Aerobic denitrification and heterotrophic nitrification byThiosphaera pantotropha

Robertson, L.A.; Kuenen, J.G.; Kleijntjens, R. H.

doi:10.1007/bf02275068

Cited by 30 publications

(15 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results were obtained with Thiosphaera pantotropha [19], Bacillus sp. [6], and Pseudomonas sp.…”

Section: Discussionsupporting

confidence: 78%

“…These microorganisms, due to their high growth rate and ability to convert ammonium (NH 4 ? ) to N 2 aerobically, have many advantages as applied for the removal of nitrogen: (1) procedural simplicity, where nitrification and denitrification can take place simultaneously; (2) less acclimation problems; (3) lesser buffer quantity needed because alkalinity generated during denitrification can partly compensate for the alkalinity consumption in nitrification [3]. To date, certain groups of heterotrophic nitrification-aerobic denitrification bacteria, such as Thiosphaera pantotropha, Alcaligenes faecalis, Bacillus sp., and Providencia rettgeri have been isolated from soils and wastewater treatment systems [1,11,19,29].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Heterotrophic nitrification–aerobic denitrification by novel isolated bacteria

Chen

2010

J Ind Microbiol Biotechnol

173

View full text Add to dashboard Cite

Three novel strains capable of heterotrophic nitrification-aerobic denitrification were isolated from the landfill leachate treatment system. Based on their phenotypic and phylogenetic characteristics, the isolates were identified as Agrobacterium sp. LAD9, Achromobacter sp. GAD3 and Comamonas sp. GAD4, respectively. Batch tests were carried out to evaluate the growth and the ammonia removal patterns. The maximum growth rates as determined from the growth curve were 0.286, 0.228, and 0.433 h(-1) for LAD9, GAD3 and GAD4, respectively. The maximum aerobic nitrification-denitrification rate was achieved by the strain GAD4 of 0.381 mmol/l h, followed by LAD9 of 0.374 mmol/l h and GAD3 of 0.346 mmol/l h. Moreover, hydroxylamine oxidase and periplasmic nitrate reductase were successfully expressed in all the isolates. The relationship between the enzyme activities and the aerobic nitrification-denitrification rates revealed that hydroxylamine oxidation may be the rate-limiting step in the heterotrophic nitrification-aerobic denitrification process. The study results are of great significance to the wastewater treatment systems where simultaneous removal of carbon and nitrogen is desired.

show abstract

“…Similar results were obtained with Thiosphaera pantotropha [19], Bacillus sp. [6], and Pseudomonas sp.…”

Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Heterotrophic nitrification–aerobic denitrification by novel isolated bacteria

Chen

2010

J Ind Microbiol Biotechnol

173

View full text Add to dashboard Cite

show abstract

“…Earlier it was believed by most researchers that heterotrophs could carry out denitrification only under anoxic conditions (Guo et al ). It was in 1985, when Robertson for the first time reported aerobic denitrification by the bacterium Thiosphaera pantotropha (Robertson et al ). Conventional biological methods for nitrogen removal from wastewaters were a stepwise procedure involving nitrifying bacteria which carried out nitrification under oxic conditions, followed by heterotrophic denitrifiers which performed denitrification under anoxic conditions (Joo et al ).…”

Section: Biological Routes Of Nitrate Removalmentioning

confidence: 99%

Role of heterotrophic aerobic denitrifying bacteria in nitrate removal from wastewater

et al. 2020

View full text Add to dashboard Cite

With the increase in industrial and agricultural activities, a large amount of nitrogenous compounds are released into the environment, leading to nitrate pollution. The perilous effects of nitrate present in the environment pose a major threat to human and animal health. Bioremediation provides a costeffective and environmental friendly method to deal with this problem. The process of aerobic denitrification can reduce nitrate compounds to harmless dinitrogen gas. This review provides a brief view of the exhaustive role played by aerobic denitrifiers for tackling nitrate pollution under different ecological niches and their dependency on various environmental parameters. It also provides an understanding of the enzymes involved in aerobic denitrification. The role of aerobic denitrification to solve the issues faced by the conventional method (aerobic nitrification-anaerobic denitrification) in treating nitrogenpolluted wastewaters is elaborated. verts the iron centre of haemoglobin from Fe 2+ to Fe 3+ Role of heterotrophic aerobic denitrifying bacteriaA. Rajta et al.

show abstract

“…Robertson and Kuenen’s discovery of aerobic denitrification bacteria Thiosphaera pantotropha [ 14 ] at a denitrifying, sulfide-oxidizing wastewater treatment plant, demonstrated a novel method of nitrogen removal, which is not limited to oxygen [ 14 , 15 , 16 ]. Microbiologists have defined aerobic denitrification as the co-respiration or co-metabolism of oxygen and nitrate [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Nitrogen Removal Characteristics of a Newly Isolated Indigenous Aerobic Denitrifier from Oligotrophic Drinking Water Reservoir, Zoogloea sp. N299

Huang

Zhou

Zhang

et al. 2015

IJMS

View full text Add to dashboard Cite

Nitrogen is considered to be one of the most widespread pollutants leading to eutrophication of freshwater ecosystems, especially in drinking water reservoirs. In this study, an oligotrophic aerobic denitrifier was isolated from drinking water reservoir sediment. Nitrogen removal performance was explored. The strain was identified by 16S rRNA gene sequence analysis as Zoogloea sp. N299. This species exhibits a periplasmic nitrate reductase gene (napA). Its specific growth rate was 0.22 h−1. Obvious denitrification and perfect nitrogen removal performances occurred when cultured in nitrate and nitrite mediums, at rates of 75.53% ± 1.69% and 58.65% ± 0.61%, respectively. The ammonia removal rate reached 44.12% ± 1.61% in ammonia medium. Zoogloea sp. N299 was inoculated into sterilized and unsterilized reservoir source waters with a dissolved oxygen level of 5–9 mg/L, pH 8–9, and C/N 1.14:1. The total nitrogen removal rate reached 46.41% ± 3.17% (sterilized) and 44.88% ± 4.31% (unsterilized). The cell optical density suggested the strain could survive in oligotrophic drinking water reservoir water conditions and perform nitrogen removal. Sodium acetate was the most favorable carbon source for nitrogen removal by strain N299 (p < 0.05). High C/N was beneficial for nitrate reduction (p < 0.05). The nitrate removal efficiencies showed no significant differences among the tested inoculums dosage (p > 0.05). Furthermore, strain N299 could efficiently remove nitrate at neutral and slightly alkaline and low temperature conditions. These results, therefore, demonstrate that Zoogloea sp. N299 has high removal characteristics, and can be used as a nitrogen removal microbial inoculum with simultaneous aerobic nitrification and denitrification in a micro-polluted reservoir water ecosystem.

show abstract

Aerobic denitrification and heterotrophic nitrification byThiosphaera pantotropha

Cited by 30 publications

References 1 publication

Heterotrophic nitrification–aerobic denitrification by novel isolated bacteria

Heterotrophic nitrification–aerobic denitrification by novel isolated bacteria

Role of heterotrophic aerobic denitrifying bacteria in nitrate removal from wastewater

Nitrogen Removal Characteristics of a Newly Isolated Indigenous Aerobic Denitrifier from Oligotrophic Drinking Water Reservoir, Zoogloea sp. N299

Contact Info

Product

Resources

About