Aerodynamically assisted jet processing of viscous single- and multi-phase media

Arumuganathar, Sumathy; Jayasinghe, Suwan N.; Suter, Nicolai

doi:10.1039/b610554h

Cited by 21 publications

(18 citation statements)

References 26 publications

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“…Alginate microparticles were produced by ionic gelation using an aerodynamically assisted jetting system for the dispersion of microparticles [ 30 ]. The polymeric solution was pumped at 125 mL/h using a syringe pump (NE 8000, New Era Pump Systems Inc., Farmingdale, NY, USA), and the microparticles were stripped from the nozzle (XAPR 200A 303 SS, BETE Fog Nozzle, Inc, Greenfield, MA, USA) with the aid of a compressed air stream (1.25 bar), and dripped into a crosslinking solution (500 mL of CaCl 2 , 0.25 M, Sigma–Aldrich, St. Louis, MO, USA) from a height of 20 cm.…”

Section: Methodsmentioning

confidence: 99%

Assessing the Immune Response of Atlantic Salmon (Salmo salar) after the Oral Intake of Alginate-Encapsulated Piscirickettsia salmonis Antigens

Sotomayor-Gerding

Troncoso²,

Pino³

et al. 2020

Vaccines

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Salmon rickettsial septicaemia (SRS) is the infectious disease that produces the highest losses in the Chilean salmon industry. As a new strategy for the control of SRS outbreaks, in this study we evaluated the effect of alginate-encapsulated Piscirickettsia salmonis antigens (AEPSA) incorporated in the feed as an oral vaccine to induce the immune response in Atlantic salmon (Salmo salar). Fish were distributed into three vaccination groups (injectable, oral high dose, oral low dose). Feed intake and fish growth were recorded during the trial. The P. salmonis-specific IgM levels in blood plasma were measured by ELISA. Alginate microparticles containing the antigen were effectively incorporated in fish feed to produce the oral vaccine. Incorporation of AEPSA did not affect the palatability of the feed or the fish appetite. Furthermore, the oral vaccine did not have a negative effect on fish growth. Finally, the oral vaccine (high and low dose) produced an acquired immune response (IgM) similar to the injectable vaccine, generating a statistically significant increase in the IgM levels at 840-degree days for both experimental groups. These findings suggest that AEPSA incorporated in the feed can be an effective alternative to boost the immune response in Atlantic salmon (S. salar).

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Section: Methodsmentioning

confidence: 99%

Assessing the Immune Response of Atlantic Salmon (Salmo salar) after the Oral Intake of Alginate-Encapsulated Piscirickettsia salmonis Antigens

Sotomayor-Gerding

Troncoso²,

Pino³

et al. 2020

Vaccines

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show abstract

“…Prepolymers can be injected manually, 13,14 gravitationally, 15 using a syringe pump, 16,17 or using pressurized air. [18][19][20] To achieve chemically and mechanically stable fibers, the pre-polymer and the final polymer should be insoluble in the coagulation solution. 16 Fibers fabricated by this method can be deposited randomly or in a predefined pattern in the coagulation bath to form a porous scaffold or can be collected on a motorized spool and assembled in a consequent process.…”

Section: Wetspinningmentioning

confidence: 99%

Microtechnologies in the Fabrication of Fibers for Tissue Engineering

Akbari

Tamayol

Annabi

et al. 2014

Microfluidics for Medical Applications

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Engineering tissues and organs for implantation in the human body or research require the fabrication of constructs that reproduce a physiological environment. Moreover, the construction of complex and sizable three-dimensional tissues requires a precise control over cell distribution and an effective vasculature network to supply oxygen and nutrients, and remove waste. Fiber-based tissue engineering that forms 3D structures using fibers can address many of these challenges, but depends on the quality of the fibers. Recent progresses in microtechnologies have enabled researchers to fabricate biocompatible fibers with advanced biochemical and physical properties, including cell-laden fibers that are pre-seeded with cells. In this chapter, we discuss fiber fabrication techniques including co-axial flow spinning, wetspinning, meltspinning, and electrospinning, which have leveraged microtechnologies to improve their performance. We compare the properties of the fibers fabricated with these methods and discuss their strengths and weaknesses in the context of tissue engineering.

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“…The coagulation bath must be either a poor solvent or a non-solvent with respect to the polymer (Puppi et al, 2011). The flow of polymer solution can be produced manually (Enea et al, 2011, Kato et al, 1989), or using syringe pumps (Puppi, Dinucci, 2011, Spinks et al, 2006), or by applying pressure (Arumuganathar and Jayasinghe, 2008, Arumuganathar et al, 2007, Jayasinghe and Suter, 2010). Wetspinning is a well-established technique that can produce fibers with a wide a range of diameters from ~30 to 600 μm (Lee et al, 2011b).…”

Section: Fiber Formation Techniquesmentioning

confidence: 99%

Fiber-based tissue engineering: Progress, challenges, and opportunities

Tamayol

Akbari

Annabi

et al. 2013

Biotechnology Advances

410

376

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Tissue engineering aims to improve the function of diseased or damaged organs by creating biological substitutes. To fabricate a functional tissue, the engineered construct should mimic the physiological environment including its structural, topographical, and mechanical properties. Moreover, the construct should facilitate nutrients and oxygen diffusion as well as removal of metabolic waste during tissue regeneration. In the last decade, fiber-based techniques such as weaving, knitting, braiding, as well as electrospinning, and direct writing have emerged as promising platforms for making 3D tissue constructs that can address the above mentioned challenges. Here, we critically review the techniques used to form cell-free and cell-laden fibers and to assemble them into scaffolds. We compare their mechanical properties, morphological features and biological activity. We discuss current challenges and future opportunities of fiber-based tissue engineering (FBTE) for use in research and clinical practice.

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Aerodynamically assisted jet processing of viscous single- and multi-phase media

Cited by 21 publications

References 26 publications

Assessing the Immune Response of Atlantic Salmon (Salmo salar) after the Oral Intake of Alginate-Encapsulated Piscirickettsia salmonis Antigens

Assessing the Immune Response of Atlantic Salmon (Salmo salar) after the Oral Intake of Alginate-Encapsulated Piscirickettsia salmonis Antigens

Microtechnologies in the Fabrication of Fibers for Tissue Engineering

Fiber-based tissue engineering: Progress, challenges, and opportunities

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