Aerobic degradation of methyl tert-butyl ether by a Proteobacteria strain in a closed culture system

Zhong, Weihong; Chen, Jianmeng; Zheng, Lu; Chen, Dong-zhi; Xiao, Chen

doi:10.1016/s1001-0742(07)60003-5

Cited by 12 publications

(12 citation statements)

References 24 publications

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“…As a result of the rapidly increasing production and consumption of unleaded gasoline in China [3], many groundwater sources for major Chinese cities will eventually be contaminated by MTBE because of its high water solubility, relatively low volatility, and low biodegradability. Although MTBE has been biodegraded by acclimated bacteria [8][9][10][11], it is unlikely that any form of biotreatment by itself can produce a treated effluent meeting the discharge limit of 20 lg/L or lower [12][13][14]. Although MTBE has been biodegraded by acclimated bacteria [8][9][10][11], it is unlikely that any form of biotreatment by itself can produce a treated effluent meeting the discharge limit of 20 lg/L or lower [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Removal of MTBE in biological activated carbon adsorbers

Huang

et al. 2012

Env Prog and Sustain Energy

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Methyl tert‐butyl ether (MTBE) is a common gasoline additive; it has become a groundwater pollutant in many countries. Granular activated carbon (GAC) adsorption treatment is not cost‐effective for removing MTBE because it is not well adsorbed. Acclimated MTBE degraders actively growing in the GAC adsorber will make it a biological activated carbon (BAC) system capable of long‐term MTBE removal without periodic GAC replacement. The conventional inoculation method of circulating a seeding solution of the MTBE degraders present in the spent GAC sample from a BAC system of a southern California MTBE remediation site failed to initiate the BAC function in treating a high MTBE influent (30 mg/L, simulating a newly contaminated groundwater); the effluent samples of inoculated coal and coconut GAC columns were nearly the same as those of the non‐inoculated columns. After a start‐up period of less than two months, the small GAC columns filled with new coconut GAC on top of the MTBE degrader containing spent GAC became effective BAC systems accomplishing more than 40% of MTBE removal from the high concentration (30 mg/L) influent and more than 80% of removal from the low concentration (1 mg/L) influent. When MTBE concentration of the influent declined steadily, the BAC capability prevented the sudden rise of MTBE in the effluent. A small dose of hydrogen peroxide provided the essential dissolved oxygen to sustain aerobic degradation of MTBE in the BAC; adding peroxide to the influents of two serial adsorbers is more efficient in treating newly contaminated groundwater. © 2012 American Institute of Chemical Engineers Environ Prog, 32: 239‐248, 2013

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Section: Introductionmentioning

confidence: 99%

Removal of MTBE in biological activated carbon adsorbers

Huang

et al. 2012

Env Prog and Sustain Energy

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“…When the DO level was kept at 4 mg/L, the removal efficiency was >98%. Zhong et al (2007) reported that PM1 biodegraded MTBE quickly under the aerobic condition and that sufficient oxygen improved MTBE degradation activity. The first step in MTBE biodegradation is catalyzation by monooxygenase, which is one of the key enzymes in this pathway (Johnson et al 2004).…”

Section: Effect Of Domentioning

confidence: 99%

“…The prevalent use of methyl tert-butyl ether (MTBE) in reformulated fuel to further reduce emissions of carbon monoxide and other smog-related air pollutants has led to its introduction into groundwater from spills and leaky underground storage tanks (Baehr et al 1999;Klinger et al 2002;Chiu et al 2006;Dong et al 2007;Zhong et al 2007 ). In the USA, Landmeyer et al (2001) tested all of the wells adjacent to an MTBE storage tank and found MTBE to be present at the milligram per liter level, with even concentrations >10 mg/L MTBE being found.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Methyl Tert-butyl Ether in a Bioreactor using Immobilized Methylibium petroleiphilum PM1 Cells

Cheng

Chen

et al. 2010

Water Air Soil Pollut

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Methylibium petroleiphilum PM1, which is capable of degrading of methyl tert-butyl ether (MTBE), was immobilized in calcium alginate gel beads. Various applications were explored to increase the mechanical strength of these gel beads. The introduction of 0.3 mol/L calcium chloride into the crosslinking solution, 0.002 mol/L calcium chloride into the growth medium, and 0.2% polyethyleneimine (PEI) as chemical crosslinking agent increased the stability of the Ca-alginate gel beads under the operation conditions of the bioreactor. The degradation rates of MTBE by the immobilized cells in the bioreactor system operated in batch and continuous mode , respectively, were compared. A MTBE biodegradation rate of 5.79 mg/L·h was reached for over 400 h (50 batches), and the immobilized cells in the bioreactor removed >96% MTBE during 50 days of operation. Molecular analysis of the PM1 cells revealed that microbial growth occurred predominantly as microcolonies in the outer area of the beads during the first 20 days of operation. The results of this study show that a continuous-mode, fixed-bed bioreactor reactor coupled with PM1-immobilized cells is a promising technology for remediating MTBE-contaminated groundwater.

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“…In our previous work, a "cannula" was introduced to study MTBE biodegradation in a closed system [19]. A high level of DO in the broth within the inner tube was achieved by the addition of H 2 O 2 in the outer tube of the cannula.…”

Section: Introductionmentioning

confidence: 99%