OBJECTIVE: To examine whether body fat content in pre-pubertal children is in¯uenced by physical activity related energy expenditure (AEE) and/or more qualitative aspects of physical activity. DESIGN: Cross-sectional study. SUBJECT: 101 pre-pubertal children were examined in Study 1: (age: 5.3 AE 0.9 y; weight: 20.2 AE 3.6 kg). In Study 2: 68 of the original children were re-examined (age: 6.3 AE 0.9 y; weight: 23.6 AE 5.0 y). MEASUREMENT: Fat mass (FM) and fat free mass (FFM) were determined by bioelectrical resistance and skinfolds; AEE was estimated from the difference between total energy expenditure (TEE) by doubly labeled water and postprandial resting energy expenditure (REE) by indirect calorimetry; qualitative information on activity was derived by questionnaire. RESULTS: AEE was signi®cantly correlated with FFM (r 0.32 in both Studies) and body weight (r 0.28 in Study 1; r 0.29 in Study 2), but not FM. There were no signi®cant relationships between AEE and any of the variables from the activity questionnaire in children (including TV time, playing time, and an accumulated activity index in h/ week). After adjusting for FFM, age, and gender, FM was inversely related to activity time in h/week (partial r 70.24 in Study 1; partial r 70.32 in Study 2) but not AEE (P b 0.5). CONCLUSION: After adjusting for FFM, age, and gender, a small portion of the variance in body fat mass in children ( $ 10%) is explained by time devoted to recreational activity, whereas none of the variance is explained by the combined daily energy expenditure related to physical activity.