Aeromonadaceae Representative (Aeromonas salmonicida)

Austin, Brian; Austin, Dawn A.

doi:10.1007/978-3-319-32674-0_5

Cited by 42 publications

(41 citation statements)

References 390 publications

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“…Aeromonas hydrophila, a Gram-negative rod-shaped bacterium, is the main cause of the disease motile Aeromonas septicemia, also known as tail and fin rot. This bacterium causes serious infections in various freshwater fish species, including loach (Misgurnus anguillicaudatus), channel catfish (Ictalurus punctatus), and common carp (Cyprinus carpio), and infects some marine fish species to a lesser extent [4]. Clinical signs of these infections include 2 of 13 ulcers, abdominal distension, accumulation of fluid, anemia, and hemorrhaging, resulting in mass mortality in fishes around the world [4,5].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization, and Application of a Bacteriophage Infecting the Fish Pathogen Aeromonas hydrophila

et al. 2020

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Bacteriophages are increasingly being used as biological control agents against pathogenic bacteria. In the present study, we isolate and characterize bacteriophage Akh-2 from Geoje Island, South Korea, to evaluate its utility in controlling motile Aeromonas septicemia. Akh-2 lysed four of the seven Aeromonas hydrophila strains tested. Transmission electron microscopy analysis showed that Akh-2 belongs to the Siphoviridae family, with head and tail sizes of 50 ± 5 and 170 ± 5 nm, respectively. One-step growth curve analysis revealed that the phage has a latent period of 50 ± 5 min and a burst size of 139 ± 5 plaque-forming units per infected cell. The phage appeared stable in a pH range of 6–8 and a temperature range of −80 to 46 °C. Based on next-generation sequencing analysis, its genome is 114,901 bp in size, with a 44.22% G + C content and 254 open reading frames. During an artificial induction of the disease, loach (Misgurnus anguillicaudatus) treated with Akh-2 showed an increased survival rate and time compared with the non-treated control. Our results suggest that Akh-2 is a potential biological agent for the treatment of Aeromonas infections in fish.

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Section: Introductionmentioning

confidence: 99%

Isolation, Characterization, and Application of a Bacteriophage Infecting the Fish Pathogen Aeromonas hydrophila

et al. 2020

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“…), which have an unclear taxonomy and no subspecies assignment yet. This bacterium is of veterinary importance as many taxa belonging to the different subspecies are known to infect fish (Burr & Frey ; Austin & Austin ), thus causing important livestock losses worldwide (Dallaire‐Dufresne et al . ).…”

Section: Aeromonas Salmonicida Isolates Used In This Studymentioning

confidence: 99%

“…). The most studied subspecies of this bacterium is salmonicida , which is sometimes qualified as ‘typical’, and is well known to cause furunculosis, a fish disease mainly affecting salmonids (Austin & Austin ). The other psychrophilic subspecies are qualified as ‘atypical’ and infect a wide range of hosts (Dallaire‐Dufresne et al .…”

Section: Aeromonas Salmonicida Isolates Used In This Studymentioning

confidence: 99%

Phylogenetic analysis of the fish pathogen Aeromonas salmonicida underlines the dichotomy between European and Canadian strains for the salmonicida subspecies

Vincent

Charette

2017

Journal of Fish Diseases

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“…This species is divided into five officially recognized subspecies: salmonicida, smithia, achromogenes, masoucida, and pectinolytica [1]. Strains of the subspecies salmonicida, smithia, achromogenes and masoucida can infect a wide range of fish and are psychrophilic with their growth being limited to temperatures not higher than about 25 • C [1][2][3]. In 2000, the pectinolytica subspecies was isolated directly from a polluted river (Matanza River, Argentina) without having any known host [4].…”

Section: Introductionmentioning

confidence: 99%

A Mesophilic Aeromonas salmonicida Strain Isolated from an Unsuspected Host, the Migratory Bird Pied Avocet

et al. 2019

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Aeromonas salmonicida is a Gram-negative bacterium, known as a fish pathogen since its discovery. Although the species was initially considered psychrophilic, a mesophilic subspecies (pectinolytica) and many other mesophilic strains still not attributed to subspecies have been described in the last two decades. These mesophilic strains were sampled from various sources, including humans, and some of them are known to be pathogenic. In this study, we describe a strain, JF2480, which was isolated from the spleen, and also found the kidney and liver of a dead pied avocet (Recurvirostra avosetta), a type of migratory bird inhabiting aquatic environments. A core genome phylogenomic analysis suggests that JF2480 is taxonomically distant from other known A. salmonicida subspecies. The genome sequence confirms that the strain possesses key virulence genes that are present in the typical A. salmonicida psychrophilic subspecies, with the exception of the genes encoding the type three secretion system (T3SS). Bacterial virulence assays conducted on the surrogate host Dictyostelium discoideum amoeba confirmed that the strain is virulent despite the lack of T3SS. Bacterial growth curves showed that strain JF2480 grow well at 40 °C, the body temperature of the pied avocet, and even faster at 41 °C, compared to other mesophilic strains. Discovery of this strain further demonstrates the extent of the phylogenomic tree of this species. This study also suggests that A. salmonicida can infect a wider array of hosts than previously suspected and that we need to rethink the way we perceive A. salmonicida’s natural environment.

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Aeromonadaceae Representative (Aeromonas salmonicida)

Cited by 42 publications

References 390 publications

Isolation, Characterization, and Application of a Bacteriophage Infecting the Fish Pathogen Aeromonas hydrophila

Isolation, Characterization, and Application of a Bacteriophage Infecting the Fish Pathogen Aeromonas hydrophila

Phylogenetic analysis of the fish pathogen Aeromonas salmonicida underlines the dichotomy between European and Canadian strains for the salmonicida subspecies

A Mesophilic Aeromonas salmonicida Strain Isolated from an Unsuspected Host, the Migratory Bird Pied Avocet

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