Aflatoxin B1 degradation by microorganisms isolated from Kombucha culture

Taheur, Fadia Ben; Mansour, Chalbia; Jeddou, Khawla Ben; Machreki, Yasmine; Kouidhi, Bochra; Abdulhakim, Jawaher A; Chaieb, Kamel

doi:10.1016/j.toxicon.2020.03.004

Cited by 42 publications

(16 citation statements)

References 55 publications

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“…This was confirmed by Ben Taheur et al . (2020) who illustrated the reduction through the binding of AFs to the bacterial cell wall, particularly the gluco-manann component which showed better capability to bind AFs whether bacteria were viable or killed (by acid or heat).…”

Section: Discussionmentioning

confidence: 99%

Innovative application of postbiotics, parabiotics and encapsulated Lactobacillus plantarum RM1 and Lactobacillus paracasei KC39 for detoxification of aflatoxin M1 in milk powder

Fahim

Badr

Shehata

et al. 2021

Journal of Dairy Research

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This study aimed to evaluate aflatoxin M1 (AFM1) level in milk powder and infant milk formulae, in addition to applying innovative methods for AFM1 & AFB1 detoxification. Fifty random samples of milk powder and infant formulae (25 of each) were collected from the Egyptian markets for assessing AFM1 level using ELISA technique. Bioactive components comprising cell free supernatants (postbiotic), acid-dead cells (parabiotic) and the encapsulated-cells of Lactobacillus plantarum RM1 and Lactobacillus paracasei KC39 were evaluated for their antifungal activity against toxigenic mold strains and their impact on AFB1 and AFM1 reduction in reconstituted milk powder. AFM1 concentration in unpacked milk powder was higher than that of packed samples and infant formulae, although these differences were not significant (P > 0.05). About 96.0, 29.4 and 25.0% of the tested infant formulae, unpacked, and packed milk powder were unacceptable in terms of the AFM1 limit defined by Egyptian and European standards, while all samples were in accordance with the USA/FDA standard. All tested mycotoxigenic strains were sensitive to the different treatments of the probiotics with the highest sensitivity regarding Fusarium strain with L. paracasei KC39 compared to other genera. The degradation ratios of AFM1 using the bioactives of the L. paracasei KC39 were higher than that of L. plantarum RM1 bioactives. Additionally, KC39 parabiotic manifested the best AFB1 reduction (60.56%). In conclusion, the positive and highly significant relationship (P < 0.05) between these effective biocompounds mirrors their major detoxification role which gives a safe solution for AFs contamination issues in milk and milk products.

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Section: Discussionmentioning

confidence: 99%

Innovative application of postbiotics, parabiotics and encapsulated Lactobacillus plantarum RM1 and Lactobacillus paracasei KC39 for detoxification of aflatoxin M1 in milk powder

Fahim

Badr

Shehata

et al. 2021

Journal of Dairy Research

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“…The cytotoxic effect of the E. alata extracts was evaluated on VERO cell line as described by Taheur et al (2020) . The concentrated extracts were diluted in RPMI 1640 medium to obtain concentration ranging from 100 to 1000 µg/mL.…”

Section: Methodsmentioning

confidence: 99%

Control of Staphylococcus aureus methicillin resistant isolated from auricular infections using aqueous and methanolic extracts of Ephedra alata

Dbeibia

Taheur

Altammar

et al. 2022

Saudi Journal of Biological Sciences

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“…By contrast, dominant yeast genera appears to be substantially more variable from study to study. Zygosaccharomyces [ 1 , 18 ], Candida [ 14 ], Torulaspora [ 1 ], Pichia [ 19 , 20 ], Brettanomyces [ 4 , 14 , 16 , 21 ], Schizosaccharomyces [ 1 , 19 , 21 ], Hanseniaspora [ 4 ], and Saccharomyces [ 1 , 2 , 18 , 22 ] are some of the genera described as important for Kombucha production. Yeast diversity in liquid and solid phases have observed the same taxa in both, but different patterns of succession during the course of fermentation [ 4 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Microbial Composition of SCOBY Starter Cultures Used by Commercial Kombucha Brewers in North America

Harrison

Curtin

2021

Microorganisms

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Kombucha fermentation is initiated by transferring a solid-phase cellulosic pellicle into sweetened tea and allowing the microbes that it contains to initiate the fermentation. This pellicle, commonly referred to as a symbiotic culture of bacteria and yeast (SCOBY), floats to the surface of the fermenting tea and represents an interphase environment, where embedded microbes gain access to oxygen as well as nutrients in the tea. To date, various yeast and bacteria have been reported to exist within the SCOBY, with little consensus as to which species are essential and which are incidental to Kombucha production. In this study, we used high-throughput sequencing approaches to evaluate spatial homogeneity within a single commercial SCOBY and taxonomic diversity across a large number (n = 103) of SCOBY used by Kombucha brewers, predominantly in North America. Our results show that the most prevalent and abundant SCOBY taxa were the yeast genus Brettanomyces and the bacterial genus Komagataeibacter, through careful sampling of upper and lower SCOBY layers. This sampling procedure is critical to avoid over-representation of lactic acid bacteria. K-means clustering was used on metabarcoding data of all 103 SCOBY, delineating four SCOBY archetypes based upon differences in their microbial community structures. Fungal genera Zygosaccharomyces, Lachancea and Starmerella were identified as the major compensatory taxa for SCOBY with lower relative abundance of Brettanomyces. Interestingly, while Lactobacillacae was the major compensatory taxa where Komagataeibacter abundance was lower, phylogenic heat-tree analysis infers a possible antagonistic relationship between Starmerella and the acetic acid bacterium. Our results provide the basis for further investigation of how SCOBY archetype affects Kombucha fermentation, and fundamental studies of microbial community assembly in an interphase environment.

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Aflatoxin B1 degradation by microorganisms isolated from Kombucha culture

Cited by 42 publications

References 55 publications

Innovative application of postbiotics, parabiotics and encapsulated Lactobacillus plantarum RM1 and Lactobacillus paracasei KC39 for detoxification of aflatoxin M1 in milk powder

Innovative application of postbiotics, parabiotics and encapsulated Lactobacillus plantarum RM1 and Lactobacillus paracasei KC39 for detoxification of aflatoxin M1 in milk powder

Control of Staphylococcus aureus methicillin resistant isolated from auricular infections using aqueous and methanolic extracts of Ephedra alata

Microbial Composition of SCOBY Starter Cultures Used by Commercial Kombucha Brewers in North America

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