Aeromonas hydrophila infection activates death receptor apoptosis pathway in the red blood cells of grass carp (Ctenopharyngodon idellus)

Lu, Zhijie; Yang, Minxuan; Zhang, Kai; Li, Fenglin; Shi, Fei; Li, Yanan; Zhao, Lijuan; Li, Jun; Lin, Li; Qin, Zhendong

doi:10.1016/j.aquaculture.2020.735956

Cited by 19 publications

(3 citation statements)

References 41 publications

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“…Accompanied with this, both the protein level of caspase-3 and the mRNA expression of related genes including caspase-3 , caspase-9 , apaf-1 , p53 and baxa also significantly increased after infection. This was in accordance with previous studies in grass carp, which also detected the increased apoptosis in gill during F. columnare infection [ 73 ] and in the red blood cells during Aeromonas hydrophila infection [ 88 ]. In this study, dietary glutamine supplementation significantly reduced the ratio of apoptotic cells in the gill after bacterial infection, indicated by the TUNEL assay.…”

Section: Discussionsupporting

confidence: 93%

Dietary Glutamine Inclusion Regulates Immune and Antioxidant System, as Well as Programmed Cell Death in Fish to Protect against Flavobacterium columnare Infection

et al. 2021

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The susceptibility of animals to pathogenic infection is significantly affected by nutritional status. The present study took yellow catfish (Pelteobagrus fulvidraco) as a model to test the hypothesis that the protective roles of glutamine during bacterial infection are largely related to its regulation on the immune and antioxidant system, apoptosis and autophagy. Dietary glutamine supplementation significantly improved fish growth performance and feed utilization. After a challenge with Flavobacterium columnare, glutamine supplementation promoted il-8 and il-1β expression via NF-κB signaling in the head kidney and spleen, but inhibited the over-inflammation in the gut and gills. Additionally, dietary glutamine inclusion also enhanced the systematic antioxidant capacity. Histological analysis showed the protective role of glutamine in gill structures. Further study indicated that glutamine alleviated apoptosis during bacterial infection, along with the reduced protein levels of caspase-3 and the reduced expression of apoptosis-related genes. Moreover, glutamine also showed an inhibitory role in autophagy which was due to the increased activation of the mTOR signaling pathway. Thus, our study for the first time illustrated the regulatory roles of glutamine in the fish immune and antioxidant system, and reported its inhibitory effects on fish apoptosis and autophagy during bacterial infection.

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Section: Discussionsupporting

confidence: 93%

Dietary Glutamine Inclusion Regulates Immune and Antioxidant System, as Well as Programmed Cell Death in Fish to Protect against Flavobacterium columnare Infection

et al. 2021

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“…In the Atlantic salmon, erythrocytes infected with the infectious salmon anaemia virus revealed virus‐induced haemagglutination and IFN‐α induction (Puente‐Marin et al ., 2019a; Workenhe et al ., 2008). In the Japanese flounder, RBCs differentially expressed many genes related to TLRs and NLRs against E. tarda infections (Sun et al ., 2020), and in the grass carp, RBCs expressed CCL4, CCL11, CCL20, IL‐4, IL‐12 and IFN‐α in response to A. hydrophila infection (Lu et al ., 2021). As observed in these fish species, we speculated that there is a similar important role of RBCs in the Indian major carp, rohu.…”

Section: Discussionmentioning

confidence: 99%

Red blood cells of Labeo rohita express Toll‐like receptors, NOD‐ like receptors, interleukins, and interferon‐I in response to Gram‐negative bacterial infections and lipopolysaccharide stimulations

Mahapatra

Ganguly

Pani

et al. 2023

Journal of Fish Biology

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Red blood cells (RBCs) are the most abundant cell types in the circulatory system of vertebrates. In fish, RBCs retain their nuclei throughout their lifetime and remain transcriptionally and translationally active. While their primary function is typically associated with gas exchange, recent reports indicate that nucleated red blood cells can play a significant role in regulating the body's innate immune response. The current article describes the innate immune role of red blood cells in rohu (Labeo rohita), a freshwater fish species that holds significant commercial importance in India and South‐East Asian nations. From the whole blood and mucosal surface RBCs have been isolated through density gradient centrifugation with HiSep™LSM 1077 (density 1.007 ± 0.0010) and their purity has been confirmed by the Giemsa staining followed by microscopical observations. Toll‐like receptors (TLR2, 3, 4, 5) and nucleotide oligomerization domain (NOD)‐like receptors (NOD1 and NOD2) in RBCs of rohu fingerlings were observed to be significantly activated (P < 0.05) on infection with Aeromonas hydrophila and Edwardsiella tarda. This activation resulted in increased expression of interleukins (IL‐8, IL‐1β) and interferon (IFN)‐I genes. The activation of TLR4, NOD1 and NOD2, as well as the expression of interleukins and IFN‐I genes have been observed in both in vivo and in vitro stimulation of rohu RBCs with lipopolysaccharides. These findings highlight the importance of fish RBCs in enhancing innate immunity against various pathogenic invasions in rohu.

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“…In Japanese flounder, cell apoptosis, along with the up-regulation of NLRP3, ASC, caspase-1, IL1β, and IL18, in the macrophages has been observed after Edwardsiella tarda infection [ 65 ]. In addition, the apoptosis of erythrocytes can be induced by A. hydrophila infection in grass carp, along with the up-regulation of CCL4, CCL11, CCL20, IL4, and IL12 [ 66 ]. These studies indicate that the inflammation caused by bacterial infection is often accompanied by cell apoptosis in hosts.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals the Molecular Immunopathogenesis of Chinese Soft-Shelled Turtle (Trionyx sinensis) Infected with Aeromonas hydrophila

Tan

et al. 2021

Biology

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Although hemorrhagic sepsis caused by Aeromonas hydrophila infection is the dominant disease in the aquaculture of Chinese soft-shelled turtle, information on its molecular pathology is seriously limited. In this study, ninety turtles intraperitoneally injected with A. hydrophila exhibited two different phenotypes based on the pathological symptoms, referred to as active and inactive turtles. Comparative transcriptomes of liver and spleen from these two groups at 6, 24, and 72 h post-injection (hpi) were further analyzed. The results showed that cytokine–cytokine receptor interaction, PRRs mediated signaling pathway, apoptosis, and phagocytosis enriched in active and inactive turtles were significantly different. Pro-inflammatory cytokines, the TLR signaling pathway, NLR signaling pathway, and RLR signaling pathway mediating cytokine expression, and apoptosis-related genes, were significantly up-regulated in inactive turtles at the early stage (6 hpi). The significant up-regulation of phagocytosis-related genes occurred at 24 hpi in inactive turtles and relatively lagged behind those in active turtles. The anti-inflammatory cytokine, IL10, was significantly up-regulated during the tested periods (6, 24, and 72 hpi) in active turtles. These findings offer valuable information for the understanding of molecular immunopathogenesis after A. hydrophila infection, and facilitate further investigations on strategies against hemorrhagic sepsis in Chinese soft-shelled turtle T. sinensis.

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Aeromonas hydrophila infection activates death receptor apoptosis pathway in the red blood cells of grass carp (Ctenopharyngodon idellus)

Cited by 19 publications

References 41 publications

Dietary Glutamine Inclusion Regulates Immune and Antioxidant System, as Well as Programmed Cell Death in Fish to Protect against Flavobacterium columnare Infection

Dietary Glutamine Inclusion Regulates Immune and Antioxidant System, as Well as Programmed Cell Death in Fish to Protect against Flavobacterium columnare Infection

Red blood cells of Labeo rohita express Toll‐like receptors, NOD‐ like receptors, interleukins, and interferon‐I in response to Gram‐negative bacterial infections and lipopolysaccharide stimulations

Comparative Transcriptome Analysis Reveals the Molecular Immunopathogenesis of Chinese Soft-Shelled Turtle (Trionyx sinensis) Infected with Aeromonas hydrophila

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