The correspondence between G a -actions on affine varieties and locally nilpotent derivations of the coordinate algebras is generalized in the projective case to the correspondence between stratified G a -actions on smooth projective varieties V and regular vector fields on V which are effectively locally nilpotent with stratification. These notions with stratifications are inspired by explicit computations of G a -actions on the projective space P n as well as the Hirzebruch surface F n and the associated regular vector fields. Using partly these observations, we investigate the existence of A 1 -cylinders in Fano threefolds with rank one.