Aeromonas detection and their toxins from drinking water from reservoirs and drinking fountains

Razzolini, María Tereza Pepe; Bari, Marisa Di; Sanchez, Petra Sanchez; Sato, Maria Inês Zanoli

doi:10.2166/wh.2007.018

Cited by 24 publications

(17 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aeromonas has been isolated from clinical samples as the sole pathogen, where it was inferred to be the cause of diarrhoea in the studied patients (Hofer et al 2006). Previous studies have shown that Aeromonas strains can carry virulence determinants responsible for infection in humans (Kingombe et al 1999;Sen & Rodgers 2004;Razzolini et al 2008). veronii, Aeromonas schubertii and Aeromonas trota have also been isolated from clinical samples (Ormen et al 2003).…”

Section: Introductionmentioning

confidence: 90%

Molecular detection of enterotoxins in environmental strains of Aeromonas hydrophila and Aeromonas jandaei

Balsalobre

Dropa

Matté

et al. 2009

Journal of Water and Health

View full text Add to dashboard Cite

Aeromonas species are widely distributed in aquatic environments and recent studies include the genus in the emergent pathogens group because of its frequent association with local and systemic infections in immunocompetent humans. Aiming to search for virulence genes in environmental strains of Aeromonas hydrophila and Aeromonas jandaei, we designed specific primers to detect act/hly A/aer complex and alt genes. Primers described elsewhere were used to detect ast. Eighty-seven strains previously identified using phenotypic and genotypic tests as A. hydrophila (41) and A. jandaei (46) were analysed for the presence of the virulence genes using PCR. DNA fragments of expected size were purified and directly sequenced. Among the 41 strains of A. hydrophila 70.7% (29), 97.6% (40) and 26.8% (11) possessed act/hly A/aer complex, ast and alt genes, respectively. Among the 46 strains of A. jandaei, 4.4% (2), 0% (0) and 32.6% (15) were positive for act/hly A/aer complex, ast and alt genes, respectively. Sequencing allowed for the confirmation of amplified products using BLAST. The present work proposes a specific and rapid diagnostic method to detect the main virulence determinants of Aeromonas, a genus potentially pathogenic to humans.

show abstract

Section: Introductionmentioning

confidence: 90%

Molecular detection of enterotoxins in environmental strains of Aeromonas hydrophila and Aeromonas jandaei

Balsalobre

Dropa

Matté

et al. 2009

Journal of Water and Health

View full text Add to dashboard Cite

show abstract

“…hydrophila has also been found in drinking water in (19). These results highlight the pathogenic potential of…”

Section: Study Areamentioning

confidence: 54%

“…Thus, some authors consider that these organisms should be a sanitary quality indicator (7,19,21,24). Moreover, Aeromonas hydrophila is listed in the first and second Contaminant Candidate List (CCL1 and CCL2) by the US Environmental Protection Agency -USEPA (26), linking these organisms to public health adverse outcomes.…”

Section: Study Areamentioning

confidence: 99%

“…Aeromonas genus is considered an emerging pathogen (26,27) and responsible for gastrointestinal disturbances (19), septicemia, endocarditis, conjunctivitis and wound infections (8,9,13,16,18). These organisms are largely distributed in several water sources such as rivers, lakes, wells, sewage, wastewaters and drinking water supplies (19,24).…”

Section: Introductionmentioning

confidence: 99%

“…These organisms are largely distributed in several water sources such as rivers, lakes, wells, sewage, wastewaters and drinking water supplies (19,24).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Aeromonas presence in drinking water from collective reservoirs and wells in peri-urban area in Brazil

Razzolini¹,

Günther²,

Martone-Rocha³

et al. 2010

Braz. J. Microbiol.

Self Cite

View full text Add to dashboard Cite

Aeromonas genus is considered an emerging pathogen and its presence in drinking water supplies is a reason to public health concern. This study investigated the occurrence of Aeromonas in samples from collective reservoirs and wells used as drinking water sources in a peri-urban area. A total of 35 water samples were collected from collective reservoirs and 32 from wells bimonthly, from September 2007 to September 2008. Aeromonas spp determination was carried out using a Multiple-Tube Technique. Samples were inoculated into alkaline peptone water and the superficial film formed was transferred to blood agar plates amended with ampicillin. Typical Aeromonas colonies were submitted to a biochemical screening and then to biochemical tests for species differentiation. Aeromonas was detected in 13 (19%) of the 69 samples examined (6 from collective reservoirs and 7 from wells). Concentrations of Aeromonas in collective reservoirs ranged from <0.3 to 1.2 x10 2 MPN/100mL and, in wells, from <0.3 to 2.4 x10 2 MPN/100mL. The most frequent specie in the collective reservoir samples was Aeromonas spp (68%), followed by A. encheleia (14%) and A. allosaccharophila (8%) and A. hydrophila (8%). Aeromonas spp (87%) was the most frequent specie isolated from well samples, followed by A. allosacchariphila (8%), A. encheleia (2%) and A. jandaei (5%). These data show the presence and diversity of Aeromonas genus in the samples analyzed and highlight that its presence in drinking water poses a significant public health concern.

show abstract

Detection and distribution of putative virulence associated genes inAeromonasspecies from freshwater and wastewater treatment plant

Igbinosa

Okoh

2013

J. Basic Microbiol.

View full text Add to dashboard Cite

The detection of genes responsible for Aeromonas virulence is a vital tool in establishing the potential pathogenicity of the bacteria, as these virulence genes may act alone or in synergy in the establishment of infections. Freshwater and wastewater mixed liquor samples were collected from Kat river and Fort Beaufort wastewater treatment plant in the Eastern Cape Province of South Africa. Polymerase chain reaction was utilized for the amplification of the different genes coding for virulence. All virulence associated genes screened (alt, lip, fla, aer, ast, hlyA) were detected in at least one Aeromonas isolates. In fresh water sample, virulence genes were distributed as follows: lip (67%), aer (43%), alt (33%), fla (62%), ast (10%), and hlyA (86%), while in wastewater samples the occurrence were as follows: lip (92%), aer (21%), alt (54%), fla (83%), ast (29%), and hlyA (88%). The presence of these virulence genes in environmental Aeromonas isolates is of concern to public health as these organisms are potential pathogens in the environment and the virulence determinants could be transferred to aquatic organisms and humans by one mechanism or the other.

show abstract

Aeromonas detection and their toxins from drinking water from reservoirs and drinking fountains

Cited by 24 publications

References 27 publications

Molecular detection of enterotoxins in environmental strains of Aeromonas hydrophila and Aeromonas jandaei

Molecular detection of enterotoxins in environmental strains of Aeromonas hydrophila and Aeromonas jandaei

Aeromonas presence in drinking water from collective reservoirs and wells in peri-urban area in Brazil

Detection and distribution of putative virulence associated genes inAeromonasspecies from freshwater and wastewater treatment plant

Contact Info

Product

Resources

About