Affinity Imaging of Red Blood Cells Using an Atomic Force Microscope

Grandbois, Michel; Dettmann, Wolfgang; Benoit, Martin; Gaub, Hermann E.

doi:10.1177/002215540004800516

Cited by 191 publications

(129 citation statements)

References 35 publications

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“…This correlates with a broad distribution of unbinding pathways and rupture forces in MD simulations. The obtained values describe a notably weak interaction compared to other lectin-carbohydrate bonds like concanavalin A (17) or Helix pomatia Lectin (18). Compared to surface plasmon resonance measurements (SPR) of the interaction between HA and fetuin (19), a highly glycosylated blood plasma protein, we observed dissociation rates that are about 10 3 times higher.…”

Section: Discussionmentioning

confidence: 74%

Influenza virus binds its host cell using multiple dynamic interactions

Sieben

Kappel

Zhu

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

130

102

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Influenza virus belongs to a wide range of enveloped viruses. The major spike protein hemagglutinin binds sialic acid residues of glycoproteins and glycolipids with dissociation constants in the millimolar range [Sauter NK, et al. (1992) Biochemistry 31:9609-9621], indicating a multivalent binding mode. Here, we characterized the attachment of influenza virus to host cell receptors using three independent approaches. Optical tweezers and atomic force microscopy-based single-molecule force spectroscopy revealed very low interaction forces. Further, the observation of sequential unbinding events strongly suggests a multivalent binding mode between virus and cell membrane. Molecular dynamics simulations reveal a variety of unbinding pathways that indicate a highly dynamic interaction between HA and its receptor, allowing rationalization of influenza virus-cell binding quantitatively at the molecular level.multivalency | adhesion | avidity | tropism

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Section: Discussionmentioning

confidence: 74%

Influenza virus binds its host cell using multiple dynamic interactions

Sieben

Kappel

Zhu

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

130

102

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“…The obvious potential for mapping distributions of biomolecules on cell surfaces has been exploited to image distribution of mannan polymers on the yeast cells [1194], sugar chains on tissue sections of the rat vomeronasal epithelium [1195], receptor-associated protein binding proteins on 3T3 fibroblasts [1196], vitronectin receptors on a murine osteoblastic cell [1197], vascular endothelial growth factor (VEGF) receptor on bovine aortic endothelial cells [1198], tyrosine kinase A on PC 12 nerve cells [1199], calcitonin receptors on bone cells [1200]. AFM tips functionalized with Helix pomatia lectin that interacts specifically with a glycolipid on group A red blood cells allowed discrimination by adhesion maps between group A and group 0 red blood cells [1201] (see Fig. 49).…”

Section: Force Volume Modementioning

confidence: 99%

Force measurements with the atomic force microscope: Technique, interpretation and applications

Butt

Cappella

Kappl

2005

Surface Science Reports

3,245

2,949

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“…(A) (B) To investigate the mechanical, chemical, and physical properties of the surface at various locations, FV imagines consisting of array of 32 × 32 or 64 64 force curves were recorded in parallel with topographic images on different samples [155][156][157][158]. For each force curve, the maximum vertical displacement, Z, was controlled to obtain the same maximum vertical deflection, and thus the same maximum applied force for all force curves.…”

Section: B) Force Volume Mode (Fv) and Adhesion Force Mapsmentioning

confidence: 99%

On the Adhesion between Fine Particles and Nanocontacts: An Atomic Force Microscope Study

et al. 2006

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To optimize the handling of fine powders in industrial applications, understanding the interaction forces between single powder particles is fundamental. The forces between colloidal particles dominate the behavior of a great variety of materials, including paints, paper, soil, and many industrial processes. With the invention of the atomic force microscope (AFM), the direct measurement of the interaction between single micron-sized particles became possible. The adhesional contact between a particle and a substrate is a parameter for analyzing pull-off force data generated by AFM. The aim of this study was to understand surface interactions between fine particles. I measured the adhesion forces between AFM tips or particles attached to AFM cantilevers and different solid samples. Smooth and homogeneous surfaces such as silicon wafer, mica, or highly oriented pyrolytic graphite (HOPG), and more rough and heterogeneous surfaces such as iron particles or patterns of TiO 2 nanoparticles on silicon wafer were used. First, I addressed to the wellknown issue that AFM adhesion experiment results show wide distributions of adhesion forces rather than a single value. My experimental results show that variations in adhesion forces comprise fast (i.e., from one force curves to the next) random fluctuations and slower fluctuations, which occur over tens or hundreds of consecutive measurements. Slow fluctuations are not likely to be the result of variations in external factors such as lateral position, temperature, humidity, and so forth because those were kept constant. Even if two solid bodies are brought into contact under precisely the same conditions (same place, load, direction, etc.) the result of such a measurement will often not be the same as for the previous contact. The measurement itself will induce structural changes in the contact region which can change the value for the next adhesion force measurement.In the second part I studied the influence of humidity on the adhesion of nanocontacts. Humidity was adjusted relatively fast to minimize tip wear during one experiment. For hydrophobic surfaces, no signification change of adhesion force with humidity was observed. Adhesion force-versus-humidity curves recorded with hydrophilic surfaces either showed a maximum or continuously increased. I demonstrate that the results can be interpreted with simple continuum theory of the meniscus force. The meniscus force is calculated based on a model that includes surface roughness and takes into account different AFM tip (or particle) shapes by a two-sphere-model.Experimental and theoretical results show that the precise contact geometry has a critical influence on the humidity dependence of the adhesion force.Changes of tip geometry on the sub-10-nm length scale can completely change adhesion force-versus-humidity curves. Our model can also explain the differences between earlier AFM studies, where different dependencies of the adhesion force on humidity were observed.Keywords: Atomic force microscopy (AFM), cantile...

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Affinity Imaging of Red Blood Cells Using an Atomic Force Microscope

Cited by 191 publications

References 35 publications

Influenza virus binds its host cell using multiple dynamic interactions

Influenza virus binds its host cell using multiple dynamic interactions

Force measurements with the atomic force microscope: Technique, interpretation and applications

On the Adhesion between Fine Particles and Nanocontacts: An Atomic Force Microscope Study

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