Affinity chromatography: A review of trends and developments over the past 50 years

Rodriguez, Elliott; Poddar, Saumen; Iftekhar, Sazia; Suh, Kwang I.; Woolfork, Ashley G.; Ovbude, Susan T.; Pekarek, Allegra; Walters, M. T.; Lott, Shae; Hage, David S.

doi:10.1016/j.jchromb.2020.122332

Cited by 146 publications

(81 citation statements)

References 303 publications

(756 reference statements)

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“…Upon completion of the immobilization process, the resulting sorbent was washed with distilled water and treated with a solution of human serum albumin to activate unbound functional groups to exclude nonspecific sorption. The sorption properties of the sample obtained during the study were evaluated using affinity chromatography [19,20]. 10 ml of human blood plasma with a concentration of total cholesterol 355.7 ± 12.3 mg / dL (9.2 ± 0.32 mmol / L) and LDL -216.5 ± 7.3 mg / dL (5.6 ± 0.2 mmol / L) was passed through a glass column with a filter containing 1 ml of the tested sorbent at a rate of 0.3 ml / min.…”

Section: Methodsmentioning

confidence: 99%

In-Vitro Study of the Properties of Components for the Synthesis of Sorbent for Low-Density Lipoprotein Apheresis

Bazhenova¹,

Guryanova²,

Guryanov³

et al. 2021

Pharmacophore

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The research was carried out based on the Stavropol State Medical University. This work aimed to study the fundamental possibility of creating an immunosorbent for LDL apheresis based on nonporous highly dispersed silicon dioxide and monospecific polyclonal antibodies. In the course of the study, a matrix was selected for the synthesis of an immunosorbent based on aerosil modified with dextran. Affinity-purified rabbit polyclonal antibodies to human apolipoprotein B (ApoB) were immobilized onto the matrix. The obtained sample of the immunosorbent under in-vitro conditions showed good sorption properties to LDL, which makes it promising for further work on the creation of a sorbent for LDL-apheresis. It is concluded that the fundamental possibility of creating an immunosorbent based on non-porous highly dispersed silicon dioxide and monospecific polyclonal antibodies has been shown, which makes it possible to effectively bind and remove LDL from the blood plasma.

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Section: Methodsmentioning

confidence: 99%

In-Vitro Study of the Properties of Components for the Synthesis of Sorbent for Low-Density Lipoprotein Apheresis

Bazhenova¹,

Guryanova²,

Guryanov³

et al. 2021

Pharmacophore

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“…Salting in/out Exploiting protein solubility by increasing salt concentration in the solution [184] Dialysis Using size exclusion to separate proteins from small molecules and ions that pass through a semi-permeable membrane [185] Gel filtration chromatography Separation of proteins based on size [186] Ion-exchange chromatography Separation of proteins based on their net charge [187,188] Affinity chromatography Exploiting the affinity of proteins for given chemical groups to separate them [189] High-Pressure Liquid Chromatography (HPLC)…”

Section: Technique Principle Referencesmentioning

confidence: 99%

Trends and Applications of Omics Technologies to Functional Characterisation of Enzymes and Protein Metabolites Produced by Fungi

Ijoma

Heri

Matambo

et al. 2021

JoF

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Identifying and adopting industrial applications for proteins and enzymes derived from fungi strains have been at the focal point of several studies in recent times. To facilitate such studies, it is necessary that advancements and innovation in mycological and molecular characterisation are concomitant. This review aims to provide a detailed overview of the necessary steps employed in both qualitative and quantitative research using the omics technologies that are pertinent to fungi characterisation. This stems from the understanding that data provided from the functional characterisation of fungi and their metabolites is important towards the techno-economic feasibility of large-scale production of biological products. The review further describes how the functional gaps left by genomics, internal transcribe spacer (ITS) regions are addressed by transcriptomics and the various techniques and platforms utilised, including quantitive reverse transcription polymerase chain reaction (RT-qPCR), hybridisation techniques, and RNA-seq, and the insights such data provide on the effect of environmental changes on fungal enzyme production from an expressional standpoint. The review also offers information on the many available bioinformatics tools of analysis necessary for the analysis of the overwhelming data synonymous with the omics approach to fungal characterisation.

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“…The nature of the interactions between the ligand and the target molecule must be well known for ligand selection. For high-affinity yield, the interaction between the ligand and the target molecule must be specific and stable under different ambient conditions [1,10,11].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, there has been increasing interest in the synthesis of new sorbents for purification and enrichment of biomolecules such as glycoproteins, phosphorylated proteins/peptides, histidine‐tagged proteins, histidine‐rich proteins, and various antibodies by bioaffinity chromatography techniques [1]. Phosphorylation is a common posttranslational modification (PTM) involved in the regulation of central cellular processes [2].…”

Section: Introductionmentioning

confidence: 99%

Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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Isolation or enrichment of biological molecules from complex biological samples is mostly a prerequisite in proteomics, genomics, and glycomics. Different techniques have been used to advance the efficiency of the purification of biological molecules. Bioaffinity chromatography is one of the most powerful technique that plays an important role in the isolation of target biological molecules by the specific interactions with ligands that are immobilized on different support materials. This review examines the recent developments in bioaffinity chromatography particularly over the past 5 years in the literature. Also properties of supports, immobilization techniques, types of binding agents, and methods used in bioaffinity chromatography applications are summarized.

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Affinity chromatography: A review of trends and developments over the past 50 years

Cited by 146 publications

References 303 publications

In-Vitro Study of the Properties of Components for the Synthesis of Sorbent for Low-Density Lipoprotein Apheresis

In-Vitro Study of the Properties of Components for the Synthesis of Sorbent for Low-Density Lipoprotein Apheresis

Trends and Applications of Omics Technologies to Functional Characterisation of Enzymes and Protein Metabolites Produced by Fungi

Recent trends in sorbents for bioaffinity chromatography

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