Affinities between various Stylosanthes species as shown by rhizobial, soil pH and geographic relationships

Date, R. A.; Burt, R.L.; Williams, W. T.

doi:10.1016/0304-3746(79)90026-x

Cited by 11 publications

(2 citation statements)

References 12 publications

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“…Several techniques have been used to investigate diversity in the genus. Some of these have included the use of rhizobial affinities (Date et al . 1979), M–A characters (Burt et al .…”

Section: Introductionmentioning

confidence: 99%

Geographical patterns of genetic variation in two species ofStylosanthesSw. using amplified fragment length polymorphism

et al. 2001

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Understanding the extent and distribution of genetic diversity within a species is essential for the development of effective conservation strategies. The objective of this study was to assess genetic variation using amplified fragment length polymorphisms (AFLP) in two species of the tropical legume genus Stylosanthes Sw. Annual, S. humilis (2n = 20) and perennial, S. viscosa (2n = 20) are found throughout tropical America, and are sympatric for much of their range of distribution. One hundred and eleven accessions, covering a wide geographical range, were selected for AFLP analysis. Binary data matrices derived from DNA banding patterns were analysed using the software programs NTSYS-PC and ARLEQUIN. Several accessions were found to be misidentified. Of the S. humilis accessions, the overall average similarity value was (0.72) slightly higher than the value obtained for S. viscosa (0.67). Cluster analysis and principal coordinate analysis grouped accessions from both species by geographical origin, with a few exceptions. Analysis of molecular variance (AMOVA) in S. humilis revealed 59.4% of the variation among groups formed from the cluster analysis. This was highly significant (P < 0.001). For S. viscosa AMOVA also revealed more variation among than within groups (66.5%). This was also highly significant (P < 0.001). The majority of accessions of both species conserved ex situ are of Brazilian and Venezuelan origin. This study has identified areas in Central America and Mexico for which novel genetic variation may be found and where conservation activities should be focused.

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“…Several techniques have been used to investigate diversity in the genus. Some of these have included the use of rhizobial affinities (Date et al . 1979), M–A characters (Burt et al .…”

Section: Introductionmentioning

confidence: 99%

Geographical patterns of genetic variation in two species ofStylosanthesSw. using amplified fragment length polymorphism

et al. 2001

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“…The S. guianensis complex has been subjected to taxonomic studies based on morphological and agronomic traits (Burt et al. 1971), seed protein patterns (Robinson and Mergarrity 1975), isozymes (Stace 1982), rhizobial affinities (Date et al. 1979), crossability (Cameron 1974), and pollen stainability of hybrids (Hacker et al.…”

mentioning

confidence: 99%

AFLP analysis of genetic variability among Stylosanthes guianensis accessions resistant and susceptible to the stylo anthracnose

Jiang

Zhou

et al. 2005

Plant Breeding

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Stylosanthes guianensis, belonging to the genus Stylosanthes, is one of the most important tropical forage legumes and is native to South and Central America and Africa. Anthracnose, caused by the fungus Colletotrichum gloeosporioides (Penz.) Sacc., is a major constraint to the extensive use of Stylosanthes as tropical forage. Forty-two accessions of S. guianensis were assessed with amplified fragment length polymorphism (AFLP) for genetic diversity and for resistance to anthracnose. In AFLP analysis, four selective primer combinations screened from 96 primer combinations were used to analyse these accessions, and a total of 225 clear bands were used for genetic similarity (GS) analysis, showing a 95.5% level of polymorphism on average. GS from 31.0% to 95.0% among the accessions was calculated with NTSYS-pc software. The dendrogram was constructed with unweighted pair group method of averages (UPGMA) based on the AFLP data, and five clusters were defined at 48% GS. Two typical strains of C. gloeosporioides from Stylosanthes in China were used for anthracnose resistance screening. Most of the plant accessions showed variation in the reaction to two strains, and the correlation of resistance had a value of 0.904 (P < 0.01), suggesting common resistance to the two strains. The resistance accessions were randomly distributed in different groups of UPGMA clustering. These results demonstrate that AFLP analysis is an efficient method for evaluating the genetic diversity among S. guianensis accessions.

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Plant Factors Affecting Nodulation and Symbiotic Nitrogen Fixation in Legumes

Graham¹

1984

Biological Nitrogen Fixation

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Affinities between various Stylosanthes species as shown by rhizobial, soil pH and geographic relationships

Cited by 11 publications

References 12 publications

Geographical patterns of genetic variation in two species ofStylosanthesSw. using amplified fragment length polymorphism

Geographical patterns of genetic variation in two species ofStylosanthesSw. using amplified fragment length polymorphism

AFLP analysis of genetic variability among Stylosanthes guianensis accessions resistant and susceptible to the stylo anthracnose

Plant Factors Affecting Nodulation and Symbiotic Nitrogen Fixation in Legumes

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