plus en plus étudiée en raison des potentialités offertes par cette technique de gestion. Cet article, après avoir présenté les aspects abiotiques de l'atténuation détaille les conditions chimiques nécessaires à la réalisation des réactions de biodégradation des polluants organiques. Les aspects thermodynamiques sont abordés afin de décliner les réactions possibles et celles qui ne le sont pas selon les environnements chimiques. La dégradation des BTEX est focalisée sur le benzène, produit le plus toxique et le moins dégradable sur la plupart des sites. Les détails de la dégradation du benzène sur le terrain sont analysés dans la littérature et leur comparaison permet de décrire les mécanismes responsables de celle-ci. Dans le cas des solvants chlorés, l'attention est portée sur le TCE (Trichloréthylène), produit le plus couramment rencontré sur les sites pollués. Une mise en parallèle des évolutions de teneurs observées et des conditions chimiques locales permet de mettre en évidence les conditions nécessaires à la dégradation du TCE, et de ses congénères, ainsi que les cinétiques de dégradation dans différentes conditions. La mise en évidence du rôle prépondérant des conditions chimiques conduit à remettre en cause l'utilisation répandue des constantes de dégradation du premier ordre et donne des pistes pour les modèles nécessaires à une prédiction plus fine de l'atténuation naturelle.The increasing reliance on natural attenuation in dealing with contaminated sites in North America is the consequence of:1. the extremely long duration and high cost of aquifer rehabilitation by classical methods, and 2. the discovery of natural biodegradation in many different situations. However, the use of this management technique is questionable, as intrinsic biodegradation is highly dependent on chemical conditions and particularly on redox equilibria. This paper describes the role of these chemical conditions on BTEX and chlorinated solvent attenuation and, by analyzing the current research, we try to define current limits of the predictability of natural attenuation in field conditions.Natural attenuation is defined as the sum of processes able to decrease the pollutant concentration at a sampling point in an aquifer. Several physical processes such as dispersion, retardation and solubility play a role in natural attenuation. However, only biodegradation can significantly reduce the overall amount of pollutants in an aquifer, thereby allowing the pollutant concentration to reach the low levels that are required by regulations. The physical processes cited above can be modelled at a site to account for their effect, but the main focus is on biodegradation.A detailed analysis of the basic thermodynamics of redox reactions involved in biodegradation is necessary to describe the reactions that can potentially occur. A rough analysis shows that BTEX is mainly degraded by oxidation and therefore is degraded more efficiently ...

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“…In addition, Bradley and Chapelle (1996) show evidence of oxidation of VC under iron-reducing conditions so long as there is sufficient bioavailable iron (III). Klier et al (1996) write that naturally occurring microorganisms in soil and groundwater are capable of biodegrading DCE by using this compound as a primary substrate (i.e. an electron donor).…”

Section: Electron Donor Reactionsmentioning

confidence: 99%

Treatability Study in Support of Intrinsic Remediation for the Fire Training Area (Site 3). Michigan Air National Guard at W. K. Kellogg Memorial Airport, Battle Creek Michigan

CO¹

1999

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Aerobic biodegradation of dichloroethylenes in surface and subsurface soils

Cited by 37 publications

References 25 publications

Biodegradation of cis -Dichloroethene as the Sole Carbon Source by a β-Proteobacterium

Biodegradation of cis -Dichloroethene as the Sole Carbon Source by a β-Proteobacterium

Conditions chimiques contrôlant l'atténuation naturelle des BTEX et solvants chlorés : un état des connaissances

Treatability Study in Support of Intrinsic Remediation for the Fire Training Area (Site 3). Michigan Air National Guard at W. K. Kellogg Memorial Airport, Battle Creek Michigan

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