Aeromonas hydrophila typing scheme based on patterns of agglutination with erythrocytes and yeast cells

Abstract

An agglutination typing scheme has been developed for strains of Aeromonas hydrophila. Primary agglutination typing is based on testing agar-grown A. hydrophila cells with human, horse, rat, and guinea pig erythrocytes and Saccharomyces cerevisiae cells. Further subdivision of primary groups is based firstly on whether yeast cell agglutination is inhibited by a D-mannose polymer, yeast mannan, and secondly on patterns of inhibition of hemagglutination by yeast mannan and the monomeric sugars L-fucose, D-galactose, and D-mannose. A total of 320 isolates were tested, and these were divisible into 39 distinct types on the basis of this scheme. Application of this typing scheme in the future to isolates of A. hydrophila known to be associated with human infection may enable correlations to be made between particular agglutination types and human pathogenicity.

Pili of Aeromonas hydrophila: Purification, Characterization, and Biological Role

“…Therefore, when an investigation on the colonization factors of a certain pathogen is planned, the investigators usually focus their attention on the pili and/or hemagglutinins in Aeromonas. Approximately 40 different types are known on the basis of erythrocytes species, co-agglutination with yeast, and the carboxyhydrate inhibition test (1). Although one of the hemagglutinins reported by Atkinson et al (A6-type hemagglutinin isolated from outer membrane proteins of Aeromonas) is probably important in adhesion to the intestinal epithelium (7), the role of other hemagglutinins and pili is still unclarified.…”

Mechanisms of diarrhoea

“…Aeromonas spp. can adhere to intestinal brush borders (Levett and Daniel, 1988), and agglutination of erythrocytes or yeast cells has been reported (Adams et al, 1983) although the pathogenic significance of this is uncertain.…”

Production and characterization of monoclonal antibodies to Aeromanas sobria surface antigens

“…Haemagglutination by bacterial cells was done on glass slides with pooled human O erythrocytes according to the method of Adams et al [19]. Inhibition of haemagglutination by Mab was determined by mixing the bacterial suspension with 20 /xl of ascitic fluid before the addition of red blood cells.…”

“…Aeromonas species exhibit haemagglutination of erythrocytes from different species including those of man [19]. A. sobria 3767 shows mannose-sensitive HA of human erythrocytes.…”

“…It is not known whether this inhibition was due to a direct blocking of the ligand by the Mab or to steric hindrance. It is believed that the haemagglutinins of Aeromonas species are lectin-like and react with the complementary sugars on the host cells [19]. Atkinson et al have identified one haemagglutinin 521 as a 43-kDa protein [23].…”