Aeromonas veronii, a new ornithine decarboxylase-positive species that may cause diarrhea

Abstract

In 1983, the vernacular name Enteric Group 77 was coined for a group of strains that had been referred to our laboratory as "possible Vibrio cholerae except for gas production." By DNA-DNA hybridization (hydroxyapatite, 31P), 8 of 10 strains of Enteric Group 77 were very highly related to the labeled strain 1169-83 (74 to 100% at 60°C and 75 to 100% at 75°C; percent divergence, 0.0 to 2.5). Type strains of six other Aeromonas species were 45 to 66% related (60°C) to strain 1169-83, but type strains of 27 Vibrio species were only 2 to 6% related. The name Aeromonas veronii is proposed for the highly related group of nine strains formerly known es Enteric Group 77. The type strain is designated as ATCC 35604 (CDC 1169-83). Strains of A. veronu grew well at 36°C and had positive reactions at this temperature for indole, methyl red, Voges-Proskauer, citrate, lysine and ornithine decarboxylases, DNase, lipase, and motility; the strains had negative reactions for arginine decarboxylase, H2S, urea, and malonate. The following sugars were fermented: D-glucose (acid and gas), cellobiose (seven of nine strains), D-galactose, maltose, D-mannitol, D-mannose, a-methyl-D-glucoside (eight of nine strains), salicin, sucrose, and trehalose. The following sugars were not fermented: adonitol, L-arabinose, D-arabitol, dulcitol, erythritol, myo-inositol, lactose, raffinose, L-rhamnose, D-sorbitol, and D-xylose. The positive ornithine decarboxylase reaction differentiates A. veronii from other Aeromonas species. The antibiogram ofA. veronii is typical of other Aeromonas strains (resistance to ampicillin and carbenicillin and susceptibility to most other agents). A. veronii strains were isolated from three clinical sources: respiratory secretions of four victims of drowning or near drowning in fresh water (probably not clinically significant); infected wounds of two patients previously exposed to fresh water (unknown clinical significance); and stools from three patients with diarrhea (probably clinically significant). * Corresponding author. t Dedicated to M. Véron and M. Popoff for their pioneering studies of the genus Aeromonas.

Phenotypic characteristics and pathogenicity of Aeromonas genomospecies isolated from common carp (Cyprinus carpio L.)

“…veronii corresponded phenotypically to Aerom. veronii biotype sobria according to the profile proposed by Hickman-Brenner et al (1987). They were positive for arginine and negative for ornithine and aesculin.…”

Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from environmental and clinical samples in Spain

“…encheleia HG16 and Aerom. popoffii HG17 (Allen et al 1983;Farmer et al 1986;Hickman-Brenner et al 1987;Hickman-Brenner et al 1988;Schubert and Hegazi 1988;Kuijper et al 1989;Altwegg et al 1990;Carnahan et al 1991a;Carnahan et al 1991b;Carnahan et al 1991c;Martínez-Murcia et al 1992;Esteve et al 1995;Ali et al 1996;Huys et al 1997).…”

Proteases of theAeromonas hydrophilacomplex: identification, characterization and relation to virulence in channel catfish,Ictalurus punctatus(Rafinesque)

“…eaviae. Other species, including A. veronii (Hickman-Brenner, MacDonald, Steigenvalt, Fanning, Brenner &Farmer 1987), have been proposed. Although the motile aeromonads are common saprophytes in the aquatic environment (Leclerk & Buttiaux 1962), they are best known as fish pathogens (Plumb 1979), and have been described as causing disease in a variety of other animals, including reptiles (Marcus 1971), amphibians (Sanarelli 1891), birds (Shane & Gifford 1984) and humans (Blat, Shanthakumari & Rajan 1974).…”