Aeromonadaceae Representative (Aeromonas salmonicida)

Austin, Brian; Austin, Dawn A.

doi:10.1007/978-94-007-4884-2_5

Cited by 30 publications

(38 citation statements)

References 367 publications

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“…The fish were transferred alive in tanks containing pond water to the microbiological lab in the Fish Diseases Department, Central Lab for aquaculture Research in Abbassa, Sharkia. All collected samples were subjected to clinical, postmortem and bacteriological examinations as described by Austin and Austin, (2012) and Noga, (2010).…”

Section: Materials and Methods Naturally Infected Fishmentioning

confidence: 99%

“…Under complete aseptic conditions, the specimens of different tissues and organs (skin ulcer, tail, gills, liver, kidneys, and spleen) from diseased fish were inoculated into Tryptic soy broth (Difco) and incubated at 27 o C for 24 h. Then loopful of broth were streaked onto Tryptic soy agar (TSA) plates and TSA plates with 2% NaCl and incubated at 27±1 o C for 48 h. For purification purposes, pure colonies were further sub-cultured into Tryptic soy agar. Each type of culture colony was picked up and sub-cultured on a selective diagnostic agar media (Thiosulphate citrate bile salt agar (Oxoid) (TCBS), Aeromonas base agar media supplemented with ampicillin (5 mg/L) and Pseudomonas base agar at 27ºC for 48 h. Then pure colonies were transferred onto TSA slant for further identification that carried out according to Austin andAustin, (2012) andQuinn et al, (2002).…”

Section: Bacterial Isolationmentioning

confidence: 99%

“…The obtained bacterial isolates were subjected to full identification by using colonies characteristics, Gram stain, and biochemical activities as previously described (Austin and Austin, 2012;Quinn et al, 2002).…”

Section: Bacterial Identificationmentioning

confidence: 99%

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Isolation, phenotypic characterization and antibiotic susceptibility of prevalent bacterial pathogens implicating the mortality of cultured Nile tilapia, Oreochromis niloticus

Hassan

Abdel-Rahman

Mansour

et al. 2020

Egyptian Journal for Aquaculture

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Nile-Tilapia (Oreochromis niloticus) aquaculture represents one of the most important cultivation species in Egypt. However, Tilapia fish farming is challenged by some problems. Of those, the presence of bacterial pathogens resulting in high fish mortalities and huge economic losses. Thus, the current investigation aimed to isolate, identify, and characterize the pathogenic bacteria from Nile tilapia fish farm in El-Abassa village, Egypt and to investigate their antibiotic susceptibility as a primary step for controlling diseases. 182 bacterial isolates were obtained from one hundred Tilapia fish samples. The microbiological and biochemical analysis of the examined fish indicated the presence of only 5 bacterial genera. Three of them are Gram-negative bacteria (representing 86.26% of total isolates) including Aeromonas spp. (46.70 %), Pseudomonas spp. (23.08 %), and Vibrio spp. (16.48 %). While two genera are Gram-positive bacteria (representing 13.74% of the total isolates) including Streptococcus spp. (8.79 %) and Staphylococcus spp. (4.95 %). This indicates that Gramnegative bacteria are the main cause of high fish mortalities in the studied area while Aeromonas hydrophila exhibited the highest prevalence in infected tilapia. Antibiogram test revealed high levels of resistance expressed by all isolates to ampicillin, amoxicillin, and erythromycin. On the other hand, norfloxacin was effective against all isolated bacteria followed by ciprofloxacin; therefore, norfloxacin should be 24recommended as a supplement in fish fed-diets to control the bacterial infection. Establishing effective control methods for pathogenic isolates would greatly enhance fish production.

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Section: Materials and Methods Naturally Infected Fishmentioning

confidence: 99%

Section: Bacterial Isolationmentioning

confidence: 99%

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Isolation, phenotypic characterization and antibiotic susceptibility of prevalent bacterial pathogens implicating the mortality of cultured Nile tilapia, Oreochromis niloticus

Hassan

Abdel-Rahman

Mansour

et al. 2020

Egyptian Journal for Aquaculture

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“…As an example, the species Aer. salmonicida has been described as the cause of massive mortality and great economic loss in marine and continental aquaculture, particularly affecting salmonids (Austin and Austin 1993;Hiney et al 1994;O'Brien et al 1994;Goldschmidt-Clermont et al 2009). Some other fish species like turbot (Scopthalmus maximus), Atlantic cod (Gadus morhua), halibut (Hippoglossus hippoglossus), carp (Cyprinus carpio) and goldfish (Carassius auratus) were also affected (Wiklund and Dalsgaard 1998).…”

Section: Introductionmentioning

confidence: 99%

Identity and virulence properties of Aeromonas isolates from diseased fish, healthy controls and water environment in China

Wang

Pan

et al. 2012

Letters in Applied Microbiology

113

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Aims: To investigate the species distribution in Aeromonas isolates from diseased fish, healthy controls and water environment in China; to evaluate the frequency of the aerolysin (aer), cytotonic enterotoxin (alt), cytotoxic enterotoxin (act), temperature‐sensitive protease (eprCAI) and serine protease (ahp) genes in Aeromonas isolates; and to determine the potential pathogenicity of these isolates. Methods and Results: Two hundred and two Aeromonas isolates from diseased fish (n = 42), healthy fish (n = 120) and water environment (n = 40) in China were identified to species levels based on sequencing of the housekeeping gene gyrB, while the distribution of five virulence factors, including aer, alt, act, eprCAI and ahp, was investigated by PCR. Aeromonas veronii (25/42; 60%) and Aeromonas hydrophila (14/42; 33%) were the species most commonly isolated from diseased fish, while Aer. veronii was the most common species in healthy fish (90/120; 75%) and water samples (25/40; 62·5%). All the five virulence genes were present in 9% (19/202), among which 10 strains were from diseased fish and nine were identified as Aer. hydrophila. For the strains carrying five virulence genes, the average 50% lethal doses (LD50s) of strains from diseased fish were lower when compared with the strains from healthy fish and water environment. Conclusions: Aeromonas veronii is the most common species, but no significant difference exists in the isolates obtained from diseased fish and from healthy fish. However, Aer. hydrophila isolates were significantly more frequent from diseased fish than from healthy fish. aer+alt+act+eprCAI+ahp+ was more frequent virulence genotype in Aeromonas isolates from diseased fish than from healthy fish and water environment, and the aer+alt+act+eprCAI+ahp+ isolates were more virulent to zebrafish comparing to the other genetic profiles. Significant and Impact of the Study: Aeromonas species in aquatic environments are various and have considerable virulence potential, and therefore, there is a need for more careful and intensive epidemiology studies.

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“…These outbreaks have substantially reduced its production, coupled with other health concerns. In most of the cases, bacteria are frequently isolated during a fish disease outbreak (Austin & Austin, 2012).…”

Section: Introductionmentioning

confidence: 99%

Specific immune response in mucosal and systemic compartments of Cirrhinus mrigala vaccinated against Edwardsiella tarda: In vivo kinetics using different antigen delivery routes

Qadiri

Makesh

Rajendran

et al. 2018

J World Aquaculture Soc

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Mucosal immune barriers confer protection against invading fish pathogens. Here, we conducted an experiment for 60 days to assess the mucosal and systemic immune response in Mrigal (Cirrhinus mrigala), an Indian major carp. Fish were immunized with inactivated Edwardsiella tarda by four different routes, namely, oral, immersion, injection, and anal intubation. An indirect enzyme‐linked immunosorbent assay (ELISA) was used to measure the specific immune response (antibody) in serum and mucus (collected from skin, gill, and gut) of the fish on 0, 15, 30, 45, and 60 days postimmunization. For specific immune response in the serum, significantly higher (p < 0.05) optical density (OD) values were obtained in the anal group (0.52 ± 0.03) and in the oral group (0.48 ± 0.03). In the skin mucus, significantly higher OD values were obtained in the oral group (0.48 ± 0.04) and immersion group (0.32 ± 0.03). In the gill mucus, significantly higher OD values were obtained in the oral group (0.82 ± 0.08) and the immersion group (0.73 ± 0.03). In the gut mucus, significantly higher OD values were obtained in the immersion group (0.080 ± 0.007) compared to the rest of the treatments. Fish from all the groups were challenged with LD50 dose of E. tarda at the end of the experiment. We conclude that oral and immersion immunization routes offer better protection of C. mrigala compared to other antigen delivery routes.

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Aeromonadaceae Representative (Aeromonas salmonicida)

Cited by 30 publications

References 367 publications

Isolation, phenotypic characterization and antibiotic susceptibility of prevalent bacterial pathogens implicating the mortality of cultured Nile tilapia, Oreochromis niloticus

Isolation, phenotypic characterization and antibiotic susceptibility of prevalent bacterial pathogens implicating the mortality of cultured Nile tilapia, Oreochromis niloticus

Identity and virulence properties of Aeromonas isolates from diseased fish, healthy controls and water environment in China

Specific immune response in mucosal and systemic compartments of Cirrhinus mrigala vaccinated against Edwardsiella tarda: In vivo kinetics using different antigen delivery routes

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