Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3−

Abstract

Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a causative agent of diarrhea in humans. We investigated the effects of ASH on anion transport in human colonic epithelial cells. ASH increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external HCO3-. Iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. The results suggest that ASH activates HCO3- secretion, whose level correlates with the severity of diarrhea.

Distribution and antibacterial drug resistance ofAeromonas spp. from fresh and brackish waters in Southern Turkey

“…Aeromonas hydrophila is known as an important fish pathogen and many studies have demonstrated that this pathogen produces a discrete number of virulence factors (Uzel and Ucar, 2000;Thayumanavan et al, 2003;Wu et al, 2006). The pathogen A. sobria is associated with acute gastroenteritis in both adults and children, and it exerts its effects by means virulence factors (Potomski et al, 1987;Millership et al, 1992;Takahashi et al, 2006). Aeromonas caviae is a ubiquitous aquatic organism, has long been considered to be of low pathogenicity and its virulence mechanisms are still not clearly understood (Monteil et al, 2004).…”

Campylobacter jejuni infection suppressed Cl− secretion induced by CFTR activation in T-84 cells

“…Measurement of short-circuit current to study ion transport has been previously reported [15]. T-84 cells were grown on the transwell for 7 days in order to obtain an electrical resistance greater than 800 U/cm 2 .…”

Aeromonas sobria Induces Proinflammatory Cytokines Production in Mouse Macrophages via Activating NLRP3 Inflammasome Signaling Pathways

“…Previous studies showed that A. hydrophila-triggered NLRP3 inflammasome activation in macrophages was mediated by its produced cytotoxins, including aerolysin, hemolysin, and multifunctional repeat-in-toxin. For A. sobria, it could also produce cytotoxins, and hemolysinmediated HCO − 3 secretion was correlated with the severity of inflammatory diarrhea (Takahashi et al, 2006). Whether hemolysin is associated with the NLRP3 inflammasome activation in A. sobria-stimulated PMjs needs to be further explored.…”