In cryptography the block ciphers are the mostly used symmetric algorithms. In the existing system the standard S-Box of Advanced Encryption Standard(AES) is performed using the irreducible polynomial equation in table form known as look-up tables(LUTs). For more security purposes, second-order reversible cellular automata based S-box is created. The security aspects of the S-Box used in the AES algorithm are evaluated using cryptographic properties like Strict Avalanche Criteria, Non-Linearity, Entropy, and Common Immunity Bias. The design of S-Box using second-order reversible Cellular Automata is better concerning security and dynamic aspect as compared to the classical S-boxes used Advanced Encryption Standard.