Aeromonas hydrophila induced head kidney macrophage apoptosis in Clarias batrachus involves the activation of calpain and is caspase-3 mediated

Banerjee, Chaitali; Goswami, Ramansu; Verma, Gaurav; Datta, Malabika; Mazumder, Shibnath

doi:10.1016/j.dci.2012.02.005

Cited by 39 publications

(38 citation statements)

References 46 publications

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“…Therefore, to examine whether increased ROS and RNS generation observed earlier is a prerequisite to apoptosis production in CTM cells, the apoptotic population by flow cytometry using Annexin-V/PI assay was ascertained. A. hydrophila mediated apoptosis has been earlier reported in murine macrophages and head kidney macrophages of fish (Mazumdar et al, 2004;Banerjee et al, 2012;Banerjee et al, 2014a). However, there are no existing reports of the apoptosis induction in the thymic macrophages in response to A. hydrophila challenge.…”

Section: Discussionmentioning

confidence: 95%

Lactobacillus acidophilus attenuates Aeromonas hydrophila induced cytotoxicity in catla thymus macrophages by modulating oxidative stress and inflammation

Patel

Kumar

Banerjee

et al. 2016

Molecular Immunology

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Section: Discussionmentioning

confidence: 95%

Lactobacillus acidophilus attenuates Aeromonas hydrophila induced cytotoxicity in catla thymus macrophages by modulating oxidative stress and inflammation

Patel

Kumar

Banerjee

et al. 2016

Molecular Immunology

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“…However, A. hydrophila -induced pathogenicity is not well understood. Macrophages are essential for innate immune responses against A. hydrophila and apoptosis has been extensively implicated in macrophage- A. hydrophila interactions34. However, it is difficult to predict whether the outcomes are to the advantage of the host or the pathogen5.…”

mentioning

confidence: 99%

“…A. hydrophila -induced macrophage apoptosis is executed by an intracellular proteolytic cascade of caspases, where initiator caspases are triggered by a range of intracellular signalling molecules to accomplish programmed demolition of cell through caspase-3 activation346. As an early initiator of the process, the role of calcium (Ca 2+ ) is foremost7.…”

mentioning

confidence: 99%

Ameliorating ER-stress attenuates Aeromonas hydrophila-induced mitochondrial dysfunctioning and caspase mediated HKM apoptosis in Clarias batrachus

Banerjee

Singh

Das

et al. 2014

Sci Rep

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Endoplasmic reticulum (ER)-stress and unfolding protein response (UPR) has not been implied in Aeromonas hydrophila-pathogenicity. We report increased expression of the ER-stress markers: CHOP, BiP and phospho-eIF2α in A. hydrophila-infected headkidney macrophages (HKM) in Clarias batrachus. Pre-treatment with ER-stress inhibitor, 4-PBA alleviated ER-stress and HKM apoptosis suggesting ER-UPR critical for the process. The ER-Ca2+ released via inositol-triphosphate and ryanodine receptors induced calpain-2 mediated superoxide ion generation and consequent NF-κB activation. Inhibiting NF-κB activation attenuated NO production suggesting the pro-apoptotic role of NF-κB on HKM pathology. Calpain-2 activated caspase-12 to intensify the apoptotic cascade through mitochondrial-membrane potential (ψm) dissipation and caspase-9 activation. Altered mitochondrial ultra-structure consequent to ER-Ca2+ uptake via uniporters reduced ψm and released cytochrome C. Nitric oxide induced the cGMP/PKG-dependent activation of caspase-8 and truncated-Bid formation. Both the caspases converge onto caspase-3 to execute HKM apoptosis. These findings offer a possible molecular explanation for A. hydrophila pathogenicity.

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“…The reason may be that PfBCL-2 directly participated in cell apoptosis. It has been reported that A. hydrophila induces apoptosis in fish (Shao et al 2004;Banerjee et al 2012). Apoptosis appears to be mediated by several different mechanisms, such as phosphorylation of BCL-2 and Bcl-Xl, the proportion of BCL-2/Bax (Han et al 2013).…”

Section: Discussionmentioning

confidence: 98%

Identification, characterization and functional analysis of anti-apoptotic protein BCL-2-like gene from pufferfish, Takifugu obscurus, responding to bacterial challenge

Cheng

Liao

Miao

et al. 2015

Fish Physiol Biochem

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Apoptosis plays a crucial role in many biological processes, including development, cellular homeostasis and immune responses. The BCL-2 family is a key regulator of the mitochondrial response to apoptotic signals in the intrinsic pathway. In this study, we identified and characterized the cDNA and expression pattern of pufferfish BCL-2 (PfBCL-2). The full-length cDNA of PfBCL-2 was 1412 bp with an open reading frame of 657 bp encoding a putative protein of 219 amino acids (Accession no: KP898414). The calculated molecular mass of the PfBCL-2 was 24.2 kDa with a predicted isoelectric point of 5.27. The deduced PfBCL-2 protein exhibited four highly conserved BCL-2 homology domains, suggesting that PfBCL-2 may play a similar role in the apoptotic-signaling pathway as in other species. Real-time PCR results showed that PfBCL-2 transcript was expressed in a wide range of tissues but exhibited the greatest level of expression in blood. Transcriptional responses of PfBCL-2 exhibited different spatial and temporal expression profiles in liver and blood after bacterial infection. PfBcl-2 transcript was significantly up-regulated in liver at 6, 12, 24 and 48 h (with maximum induction at 48 h) and was up-regulated in blood at 3, 6, 12 and 24 h (with maximum induction at 12 h). Meanwhile, recombinant PfBCL-2 fused with His6 tag was efficiently expressed in Escherichia coli BL21 (DE3) and purified using Ni-nitrilotriacetic acid resin. Western blot analysis indicated that its protein level appeared to be elevated during the initial bacterial infection. These results suggest that PfBCL-2 plays important roles in immune responses against bacteria challenge.

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Aeromonas hydrophila induced head kidney macrophage apoptosis in Clarias batrachus involves the activation of calpain and is caspase-3 mediated

Cited by 39 publications

References 46 publications

Lactobacillus acidophilus attenuates Aeromonas hydrophila induced cytotoxicity in catla thymus macrophages by modulating oxidative stress and inflammation

Lactobacillus acidophilus attenuates Aeromonas hydrophila induced cytotoxicity in catla thymus macrophages by modulating oxidative stress and inflammation

Ameliorating ER-stress attenuates Aeromonas hydrophila-induced mitochondrial dysfunctioning and caspase mediated HKM apoptosis in Clarias batrachus

Identification, characterization and functional analysis of anti-apoptotic protein BCL-2-like gene from pufferfish, Takifugu obscurus, responding to bacterial challenge

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