AEROBIC RESPIRATORY METABOLISM OF
            <i>STAPHYLOCOCCUS AUREUS</i>
            FROM AN INFECTED ANIMAL

Gellenbeck, S. Mercy

doi:10.1128/jb.83.3.450-455.1962

Cited by 11 publications

(5 citation statements)

References 1 publication

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“…This suspension represented approximately 6 X 109 viable cells per ml. A male guinea pig was injected, and the organisms recovered and purified as reported previously (Gellenbeck, 1962).…”

Section: Materials and Imethodsmentioning

confidence: 99%

“…Biochemical and metabolic studies. Aerobic respiratory rates of VSB and GSB cells in 0.0125 M glucose and in buffer (endogenous) were determined by the procedure of Gellenbeck (1962).…”

Section: Materials and Imethodsmentioning

confidence: 99%

“…Smith, Keppie, and Stanley (1953) established a workable method for collecting and separating large amounts of bacteria from the tissue fluid of infected guinea pigs. WVith this procedure, Gellenbeck (1962) showed that her in vitro strain of S. aureus had a significantly lower exogenous respiratory rate than did the same strain after it had passed through a guinea pig.…”

mentioning

confidence: 99%

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CHARACTERISTICS OF A STRAIN OFSTAPHYLOCOCCUS AUREUSGROWN IN VIVO AND IN VITRO

Beining

Kennedy

1963

J Bacteriol

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Characteristics of a strain of Staphylococcus aureus grown in vivo and in vitro. J. Bacteriol. 85:732-741. 1963.-A comparative survey was conducted on the characteristics of a strain of Staphylococcus aureus after it had been grown in vitro (VSB) and after it had been collected from the peritoneal exudate of an infected guinea pig (GSB). Both VSB and GSB strains gave the same results when studied in an extensive series of tests, including bound and soluble coagulases, bacteriophage type, antibioticsensitivity pattern, the usual fermentation reactions, deoxyribonucleic acid base composition, and qualitative tests for hemolysins, deoxyribonuclease, ribonuclease, staphylokinase, staphyloprotease, lipase, and phosphatase. The in vivo strain differed significantly from the in vitro strain in respiratory rate, agar gel diffusion studies, agglutinability in tube tests, virulence tests in rabbits and mice, growth on telluriteglycine agar, susceptibility to human-y-globulin in agar, and in the quantitative production of deoxyribonuclease, a-hemolysin, leucocidin, and hyaluronidase.

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“…This suspension represented approximately 6 X 109 viable cells per ml. A male guinea pig was injected, and the organisms recovered and purified as reported previously (Gellenbeck, 1962).…”

Section: Materials and Imethodsmentioning

confidence: 99%

“…Biochemical and metabolic studies. Aerobic respiratory rates of VSB and GSB cells in 0.0125 M glucose and in buffer (endogenous) were determined by the procedure of Gellenbeck (1962).…”

Section: Materials and Imethodsmentioning

confidence: 99%

mentioning

confidence: 99%

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CHARACTERISTICS OF A STRAIN OFSTAPHYLOCOCCUS AUREUSGROWN IN VIVO AND IN VITRO

Beining

Kennedy

1963

J Bacteriol

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“…Mycobacterium isolated from infected mouse tissues exhibit reduced oxidation capacity because of deficiency of cytochromes (6,13). Staphylococcus aureus grown in vivo, on the contrary, shows a higher state of oxidation activity and produces far more hemolysin, leucocidin, and tissue-attacking enzymes (1,4). Most pathogenic fungi show dual aspects of the morphology of fungi in their saprophytic and parasitic states (8).…”

mentioning

confidence: 99%

Fine Structure of Cryptococcus neoformans Grown In Vivo as Observed by Freeze-Etching

et al. 1973

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Cryptococcus neoformans grown in the parasitic state was observed by the freeze-etching technique and was compared with that grown on culture media. Unlike other yeasts, this organism grown in vivo is very often devoid of the "ordinary" invaginations. The membrane of the cell grown in vivo was almost free from concavity and convexity except for many round depressions which represent the surface view of paramural bodies. Some of the paramural bodies were found to be multivesicular systems. Most were spherical invaginations containing a single vesicle or its ghost remaining after secretion of the vesicles. In clear contrast to the cell grown in vitro, the in vivo cell contained a great number of vesicles in the cytoplasm. These seemed to show high-secretion activity in C. neoformans grown in the parasitic state. On transfer from in vitro to in vivo, this organism enlarged the cell wall, capsule, and cell body. The appearance of a large vacuole, accumulation of storage organelles, and the existence of rodlike structures, seemingly lipid deposits, were also noted in the cytoplasm of the cell grown in vivo. The meaning of these results as well as the mode of capsular production are discussed.

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“…Our findings confirm the dependence of type 5 CP production by S. aureus on the bacterial cell's respiratory activity. Oxygen is subject to concentration fluctuations in the human host (36), and the aerobic respiration of S. aureus from tissue fluid is higher than that of bacteria grown in vitro (19). The influence of bacterial respiration on CP production might thus be of some importance in the process of infection.…”

Section: Discussionmentioning

confidence: 99%

Respiratory activity is essential for post-exponential-phase production of type 5 capsular polysaccharide by Staphylococcus aureus

Dassy

Fournier

1996

Infect Immun

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Capsule formation is believed to have a significant role in bacterial virulence. To examine the possible involvement of capsular polysaccharide (CP) from Staphylococcus aureus in the pathological mechanisms associated with staphylococcal infections, we investigated the influence of respiratory activity on type 5 CP production by S. aureus grown in the presence of various concentrations of dissolved oxygen or nitrate. The effects of several metabolic inhibitors (arsenite, cyanide, azide, trimethylamine N-oxide, 2-heptyl-4-hydroxyquinoline N-oxide, and 2,4-dinitrophenol) were also tested. The metabolism of the bacteria was estimated by measuring their reductive capacity and by monitoring the pH and concentrations of fermentation products. Type 5 CP was always produced by S. aureus during the exponential phase of growth under all culture conditions tested. In contrast, post-exponential-phase CP production appeared to be strictly dependent on the respiratory activity. Since post-exponential-phase CP production contributes at least two-thirds of the total CP obtained, the influence of S. aureus respiration on CP production might be of some importance in the process of infection.

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AEROBIC RESPIRATORY METABOLISM OF STAPHYLOCOCCUS AUREUS FROM AN INFECTED ANIMAL

Cited by 11 publications

References 1 publication

CHARACTERISTICS OF A STRAIN OFSTAPHYLOCOCCUS AUREUSGROWN IN VIVO AND IN VITRO

CHARACTERISTICS OF A STRAIN OFSTAPHYLOCOCCUS AUREUSGROWN IN VIVO AND IN VITRO

Fine Structure of Cryptococcus neoformans Grown In Vivo as Observed by Freeze-Etching

Respiratory activity is essential for post-exponential-phase production of type 5 capsular polysaccharide by Staphylococcus aureus

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