Aeromonas spp. in drinking water and food: Occurrence, virulence potential and antimicrobial resistance

Carusi, Juliana; Kabuki, Dirce Yorika; de Seixas Pereira, Pedro Marques; Cabral, Lucélia

doi:10.1016/j.foodres.2023.113710

Cited by 7 publications

(4 citation statements)

References 83 publications

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“…Despite the significant evidence of the pathogenic effects on aquatic organisms and humans, the role of Aeromonas as a true foodborne pathogen continues to be questioned. Overall, the occurrence of Aeromonas in food has been mostly related to contamination with contaminated water [ 37 ]. The common physical–chemical treatments used for the purification of drinking water in a food plant are normally capable of devitalizing Aeromonas ; however, ineffective water supply management could be responsible for its contamination.…”

Section: Discussionmentioning

confidence: 99%

Use of Matrix-Assisted and Laser Desorption/Ionization Time-of-Flight Technology in the Identification of Aeromonas Strains Isolated from Retail Sushi and Sashimi

Nalbone,

Forgia,

Pirrone

et al. 2024

Pathogens

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The genus Aeromonas includes well-known pathogenic species for fishes and humans that are widely distributed in the aquatic environment and foods. Nowadays, one of the main issues related to wild Aeromonas isolates is their identification at the species level, which is challenging using classical microbiological and biomolecular methods. This study aims to test MALDI-TOF MS technology in the identification of Aeromonas strains isolated from n. 60 retail sushi and sashimi boxes using an implemented version of the SARAMIS software V4.12. A total of 43 certified Aeromonas strains were used to implement the SARAMIS database by importing the spectra obtained from their identification. The original SARAMIS version (V4.12) failed to recognize 62.79% of the certified strains, while the herein-implemented version (V4.12plus) allowed the identification of all the certified strains at least to the genus level with a match of no less than 85%. Regarding the sushi and sashimi samples, Aeromonas spp. was detected in n. 18 (30%) boxes. A total of 127 colonies were identified at the species level, with A. salmonicida detected as the most prevalent species, followed by A. bestiarum and A. caviae. Based on the results of the present study, we could speculate that MALDI-TOF technology could be a useful tool both for the food industry to monitor product contamination and for clinical purposes to make diagnoses effectively and quickly.

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Section: Discussionmentioning

confidence: 99%

Use of Matrix-Assisted and Laser Desorption/Ionization Time-of-Flight Technology in the Identification of Aeromonas Strains Isolated from Retail Sushi and Sashimi

Nalbone,

Forgia,

Pirrone

et al. 2024

Pathogens

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“…Intrinsic resistance, mutations in specific genes, efflux pumps and horizontal gene transference are considered relevant mechanisms for the emergence of antibiotic resistance among Aeromonas spp. and were recently reviewed by Carusi and colleagues [8]. The Antibiotics 2024, 13, 166 2 of 12 increased use of antibiotics in human, animal and plant health and the ability of microorganisms to assemble biofilms [9] contribute to a major public health concern regarding antibiotic treatment failure.…”

Section: Introductionmentioning

confidence: 99%

Aeromonas spp. in Freshwater Bodies: Antimicrobial Resistance and Biofilm Assembly

Nascimento,

Rodrigues,

Matias

et al. 2024

Antibiotics

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Aeromonas spp. are environmental bacteria able to infect animals and humans. Here, we aim to evaluate the role of biofilms in Aeromonas persistence in freshwater. Aeromonas were isolated from water and biofilm samples and identified by Vitek-MS and 16S rRNA sequencing. Antibiotic susceptibility profiles were determined according to EUCAST, and a crystal violet assay was used to assess biofilm assembly. MTT and the enumeration of colony-forming units were used to evaluate biofilm and planktonic Aeromonas susceptibility to chlorination, respectively. Identification at the species level was challenging, suggesting the need to improve the used methodologies. Five different Aeromonas species (A. salmonicida, A. hydrophila, A. media, A. popoffii and A. veronii) were identified from water, and one species was identified from biofilms (A. veronii). A. veronnii and A. salmonicida presented resistance to different antibiotics, whith the highest resistance rate observed for A. salmonicida (multiple antibiotic resistance index of 0.25). Of the 21 isolates, 11 were biofilm producers, and 10 of them were strong biofilm producers (SBPs). The SBPs presented increased tolerance to chlorine disinfection when compared with their planktonic counterparts. In order to elucidate the mechanisms underlying biofilm tolerance to chlorine and support the importance of preventing biofilm assembly in water reservoirs, further research is required.

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“…Previously considered opportunistic pathogens, only posing a threat to immunocompromised individuals, these bacteria now cause disease in immunocompetent populations as well [7]. Transmission is common via ingestion of contaminated food and water [16] leading to infection of the gastrointestinal tract [17,18]. Contact with contaminated water, mud, or sewage effluent can lead to soft tissue infection, especially through open wounds [19,20,21], with severe cases causing necrotizing fasciitis [16,22].…”

Section: Introductionmentioning

confidence: 99%

“…Transmission is common via ingestion of contaminated food and water [16] leading to infection of the gastrointestinal tract [17,18]. Contact with contaminated water, mud, or sewage effluent can lead to soft tissue infection, especially through open wounds [19,20,21], with severe cases causing necrotizing fasciitis [16,22].…”

Section: Introductionmentioning

confidence: 99%

Polar flagellin glycan metaheterogeneity ofAeromonas hydrophilastrain ATCC 7966^T

Fulton,

Mendoza-Barberà,

Tomás

et al. 2024

Preprint

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Motile pathogens often rely upon flagellar motility as an essential virulence factor and in many species the structural flagellin protein is glycosylated. This post-translational modification has been shown to be necessary for proper folding of the flagellin structural proteins and proper function of the flagellar filament in a number of bacterial species. Aeromonas hydrophila is a ubiquitous aquatic pathogen with a constitutively expressed polar flagellum. Using a suite of mass spectrometry techniques, the flagellin FlaA and FlaB structural proteins of A. hydrophila strain ATCC 7966 were shown to be glycosylated with significant metaheterogeneity: heterologous glycans were observed with variable site occupancy. The penta- and hexa-saccharide glycan chains contained a previously unreported pseudaminic acid derivative with a mass of 422 Da as the linking sugar, followed in sequence by two hexoses, an N-acetylglucosamine derivative, a deoxy N-acetylglucosamine derivative, and sometimes an additional N-acetylglucosamine.

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Aeromonas spp. in drinking water and food: Occurrence, virulence potential and antimicrobial resistance

Cited by 7 publications

References 83 publications

Use of Matrix-Assisted and Laser Desorption/Ionization Time-of-Flight Technology in the Identification of Aeromonas Strains Isolated from Retail Sushi and Sashimi

Use of Matrix-Assisted and Laser Desorption/Ionization Time-of-Flight Technology in the Identification of Aeromonas Strains Isolated from Retail Sushi and Sashimi

Aeromonas spp. in Freshwater Bodies: Antimicrobial Resistance and Biofilm Assembly

Polar flagellin glycan metaheterogeneity ofAeromonas hydrophilastrain ATCC 7966^T

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Aeromonas spp. in drinking water and food: Occurrence, virulence potential and antimicrobial resistance

Cited by 7 publications

References 83 publications

Use of Matrix-Assisted and Laser Desorption/Ionization Time-of-Flight Technology in the Identification of Aeromonas Strains Isolated from Retail Sushi and Sashimi

Use of Matrix-Assisted and Laser Desorption/Ionization Time-of-Flight Technology in the Identification of Aeromonas Strains Isolated from Retail Sushi and Sashimi

Aeromonas spp. in Freshwater Bodies: Antimicrobial Resistance and Biofilm Assembly

Polar flagellin glycan metaheterogeneity ofAeromonas hydrophilastrain ATCC 7966T

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Polar flagellin glycan metaheterogeneity ofAeromonas hydrophilastrain ATCC 7966^T