Aflatoxin carryover and clearance from tissues of laying hens

Wolzak, A.; Pearson, A. M.; Coleman, T. H.; Pestka, James J.; Gray, J. I.; Chen, C.

doi:10.1016/0278-6915(86)90262-0

Cited by 34 publications

(20 citation statements)

References 16 publications

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“…The ratio of the feed level to the residual level of AFB1 was 4615 in the egg yolk and 3846 in the albumen in chicken hens, and those values were higher than the respective values in the other birds. The ratio of the feed level to the residual level in laying hens and broiler chickens reported by others varies widely [4,9,10,13,16], but the observed values for chickens in this study are within the range of the reported values. Also consistent with others' observations, there was a difference in the tissue level of aflatoxin between layers and broilers, the levels being higher in laying hens than in broiler chickens.…”

supporting

confidence: 69%

Residues of Aflatoxins in the Liver, Muscle and Eggs of Domestic Fowls

Bintvihok

Thiengnin

Doi

et al. 2002

The Journal of Veterinary Medical Science

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ABSTRACT. The residue of aflatoxins in the liver, muscle and eggs of laying ducks, hens and quails and in broiler chickens was examined by conducting 7-day feeding experiments with a diet containing 3 ppm Aflatoxin B1 (AFB1). Birds were sacrificed on the 8th or 11th day of AFB1 feeding. AFB1 and its metabolites in the tissues and eggs were determined by HPLC. The tissue levels of AFB1 and its metabolites were higher in quail than in the other birds. The levels of AFB1 and its metabolites, including acid-hydrolyzable metabolites, were more than 10-fold higher in the liver than in the muscle in all the species. The ratio of AFB1 in the feed to the residual level in the liver was 383 in quail, but was ≥ 5769 in the other birds. The corresponding ratios in the egg yolk and albumen of AFB1 were 4615 and 3846, respectively, in chicken hens, and these values were higher than those in the other birds. KEY WORDS: aflatoxin B1, domestic fowl, residue.J. Vet. Med. Sci. 64(11): 1037-1039, 2002 Aflatoxins are toxic, carcinogenic secondary metabolites of Aspergillus flavus and A. parasiticus, aflatoxin B1 (AFB1) being the most toxic and frequently produced metabolite. There have been many observations of aflatoxin residues in the tissues and eggs of broiler chickens and laying hens [1-3, 7, 10, 11, 13], but little is known about aflatoxin residues in ducks and quails. In this study, we investigated the aflatoxin residue in the liver, muscle and eggs of laying ducks, hens and quails and in broiler chickens by conducting short-term feeding experiments, with an emphasis on comparison between species.The cultures of Aspergillus flavus (a local strain of the Department of Agriculture, Ministry of Agriculture and Cooperatives, Thailand) on potato dextrose agar were dissolved in distilled water, mixed with 30% moisture-commercial corn and kept in gunny sacks for 1-2 weeks to allow aflatoxin production. After being dried and ground, the contaminated corn was mixed with feed to give 3 ppm aflatoxin B1 concentration, and then kept in a refrigerator until used.Eighteen each 6-month-old Kaki Campbell laying ducks, 6-month-old laying hens that were cross-breed of Rhode Island Red (cock) and Bar Premutroc (hen) chickens and 40-day-old Arbor Acor broiler chickens (Bang-Pa-Kong 1 Breed from the Department of Livestock Development, Thailand), and sixty 2-month-old laying Japanese quails (Coturnix Coturnix Japonica) were purchased from farmers around Bangkok. For control groups, 6 laying ducks, laying hens and broiler chickens, and 20 laying quails, were given conventional feed free of aflatoxins. The other birds were fed a diet containing 3 ppm AFB1 for 7 days, from the morning of the first day until the evenng of the 8th day. In preliminary experiments, no birds died and AFB1 was detected in the livers of the birds with this feeding regime.Half of the control and aflatoxin-fed groups were sacrificed on the 8th day, and the remaining birds were sacrified on the 11th day, 3 days after withdrawal of the contaminated feed.The liver and muscle we...

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supporting

confidence: 69%

Residues of Aflatoxins in the Liver, Muscle and Eggs of Domestic Fowls

Bintvihok

Thiengnin

Doi

et al. 2002

The Journal of Veterinary Medical Science

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“…The aflatoxin analysis of animal tissues has been done with thin-layer chromatography (TLC) using visual estimation (Jacobson and Wiseman 1974) or with densitometric quantification (Wolzak et al 1985(Wolzak et al , 1986, by high-performance liquid chromatography (HPLC) (Micco et a1 1988, Beaver et a1 1990 and in recent years by enzyme-linked immunosorbent assay (ELISA) (Adachi et a1 1991) and by a fluorotoxinometer method, the latter being used for detection of aflatoxins in tissues from naturally intoxicated birds (Kaur et a1 1991). The 407 lowest detection limits have been achieved with HPLC and TLC using fluorodensitometry.…”

Section: Introductionmentioning

confidence: 99%

Aflatoxin and its metabolites in tissues from laying hens and broiler chickens fed a contaminated diet

et al. 1994

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Two groups of 32 hens and broiler chickens were contaminated with 2.5 and 5 mg of aflatoxin (AF) kg-' feed for a period of 32 days. During this contamination 16 birds were sacrificed and aflatoxin and its metabolites were detected using thin-layer chromatography and fluorescence densitometry. The tissues analysed (liver, muscle, kidney, gizzard and eggs) gave a wide range of concentrations, the lowest was found in hen muscle (0.05 pg kg-' of AFB,) and the highest in gizzards from the 5 mg kg-' group of the hens (9.01 pg kg-' of AFB,). Metabolites of AFB,, AFM, and AFB,, appeared in the liver but not in other tissues. In broiler's tissues, the following metabolities were isolated : AFM and AFB,, in liver, aflatoxicol in muscle and AFM, and AFB,, in kidneys, all having concentrations lower than AFB,. Aflatoxicol was isolated from one egg sample (0.32 pg kg-I). For both types of birds, aflatoxin clearance time was only 24 h for muscle and kidneys. In livers from the 5 mg kg-' group, AFM, and AFB,, were still found 4 days after removal of the contaminated feed. In eggs and gizzards, aflatoxin residue was still detected on the 8th day of the clearance period although in low quantities. In the broiler's gizzards, clearance time was only 24 h. These results suggest that aflatoxin transfer to edible tissues is very small and the danger of contaminations to humans is also very small, except in the case of gizzards.

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“…These generally include radioactivity, thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC) combined with different detectors 8,[21][22][23][24][25][26][27][28] . Radioactivity is very sensitive, which can be utilized in mass balance and tissue distribution studies, but not suitable for kinetic analysis due to the serious interferences of the extensive metabolites 29 .…”

Section: Introductionmentioning

confidence: 99%

Establishment of an isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rats

et al. 2012

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(2012). Establishment of an isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rat. Analytical Methods, 4 (11), 3708-3717. 10.1039/c2ay25891a Establishment of an isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rat AbstractAn isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with a fast sample preparation using homemade clean-up cartridges was developed for simultaneous determination of co-occurring mycotoxins exemplified with aflatoxin B1(AFB1) and T-2 toxin (T-2) in representative biomatrices of rat plasma, heart, liver, kidney, spleen, lung and brain in a total run time of 7 min. The established approach using stable

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Aflatoxin carryover and clearance from tissues of laying hens

Cited by 34 publications

References 16 publications

Residues of Aflatoxins in the Liver, Muscle and Eggs of Domestic Fowls

Residues of Aflatoxins in the Liver, Muscle and Eggs of Domestic Fowls

Aflatoxin and its metabolites in tissues from laying hens and broiler chickens fed a contaminated diet

Establishment of an isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rats

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