Aestuarium zhoushanense is a later heterotypic synonym of Marivivens donghaensis, and transfer of Paradonghicola geojensis to the genus Marivivens as Marivivens geojensis comb. nov

Abstract

The 16S rRNA genes of Aestuarium zhoushanense G7T and Paradonghicola geojensis FJ12T shared 100 % sequence identity with Marivivens donghaensis AM-4T. Phylogeny of 16S rRNA gene sequences showed that the three type strains formed a monophyletic clade within the genus Marivivens . Whole genome sequence comparisons showed that three type strains shared 46.7–69.7 % digital DNA–DNA hybridization, 92.1–96.4 % average nucleotide identity and 96.2–98.1 % average amino acid identity. The high 16S rRNA gene similarity values show that three type strains should belong to the same genus. The pan-genome of the five strains contained 5754 genes including 1877 core genes. Based on the principle of priority, we propose that A. zhoushanense Yu et al. 2019 is a later heterotypic synonym of M. donghaensis Park et al. 2016, and P. geojensis should be reclassified as Marivivens geojensis comb. nov., respectively.

Phylotaxonomic assessment based on four core gene sets and proposal of a genus definition among the families Paracoccaceae and Roseobacteraceae

“…S1a). Their relationships had been clarified very recently [18]. Zongyanglinia huanghaiensis CY05 T (GCA_009753675.1) was closely related to Pelagicola marinus DSW4-44 T (GCA_005771405.1) (Fig.…”

“…The families Paracoccaceae and Roseobacteraceae comprise metabolically, phenotypically and genotypically diverse members that play critical roles in marine and other environments. Currently, hundreds of genera have been reported within these families, which makes it difficult to recognize their diversity and taxonomy comprehensively [11][12][13][14][15][16][17][18]. In this study, a phylotaxonomic assessment of the families Paracoccaceae and Roseobacteraceae was conducted based on four gene sets, bac120, rhodo268, rp1 and rp2, using different methods, and members within them were updated, respectively.…”

“…The family 'Rhodobacteraceae' was named after the first described genus, Rhodobacter, which comprises metabolically, phenotypically and genotypically diverse members, and its description relied heavily on 16S rRNA gene analysis before 2020 [10,11]. As a consequence, hundreds of genera and species have been reported within this family for their essential ecological role in marine environments, and misclassifications have been a reoccurring problem, underscoring the instability of classifications based on the 16S rRNA gene [11][12][13][14][15][16][17][18][19]. In 2022, the family 'Rhodobacteraceae' was considered to be illegitimate because it contravenes Rule 51 of the International Code of Nomenclature of Prokaryotes (ICNP), and it was replaced by two families: Roseobacteraceae and Paracoccaceae [14,20,21].…”