Affinity Chromatography of Exopolygalacturonate Lyase fromErwinia carotovorasubsp.carotovora

Effects of Hexametaphosphate on Soybean Pectic Polysaccharide Extraction

“…carotOl'ora IFO 13921 by the method of Kegoya et al 17) The reaction mixture (0.5 ml total volume) containing a sample (0.05%),…”

High Performance Liquid Chromatographic Measurement of the Degradation Limits of Pectate by Exopectinases

“…carotovora IFO 13921 by the affinity chromatographic method of Kegoya et al 6 ) Exo-PG was prepared from roots of carrots (Daucus carota L.) by the method of Hatanaka et al') Endo-PG of Kluyveromyces fragilis was purified by the affinity chromatographic method of Inoue et aC) The assay conditions for the three enzymes were similar to those described previously6.7) The pectate concentration of the reaction mixtures was expressed as galacturonate, which was measured by the carbozole method 8 ) The amount of the degradation product, galacturonate or unsaturated diga1acturonate, in the 'reaction mixtures was measured by high performance liquid chromatography (HPLC). The percentage of degradation of pectate was calculated from the amount of 1417 galacturonate or unsaturated digalacturonate released; I mole of unsaturated digalacturonate is equivalent to 2 moles of galacturonidic bonds split:…”