Aflatoxin Control in Maize by Trametes versicolor

Abstract

Aspergillus flavus is a well-known ubiquitous fungus able to contaminate both in pre- and postharvest period different feed and food commodities. During their growth, these fungi can synthesise aflatoxins, secondary metabolites highly hazardous for animal and human health. The requirement of products with low impact on the environment and on human health, able to control aflatoxin production, has increased. In this work the effect of the basidiomycete Trametes versicolor on the aflatoxin production by A. flavus both in vitro and in maize, was investigated. The goal was to propose an environmental loyal tool for a significant control of aflatoxin production, in order to obtain feedstuffs and feed with a high standard of quality and safety to enhance the wellbeing of dairy cows. The presence of T. versicolor, grown on sugar beet pulp, inhibited the production of aflatoxin B1 in maize by A. flavus. Furthermore, treatment of contaminated maize with culture filtrates of T. versicolor containing ligninolytic enzymes, showed a significant reduction of the content of aflatoxin B1.

Aflatoxin B1 and M1 Degradation by Lac2 from Pleurotus pulmonarius and Redox Mediators

“…Although aflatoxin degradation by fungal laccase enzymes has already been reported [ 15 , 25 , 26 ], to date no direct and unambiguous correlation between laccase and aflatoxin degradation has been described, since cultured filtrates or LC commercial preparation were tested in the degradation assays.…”

Molecular docking studies and in vitro degradation of four aflatoxins (AFB1, AFB2, AFG1, and AFG2) by a recombinant laccase from Saccharomyces cerevisiae

“…The use of laccases may result in rapid and significant recalcitrant substrate degradation (Ruiz‐Duenas & Martinez, 2009). Laccases have potential in the degradation of the aflatoxin which, was suggested by the different literatures (Alberts, Gelderblom, Botha, & van Zyl, 2009; Dellafiora, Galaverna, Reverberi, & Dall'Asta, 2017; Loi et al., 2016; Scarpari et al., 2014)…”

Current methods for mycotoxins analysis and innovative strategies for their reduction in cereals: an overview

“…Some notable exceptions were found during studies of enzymatic detoxification: peroxidases and laccases can also modify the substrate and may destroy valuable food components. It is reported that the laccase from Trametes versicolor is able to degrade AFB 1 . The potential for mycotoxin detoxification by enzymatic activities has been recently reviewed …”