Aflatoxin B1 induces Src phosphorylation and stimulates lung cancer cell migration

Cui, Anguo; Hua, Hui; Shao, Ting; Song, Peiying; Kong, Qingbin; Luo, Ting; Jiang, Yangfu

doi:10.1007/s13277-015-3341-2

Cited by 26 publications

(10 citation statements)

References 32 publications

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“…Longer-term studies of the effects of aflatoxins on mucociliary transport, potentially in an animal model of aflatoxin-exposure, will help to shed light on situations of chronic exposure and effects on airways. To our knowledge, studies of longer term exposures have only been previously done in airway cells 34 51 52 53 54 55 56 57 and animals 28 58 59 60 61 62 with a focus on carcinogenesis. The concentrations of aflatoxins used here (0.1–10 μM) are in the same range used in previous in vitro and ex vivo studies by other groups 4 52 53 56 57 .…”

Section: Discussionmentioning

confidence: 99%

Fungal Aflatoxins Reduce Respiratory Mucosal Ciliary Function

Lee

Workman

Carey

et al. 2016

Sci Rep

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Aflatoxins are mycotoxins secreted by Aspergillus flavus, which can colonize the respiratory tract and cause fungal rhinosinusitis or bronchopulmonary aspergillosis. A. flavus is the second leading cause of invasive aspergillosis worldwide. Because many respiratory pathogens secrete toxins to impair mucociliary immunity, we examined the effects of acute exposure to aflatoxins on airway cell physiology. Using air-liquid interface cultures of primary human sinonasal and bronchial cells, we imaged ciliary beat frequency (CBF), intracellular calcium, and nitric oxide (NO). Exposure to aflatoxins (0.1 to 10 μM; 5 to 10 minutes) reduced baseline (~6–12%) and agonist-stimulated CBF. Conditioned media (CM) from A. fumigatus, A. niger, and A. flavus cultures also reduced CBF by ~10% after 60 min exposure, but effects were blocked by an anti-aflatoxin antibody only with A. flavus CM. CBF reduction required protein kinase C but was not associated with changes in calcium or NO. However, AFB2 reduced NO production by ~50% during stimulation of the ciliary-localized T2R38 receptor. Using a fluorescent reporter construct expressed in A549 cells, we directly observed activation of PKC activity by AFB2. Aflatoxins secreted by respiratory A. flavus may impair motile and chemosensory functions of airway cilia, contributing to pathogenesis of fungal airway diseases.

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Section: Discussionmentioning

confidence: 99%

Fungal Aflatoxins Reduce Respiratory Mucosal Ciliary Function

Lee

Workman

Carey

et al. 2016

Sci Rep

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“…In detail, Western blot analysis was to evaluate IRS expression and Src, Akt and ERK phosphorylation in lung cancer cell lines, A549 and SPCA-1, after AFB1 treatment. These studies showed that AFB1: (i) up-regulates IRS1 and IRS2; (ii) induces Src, Akt and ERK1/2 phosphorylation; (iii) stimulates lung cancer cell migration, which can be inhibited by saracatinib [ 71 ].…”

Section: Aflatoxins Related Diseasesmentioning

confidence: 99%

Aflatoxin B1 and M1: Biological Properties and Their Involvement in Cancer Development

Marchese

Polo

Ariano

et al. 2018

Toxins

424

255

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Aflatoxins are fungal metabolites found in feeds and foods. When the ruminants eat feedstuffs containing Aflatoxin B1 (AFB1), this toxin is metabolized and Aflatoxin M1 (AFM1) is excreted in milk. International Agency for Research on Cancer (IARC) classified AFB1 and AFM1 as human carcinogens belonging to Group 1 and Group 2B, respectively, with the formation of DNA adducts. In the last years, some epidemiological studies were conducted on cancer patients aimed to evaluate the effects of AFB1 and AFM1 exposure on cancer cells in order to verify the correlation between toxin exposure and cancer cell proliferation and invasion. In this review, we summarize the activation pathways of AFB1 and AFM1 and the data already reported in literature about their correlation with cancer development and progression. Moreover, considering that few data are still reported about what genes/proteins/miRNAs can be used as damage markers due to AFB1 and AFM1 exposure, we performed a bioinformatic analysis based on interaction network and miRNA predictions to identify a panel of genes/proteins/miRNAs that can be used as targets in further studies for evaluating the effects of the damages induced by AFB1 and AFM1 and their capacity to induce cancer initiation.

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“…Liu and Wu [30] reported in their study that people with chronic hepatitis B infection and exposed to aflatoxin B1 have a high risk of developing liver cancer tenfold or more. Other studies have also highlighted the role of AFB1 in the development of other types of cancer such as kidney, respiratory and gastrointestinal cancer [31][32]. In addition to its carcinogenicity, AFB1 is an immunosuppressive compound, decreasing resistance to parasitic, bacterial and fungal infections.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Risk of Exposure to Aflatoxins Found in Maize (Zea mays L.) Produced in Cote d'ivoire in Ivorian Adults

Bamba

Coulibaly

Daouda

et al. 2021

AFSJ

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Aims: The objective of this study is to estimate the health risk incurred by the Ivorian adult consumer as a result of exposure to AFB1 and the sum of aflatoxins (AFT) through the consumption of maize. Place and Duration of Study: During this study 375 samples of maize (grains, cobs, spathes) were taken from February 2016 to January 2017 and the analyzes were carried out at the Biotechnology Laboratory, Agriculture and Development of Biological Resources of the Félix HOUPHOUËT-BOIGNY University. Methodology: The detection of aflatoxins (AFB1, AFB2, AFG1, and AFG2) was performed by HPLC using the AOAC method. The risk analysis procedure was conducted according to the Codex Alimentarius risk analysis model. It consisted of a quantitative assessment. The quantitative estimation of the aflatoxin exposure was performed according to the deterministic approach. Results: The results indicate the presence of aflatoxins B1, B2, G1 and G2 in all forms of maize (grains, cobs, spathes) and in the five regions visited. The average concentrations of aflatoxin B1 (AFB1) and the sum of aflatoxins (AFT) ranged from 0.79 ± 0.04 µg/kg to 130.31 ± 22.56 µg/kg and from 2.63 ± 2.35 µg/kg to 169.13 ± 40.39 µg/kg, respectively. Subsequently, the Estimated Daily Intakes (EDI) by consumption of aflatoxin-contaminated maize vary according to the level of contamination. They range from 0.28±0.00 ng/kg body weight/day to 52.85±9.14 ng/kg body weight/day for AFB1 exposure. They ranged from 1.00±0.85 ng/kg body weight/day to 68.57± 16.28 ng/kg body weight/day for AFT exposure. Samples of maize grains and cobs from the Gbèkê, Poro and Hambol regions showed low daily doses below the reference values (2.028 ng/kg body weight/day and 4.056 ng/kg body weight/day). The opposite results are observed with samples (grains, ears and spathes) from Indenié-Djuablin and Gountougo. Conclusion: The results of the daily doses of aflatoxin exposure of these regions show a need for monitoring their production. Thus, the implementation of efficient technical itineraries throughout the maize distribution chain, both during production, post-harvest processing, and conservation will be able to guarantee a better sanitary quality of this agricultural food product.

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Aflatoxin B1 induces Src phosphorylation and stimulates lung cancer cell migration

Cited by 26 publications

References 32 publications

Fungal Aflatoxins Reduce Respiratory Mucosal Ciliary Function

Fungal Aflatoxins Reduce Respiratory Mucosal Ciliary Function

Aflatoxin B1 and M1: Biological Properties and Their Involvement in Cancer Development

Assessment of the Risk of Exposure to Aflatoxins Found in Maize (Zea mays L.) Produced in Cote d'ivoire in Ivorian Adults

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