Aeromonas hydrophila motY is essential for polar flagellum function, and requires coordinate expression of motX and Pom proteins

Molero, Raquel; Wilhelms, Markus; Infanzón, Belén; Tomás, Juan M.; Merino, Susana

doi:10.1099/mic.0.049544-0

Cited by 17 publications

(20 citation statements)

References 44 publications

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“…This suggests that other strains of A. piscicola are also able to adhere to and invade fish cells. Two essential proteins (motY and motX) for the motility of the polar flagellum have also been recognized using mutant strains of AH3 (Molero et al 2011). Yeh & Klesius (2011) amplified and purified 19 A. hydrophila flagellar proteins and found that one of them, the FlgK protein (which is a flagellar hook-filament junction protein involved in bacterial motility, colonization and invasion) showed a strong reaction against the sera of infected channel catfish.…”

Section: Structural Components (Adhesins)mentioning

confidence: 99%

Aeromonas spp. whole genomes and virulence factors implicated in fish disease

Beaz-Hidalgo

Figueras

2013

Journal of Fish Diseases

249

187

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It is widely recognized that Aeromonas infections produce septicaemia, and ulcerative and haemorrhagic diseases in fish, causing significant mortality in both wild and farmed freshwater and marine fish species that damage the economics of the aquaculture sector. The descriptions of the complete genomes of Aeromonas species have allowed the identification of an important number of virulence genes that affect the pathogenic potential of these bacteria. This review will focus on the most relevant information derived from the available Aeromonas genomes in relation to virulence and on the diverse virulence factors that actively participate in host adherence, colonization and infection, including structural components, extracellular factors, secretion systems, iron acquisition and quorum sensing mechanisms.

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Section: Structural Components (Adhesins)mentioning

confidence: 99%

Aeromonas spp. whole genomes and virulence factors implicated in fish disease

Beaz-Hidalgo

Figueras

2013

Journal of Fish Diseases

249

187

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“…Region 5 of Vibrio and Pseudomonas includes a gene that encodes the motor protein MotY, however in Aeromonas it contains the master regulatory genes ( flrABC ). Recently, the Aeromonas MotY has been found in a different region with different behavior than the Vibrio MotY [179]. …”

Section: Motility and Flagellamentioning

confidence: 99%

The MainAeromonasPathogenic Factors

2012

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The members of the Aeromonas genus are ubiquitous, water-borne bacteria. They have been isolated from marine waters, rivers, lakes, swamps, sediments, chlorine water, water distribution systems, drinking water and residual waters; different types of food, such as meat, fish, seafood, vegetables, and processed foods. Aeromonas strains are predominantly pathogenic to poikilothermic animals, and the mesophilic strains are emerging as important pathogens in humans, causing a variety of extraintestinal and systemic infections as well as gastrointestinal infections. The most commonly described disease caused by Aeromonas is the gastroenteritis; however, no adequate animal model is available to reproduce this illness caused by Aeromonas. The main pathogenic factors associated with Aeromonas are: surface polysaccharides (capsule, lipopolysaccharide, and glucan), S-layers, iron-binding systems, exotoxins and extracellular enzymes, secretion systems, fimbriae and other nonfilamentous adhesins, motility and flagella.

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“…Proper stator recruitment and activity has been demonstrated to depend on several components within the flagellar motor. One of these components is the T-ring which is formed by the MotX and Y proteins in many Na 1 -dependent motors, such as those of Vibrio, Shewanella or Aeromonas (Terashima et al, 2006;Koerdt et al, 2009;Molero et al, 2011). MotX and MotY are also needed for MotAB function in S. oneidensis .…”

Section: Motxy Are Required For Motab* Activitymentioning

confidence: 99%

Mutations targeting the plug‐domain of the Shewanella oneidensis proton‐driven stator allow swimming at increased viscosity and under anaerobic conditions

Brenzinger

Dewenter

Delalez

et al. 2016

Molecular Microbiology

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Shewanella oneidensis MR-1 possesses two different stator units to drive flagellar rotation, the Na -dependent PomAB stator and the H -driven MotAB stator, the latter possibly acquired by lateral gene transfer. Although either stator can independently drive swimming through liquid, MotAB-driven motors cannot support efficient motility in structured environments or swimming under anaerobic conditions. Using ΔpomAB cells we isolated spontaneous mutants able to move through soft agar. We show that a mutation that alters the structure of the plug domain in MotB affects motor functions and allows cells to swim through media of increased viscosity and under anaerobic conditions. The number and exchange rates of the mutant stator around the rotor were not significantly different from wild-type stators, suggesting that the number of stators engaged is not the cause of increased swimming efficiency. The swimming speeds of planktonic mutant MotAB-driven cells was reduced, and overexpression of some of these stators caused reduced growth rates, implying that mutant stators not engaged with the rotor allow some proton leakage. The results suggest that the mutations in the MotB plug domain alter the proton interactions with the stator ion channel in a way that both increases torque output and allows swimming at decreased pmf values.

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Aeromonas hydrophila motY is essential for polar flagellum function, and requires coordinate expression of motX and Pom proteins

Cited by 17 publications

References 44 publications

Aeromonas spp. whole genomes and virulence factors implicated in fish disease

Aeromonas spp. whole genomes and virulence factors implicated in fish disease

The MainAeromonasPathogenic Factors

Mutations targeting the plug‐domain of the Shewanella oneidensis proton‐driven stator allow swimming at increased viscosity and under anaerobic conditions

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