Agribusiness plays a key role in the sustainable economic development of rural poor by fulfilling daily needs. In South Asia, all the countries have a similar pattern of societies, resources, climates, practices, and people located close to each other. Crop cultivation, dairy production, fishery, and forestry are the main agribusiness sectors for trading agricultural produce in markets. In contrast, factors (i.e., global warming due to climate change, natural calamity, environmental pollution, unsafe foodstuff, labor unavailability, marketing limitations, and financial crisis) are responsible for a serious fatal to agribusiness activities. Unless we uproot challenges, agribusiness cannot contribute effectively to the economy of developing nations in South Asia. Thus, future strategies may be standing on contemporary scientific research approaches on crop science, restoring resources, controlling food quality, introducing modern types of machinery, best marketing practices, and inclusive financing.