Aeromicrobium chenweiae sp. nov. and Aeromicrobium yanjiei sp. nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively

Abstract

Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively. The cells were aerobic, non-motile, Gram-stain- and catalase-positive, rod-shaped bacteria. The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species. The four isolates produced acid from l-rhamnose, d-xylose and cellobiose, but were unable to reduce nitrate. The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively. The digital DNA–DNA hybridization value between strains 592T and MF47T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species. The four strains’ genomes displayed less than 24.6 % DNA–DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T. The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant respiratory quinones were MK-9(H4) and MK-8(H4). The cell-wall peptidoglycan contained ll-diaminopimelic acid. Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp. nov. and Aeromicrobium yanjiei sp. nov. The type strains are 592T (=CGMCC1.16526T=DSM 106289T) and MF47T (=CGMCC 1.17444T=JCM 33790T), respectively.

Aeromicrobium stalagmiti sp. nov., isolated from a lava cave

“…On the other hand, the only dDDH value was shown to be 32.5 % in an A. chenweiae–A. yanjiei pair in a previous work [2]. In this study, 16S rRNA gene sequence similarity and ANI values between these two type strains were 99.5 and 87.2 %, respectively.…”

“…chenweiae DSM 106289 T showed negative results for acid production from l -arabinose, cellobiose, d -lyxose, d -mannitol and d -xylose, and A. ginsengisoli KCTC 19207 T showed negative results for acid production from d -lyxose and d -mannitol, in contrast to those reported previously [2]. Strain YC3-14 T was differentiated from both A.…”

“…chenweiae DSM 106289 T (summed features 3 and 9) and A. ginsengisoli KCTC 19207 T (C 18 : 1 ω 9 c ) showed considerable differences in relative proportion from those reported previously [2]. Also, 10-methyl C 17 : 0 instead of 10-methyl C 16 : 0 in A.…”

“…The genus Aeromicrobium was proposed by Miller et al . [1] with Aeromicrobium erythreum as the type species for encompassing an aerobic, Gram-positive, motile actinomycete with coccoid or rod-shaped morphology and the genus now contains 21 species with validly published names (https://lpsn.dsmz.de/search?word=Aeromicrobium) including recently described species, Aeromicrobium chenweinae [2], Aeromicrobium endophyticum [3], Aeromicrobium phragmitis [4, 5], Aeromicrobium piscarium [6], Aeromicrobium terrae [7] and Aeromicrobium yanjiei [2]. Phylogenetically, this genus belongs to the family Nocardioidaceae [8] with members of genera Marmoricola and Nocardioides [9, 10].…”

Mycolicibacterium baixiangningiae sp. nov. and Mycolicibacterium mengxianglii sp. nov., two new rapidly growing mycobacterial species

“…Among quite a few novel isolates recovered from faecal samples of Tibetan antelope (TA), plateau pika and Himalayan marmot in our laboratory, Aeromicrobium chenweiae [17], Actinomyces wuliandei [18], Corynebacterium liangguodongii [18], Corynebacterium yudongzhengii [18], Oceanobacillus zhaokaii [18] and Cellulomonas shaoxiangyii [19], all isolated from TA, were validly published in 2020. In a continuation of our studies on the microbial diversity of endemic animals on the Qinghai-Tibetan Plateau, four new strains, also isolated from the TA faeces, were subjected to further taxonomic identification.…”