Aflatoxin B1-induced ultrastructural alterations in matureZea mays embryos

McLean, Michelle

doi:10.1007/bf01138481

Cited by 4 publications

(4 citation statements)

References 36 publications

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“…The major bands in these Raman shift regions may be associated with fungal cellular metabolites and modified cell membrane and inhibited protein and starch synthesis due to the effects of fungal and aflatoxin infection of kernels. 25 The observed Raman bands in the SERS spectra were well assigned to the main vibrational modes associated with aflatoxin molecules according to the previously reported results. 18,26 However, shifts of some aflatoxin bands, in particular, in the aflatoxin-related Raman spectral regions, were easily observed compared to corresponding bands of conventional Raman spectroscopy.…”

Section: ■ Materials and Methodssupporting

confidence: 81%

“…Qualitative spectral differences in the normalized and derivative spectra were observed in the Raman shift ranges of 400–530 cm –1 , 800–980 cm –1 , 1100–1250 cm –1 , and 1500–1800 cm –1 . The major bands in these Raman shift regions may be associated with fungal cellular metabolites and modified cell membrane and inhibited protein and starch synthesis due to the effects of fungal and aflatoxin infection of kernels …”

Section: Resultsmentioning

confidence: 99%

“…The PCA and PLS results also provide some additional information on the particular spectral subranges and bands corresponding to the level of aflatoxins in maize sample extracts (data not shown). Some spectral regions and wavelengths with higher loading values in PCA and with higher absolute regression coefficient value in PLS are associated with an increased level of aflatoxins and changes in maize kernels induced by aflatoxins . Cluster analysis performed using all the preprocessed spectra allowed classification of aflatoxin contamination samples, which was basically configured by the aflatoxin group.…”

Section: Resultsmentioning

confidence: 99%

“…Some spectral regions and wavelengths with higher loading values in PCA and with higher absolute regression coefficient value in PLS are associated with an increased level of aflatoxins and changes in maize kernels induced by aflatoxins. 25 Cluster analysis performed using all the preprocessed spectra allowed classification of aflatoxin contamination samples, which was basically configured by the aflatoxin group. However, the classification results obtained from the best clustering algorithm employed (hierarchical Ward's minimum variance method) were not as good as those from the canonical functions.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Feasibility of Surface-Enhanced Raman Spectroscopy for Rapid Detection of Aflatoxins in Maize

Lee

Herrman

Bisrat

et al. 2014

J. Agric. Food Chem.

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Rapid and sensitive surface-enhanced Raman spectroscopy (SERS) for aflatoxin detection was employed for development of the models to classify and quantify aflatoxin levels in maize at concentrations of 0 to 1,206 μg/kg. Highly effective SERS substrate (Ag nanosphere) was prepared and mixed with a sample extract for SERS measurement. Strong Raman bands associated with aflatoxins and changes in maize kernels induced by aflatoxin contamination were observed in different SERS spectroscopic regions. The k-nearest neighbors (KNN) classification model yielded high classification accuracy and lower prediction error with no misclassification of contaminated samples as aflatoxin negative. The multiple linear regression (MLR) models showed a higher predictive accuracy with stronger correlation coefficients (r = 0.939-0.967) and a higher sensitivity with lower limits of detection (13-36 μg/kg) and quantitation (44-121 μg/kg) over other quantification models. Paired sample t test exhibited no statistically significant difference between the reference values and the predicted values of SERS in most chemometric models. The proposed SERS method would be a more effective and efficient analytical tool with a higher accuracy and lower constraints for aflatoxin analysis in maize compared to other existing spectroscopic methods and conventional Raman spectroscopy.

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Section: ■ Materials and Methodssupporting

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Feasibility of Surface-Enhanced Raman Spectroscopy for Rapid Detection of Aflatoxins in Maize

Lee

Herrman

Bisrat

et al. 2014

J. Agric. Food Chem.

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Application of near infrared spectroscopy for rapid detection of aflatoxin B1 in maize and barley as analytical quality assessment

et al. 2009

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Biorepress of fumonisin B1 production and their phytotoxicity on growth and ultrastructures of Maize (Zea mays) Seedlings

Abboud

2012

Egyptian Academic Journal of Biological Sciences, G. Microbiolo

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The results clearly indicated that the seed germinibility in the presence of FB1 was decreased with increasing their concentration, whereas the germinibility was uncompletely ceased at high concentrations, a slight initial lag in germination observed at lowest concentration 28.57 µg /ml and highest concentration 64.11 µg /ml, respectively compared with the control. At concentration of FB1 (28.57 µg /ml), elongation of the hypocotyls and epicotyls in seedlings slightly inhibited compared with the control. Chlorophyll a and b content of seedlings of Zea mays decreased with increasing concentrations of Fumonisin B1. Chlorophyll a content was 6.23, 3.88 and 3.91 (mg/ g fresh weight) at concentrations 28.57, 40.54 and 64.11 (µg /ml) of Fumonisin B1 compared with the control 8.55 mg/ g fresh weight. The ultrastructural studies indicate that there were dramatic differences between the treated and untreated tissues of Zea mays seedlings with the toxin FB1. The treated cells with FB1 are highly vacuolated compared with the untreated cells. Thin layer of external epidermis was observed in untreated cells while thick layer of external epidermis in treated cells was appeared. Sacchaaromyces cerevisiae reduced FB1 to 40.54 (µg /ml) compared with control 64.11(µg /ml) .On the other hand Trichoderma harizianum was more efficient in FB1 productivity where productivity was reduced to 28.57 (µg /ml) with 44.56 %.

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Aflatoxin B1-induced ultrastructural alterations in matureZea mays embryos

Cited by 4 publications

References 36 publications

Feasibility of Surface-Enhanced Raman Spectroscopy for Rapid Detection of Aflatoxins in Maize

Feasibility of Surface-Enhanced Raman Spectroscopy for Rapid Detection of Aflatoxins in Maize

Application of near infrared spectroscopy for rapid detection of aflatoxin B1 in maize and barley as analytical quality assessment

Biorepress of fumonisin B1 production and their phytotoxicity on growth and ultrastructures of Maize (Zea mays) Seedlings

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