Aerogenic Pasteurellas and Pasteurella-like Organisms Isolated in Czechoslovakia

Aldová, E; Frederiksen, Wilhelm; Paucková, V; Absolonová, V.; Vladík, P; Lávičková, Miluše; Hausner, O; Vokoun, Petr

doi:10.1016/s0934-8840(11)80882-0

Cited by 8 publications

(3 citation statements)

References 11 publications

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“…Reactions observed were in accordance with those previously reported by Mannheim et al (1978), Frederiksen (1981), Aldová et al (1992) and Stahel et al (2009). Stahel et al (2009), however, reported on two atypical isolates.…”

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confidence: 92%

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Classification of organisms previously reported as the SP and Stewart–Letscher groups, with descriptions of Necropsobacter gen. nov. and of Necropsobacter rosorum sp. nov. for organisms of the SP group

Korczak

Bojesen

Kuhnert

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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To allow classification of bacteria previously reported as the SP group and the Stewart–Letscher group, 35 isolates from rodents (21), rabbits (eight), a dog and humans (five) were phenotypically and genotypically characterized. Comparison of partial rpoB sequences showed that 34 of the isolates were closely related, demonstrating at least 97.4 % similarity. 16S rRNA gene sequence comparison of 20 selected isolates confirmed the monophyly of the SP group and revealed 98.5 %–100 % similarity between isolates. A blast search using the 16S rRNA gene sequences showed that the highest similarity outside the SP group was 95.5 % to an unclassified rat isolate. The single strain, P625, representing the Stewart–Letscher group showed the highest 16S rRNA gene similarity (94.9–95.5 %) to members of the SP group. recN gene sequence analysis of 11 representative strains resulted in similarities of 97–100 % among the SP group strains, which showed 80 % sequence similarity to the Stewart–Letscher group strain. Sequence similarity values based on the recN gene, indicative for whole genome similarity, showed the SP group being clearly separated from established genera, whereas the Stewart–Letscher group strain was associated with the SP group. A new genus, Necropsobacter gen. nov., with only one species, Necropsobacter rosorum sp. nov., is proposed to include all members of the SP group. The new genus can be separated from existing genera of the family Pasteurellaceae by at least three phenotypic characters. The most characteristic properties of the new genus are that haemolysis is not observed on bovine blood agar, positive reactions are observed in the porphyrin test, acid is produced from (+)-l-arabinose, (+)-d-xylose, dulcitol, (+)-d-galactose, (+)-d-mannose, maltose and melibiose, and negative reactions are observed for symbiotic growth, urease, ornithine decarboxylase and indole. Previous publications have documented that both ubiquinones and demethylmenaquinone were produced by the proposed type strain of the new genus, Michel A/76T, and that the major polyamine of representative strains (type strain not included) of the genus is 1,3-diaminopropane, spermidine is present in moderate amounts and putrescine and spermine are detectable only in minor amounts. The major fatty acids of strain Michel A/76T are C14 : 0, C16 : 0, C16:1ω7c and summed feature C14 : 0 3-OH/iso-C16 : 1 I. This fatty acid profile is typical for members of the family Pasteurellaceae. The G+C content of DNA of strain Michel A/76T was estimated to be 52.5 mol% in a previous investigation. The type strain is P709T ( = Michel A/76T = CCUG 28028T = CIP 110147T = CCM 7802T).

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confidence: 92%

“…However, reference was not given to the SP group. Frederiksen (1981), Aldová et al (1992) and Lion et al (1995) reported on human isolates and suggested that attention should be paid to these organisms also in medical laboratories.…”

mentioning

confidence: 99%

Classification of organisms previously reported as the SP and Stewart–Letscher groups, with descriptions of Necropsobacter gen. nov. and of Necropsobacter rosorum sp. nov. for organisms of the SP group

Korczak

Bojesen

Kuhnert

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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“…These bacteria are potentially infectious for humans. Several cases of bacteremia and/or focal suppuration have been reported ( 1 – 4 ). However, it is likely that the pathogenic role of N. rosorum is underestimated, as it is consistently misidentified by current commercial identification systems.…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Necropsobacter rosorum Strain P709 ^T

et al. 2014

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Necropsobacter

Bisgaard¹,

Kuhnert²

2020

Bergey's Manual of Systematics of Archaea and Bacteria

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Ne.crop.so.bac'ter. Gr. masc. n. nekros , dead body, corpse; Gr. fem. n. opsis , appearance, view; N.L. masc. n. bacter , a rod, N.L. masc. n. Necropsobacter , a rod isolated from an autopsy. Proteobacteria / Gammaproteobacteria / Pasteurellales / Pasteurellaceae / Necropsobacter The genus Necropsobacter includes taxa previously reported as the SP group and Stewart–Letcher (SL) group based on phenotypic characteristics. The genus includes typical members of the family Pasteurellaceae with respect to cultural characteristics and colony morphology. Neither hemin (X‐factor) nor NAD (V‐factor) is required for growth. Hemolysis is not observed on bovine blood agar. Acid is produced from (+)‐ l ‐arabinose, (+)‐ d ‐galactose, (+)‐ d ‐mannose, and maltose. Gas is produced from glucose. Negative reactions are observed for urease, ornithine decarboxylase, and indole. The genus includes isolates from guinea pigs and other rodents, rabbits, a dog, and humans. The taxon formed a monophyletic group based on 16S rRNA and partial rpoB sequence analysis compared to other genera of the family. 16S rRNA gene sequence comparison showed at least 98.5% similarity between isolates of the SP group, and strain P625 (SL group) only showed a 16S rRNA similarity of 95.5% to the SP group. The genus consists of only one species Necropsobacter rosorum . Strain P625 of the SL group remained unclassified at species level. Ubiquinones and demethylmenaquinone are produced by the type strain of N. rosorum . DNA G + C content (whole‐genomic sequencing) (mol%) : 48.9. Type species : Necropsobacter rosorum Christensen et al. 2011 VP .

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Aerogenic Pasteurellas and Pasteurella-like Organisms Isolated in Czechoslovakia

Cited by 8 publications

References 11 publications

Classification of organisms previously reported as the SP and Stewart–Letscher groups, with descriptions of Necropsobacter gen. nov. and of Necropsobacter rosorum sp. nov. for organisms of the SP group

Classification of organisms previously reported as the SP and Stewart–Letscher groups, with descriptions of Necropsobacter gen. nov. and of Necropsobacter rosorum sp. nov. for organisms of the SP group

Draft Genome Sequence of Necropsobacter rosorum Strain P709 ^T

Necropsobacter

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Aerogenic Pasteurellas and Pasteurella-like Organisms Isolated in Czechoslovakia

Cited by 8 publications

References 11 publications

Classification of organisms previously reported as the SP and Stewart–Letscher groups, with descriptions of Necropsobacter gen. nov. and of Necropsobacter rosorum sp. nov. for organisms of the SP group

Classification of organisms previously reported as the SP and Stewart–Letscher groups, with descriptions of Necropsobacter gen. nov. and of Necropsobacter rosorum sp. nov. for organisms of the SP group

Draft Genome Sequence of Necropsobacter rosorum Strain P709 T

Necropsobacter

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Resources

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Draft Genome Sequence of Necropsobacter rosorum Strain P709 ^T