Affinity engineering of maltoporin: Variants with enhanced affinity for particular ligands

A mathematical model of specific cell adhesion: Release of specifically adhering populations by soluble ligands

“…The model also predicts the effect of ligands with different binding constants for the receptor. These predictions are consistent with release experiments using various soluble competing ligands (Ferenci & Lee, 1982;Clune et al, 1984;Roos, 1990). It can be readily modified to account for the role of a soluble competing receptor.…”

“…JWROOSandMAHJORTSO compound is present that binds to the receptor or ligand (Ferenci & Lee, 1982;Clune et al, 1984;Hertz et al, 1985;Roos & Hjortso, 1989). In a biofilm of mixed specifically adhering cells, the relative population balances can change dramatically in response to these removal forces.…”

Genetic analysis of sequences in maltoporin that contribute to binding domains and pore structure

“…The insertion of a third tyrosine codon at position 119 to 120, where two Tyr codons already existed in wild-type protein, is in a region where substitutions were found to increase the affinity of starch binding (7,18). The reduction in starch binding that resulted from the insertion was due to a loss of affinity, since lack of binding was also observed at the permissive temperature at which phage-binding was near normal.…”

“…The genetic analysis of the maltodextrin-binding site using affinity-chromatographic approaches was hampered by the limitation that each lamB mutant had to be isolated through repeated chromatographic enrichment cycles of bacterial populations (up to 27 in the case of Clune et al [7]). Also, the binding phenotype of each lamB recombinant or clone had to be previously tested in individual column chromatographic experiments.…”

Rate Constants of Sugar Transport Through Two LamB Mutants ofEscherichia coli: Comparison with Wild-type Maltoporin and LamB ofSalmonella typhimurium

“…The E. coli strains BW1402 (Y118F) and BW1042 (R8H) (Ferenci & Lee, 1982;Clune et al, 1984) were grown overnight in 400 ml of M9 medium supplemented with 8 ml of amino acids at 37°C. The cells were harvested and passed three times through a French pressure cell at 6 MPa and unbroken cells were removed by centrifugation.…”