Affinity-bound growth factor within sulfated interpenetrating network bioinks for bioprinting cartilaginous tissues

Wang, Bin; Díaz-Payno, Pedro J.; Browe, David C.; Freeman, Fiona E.; Nulty, Jessica; Burdis, Ross; Kelly, Daniel J.

doi:10.1016/j.actbio.2021.04.016

Cited by 73 publications

(61 citation statements)

References 55 publications

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“…Based on this, cell-laden bioprinting alginate hydrogels are designed and prepared in recent years. For example, Yu et al developed a scaffold-free strategy on fabrication of an engineered scaffold-free alginate stretchable tissue strand as a novel robotassisted bio-ink, which achieved the construction of an 8-cm long tissue strand with the rapid fusion property, thereby avoiding the requirement for solid supports or molds (Yu et al, 2016 Embedding of alginate sulfate had a limited effect on the viscosity and shear-thinning properties, which could enable the high-fidelity bioprinting and support the MSC viability (Figure 5) (Wang et al, 2021). The rigidity of the bioprinting constructs was greatly improved compared to that of individual alginate or GelMA bio-ink.…”

Section: Alginatementioning

confidence: 99%

Advances of Hydrogel-Based Bioprinting for Cartilage Tissue Engineering

Han

Chang

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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Bioprinting has gained immense attention and achieved the revolutionized progress for application in the multifunctional tissue regeneration. On account of the precise structural fabrication and mimicking complexity, hydrogel-based bio-inks are widely adopted for cartilage tissue engineering. Although more and more researchers have reported a number of literatures in this field, many challenges that should be addressed for the development of three-dimensional (3D) bioprinting constructs still exist. Herein, this review is mainly focused on the introduction of various natural polymers and synthetic polymers in hydrogel-based bioprinted scaffolds, which are systematically discussed via emphasizing on the fabrication condition, mechanical property, biocompatibility, biodegradability, and biological performance for cartilage tissue repair. Further, this review describes the opportunities and challenges of this 3D bioprinting technique to construct complex bio-inks with adjustable mechanical and biological integrity, and meanwhile, the current possible solutions are also conducted for providing some suggestive ideas on developing more advanced bioprinting products from the bench to the clinic.

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Section: Alginatementioning

confidence: 99%

Advances of Hydrogel-Based Bioprinting for Cartilage Tissue Engineering

Han

Chang

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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“…Various bioactive molecules have been incorporated into bioinks to enhance their chondrogenic properties. For example, growth factors from the transforming growth factor (TGF) and bone morphogenic protein (BMP) families, including TGF-β1, TGF-β3, BMP-4, and BMP-6 have been successfully incorporated into bioinks to enhance the chondrogenic properties of 3D bioprinted constructs [45][46][47]. Zhu et al report the development of a gelatin methacrylate (GelMA)/polyethyleneglycol diacrylate (PEGDA) 3D bioprinted construct containing TGF-β1 embedded nanospheres for cartilage tissue engineering applications [45].…”

Section: Bioactivitymentioning

confidence: 99%

“…Zhu et al report the development of a gelatin methacrylate (GelMA)/polyethyleneglycol diacrylate (PEGDA) 3D bioprinted construct containing TGF-β1 embedded nanospheres for cartilage tissue engineering applications [45]. Wang et al demonstrated that incorporating TGF-β3 into alginate-GelMA bioprinted constructs enhanced their chondrogenic properties [46]. Sun et al developed 3D bioprinted gradient-structured MSC-laden constructs capable of the controlled release of TGF-β3 and BMP-4 and demonstrated their potential to support cartilage repair in vivo in a rabbit model [47].…”

Section: Bioactivitymentioning

confidence: 99%

Translational Application of 3D Bioprinting for Cartilage Tissue Engineering

et al. 2021

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Cartilage is an avascular tissue with extremely limited self-regeneration capabilities. At present, there are no existing treatments that effectively stop the deterioration of cartilage or reverse its effects; current treatments merely relieve its symptoms and surgical intervention is required when the condition aggravates. Thus, cartilage damage remains an ongoing challenge in orthopaedics with an urgent need for improved treatment options. In recent years, major advances have been made in the development of three-dimensional (3D) bioprinted constructs for cartilage repair applications. 3D bioprinting is an evolutionary additive manufacturing technique that enables the precisely controlled deposition of a combination of biomaterials, cells, and bioactive molecules, collectively known as bioink, layer-by-layer to produce constructs that simulate the structure and function of native cartilage tissue. This review provides an insight into the current developments in 3D bioprinting for cartilage tissue engineering. The bioink and construct properties required for successful application in cartilage repair applications are highlighted. Furthermore, the potential for translation of 3D bioprinted constructs to the clinic is discussed. Overall, 3D bioprinting demonstrates great potential as a novel technique for the fabrication of tissue engineered constructs for cartilage regeneration, with distinct advantages over conventional techniques.

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“…Wang and colleagues formulated bioactive ink by interpenetrating the alginate-GelMA network incorporated with TGF-β3 [ 47 ]. They found that the release of TGF-β3 significantly promotes cartilage ECM deposition.…”

Section: Bioactive Inksmentioning

confidence: 99%

Bioactive Inks Development for Osteochondral Tissue Engineering: A Mini-Review

Bakhtiary

Liu

Ghorbani

2021

Gels

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Nowadays, a prevalent joint disease affecting both cartilage and subchondral bone is osteoarthritis. Osteochondral tissue, a complex tissue unit, exhibited limited self-renewal potential. Furthermore, its gradient properties, including mechanical property, bio-compositions, and cellular behaviors, present a challenge in repairing and regenerating damaged osteochondral tissues. Here, tissue engineering and translational medicine development using bioprinting technology provided a promising strategy for osteochondral tissue repair. In this regard, personalized stratified scaffolds, which play an influential role in osteochondral regeneration, can provide potential treatment options in early-stage osteoarthritis to delay or avoid the use of joint replacements. Accordingly, bioactive scaffolds with possible integration with surrounding tissue and controlling inflammatory responses have promising future tissue engineering perspectives. This minireview focuses on introducing biologically active inks for bioprinting the hierarchical scaffolds, containing growth factors and bioactive materials for 3D printing of regenerative osteochondral substitutes.

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Affinity-bound growth factor within sulfated interpenetrating network bioinks for bioprinting cartilaginous tissues

Cited by 73 publications

References 55 publications

Advances of Hydrogel-Based Bioprinting for Cartilage Tissue Engineering

Advances of Hydrogel-Based Bioprinting for Cartilage Tissue Engineering

Translational Application of 3D Bioprinting for Cartilage Tissue Engineering

Bioactive Inks Development for Osteochondral Tissue Engineering: A Mini-Review

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