Aflatoxin M1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer

Abstract

Aflatoxins are a kind of mycotoxins that are mostly produced by a group of molds such as Aspergillus flavus and Aspergillus parasiticus. The studies on different parts of Iran showed that AFM 1 infection of milk is higher than European Union's standard. In this study, the specific aptamer with the carboxylic group at the end of 5′ and Fe 3 O 4 nanoparticles with amine groups was synthesized. Morphological and structural qualities of Fe 3 O 4 were determined by the Fourier-transform infrared spectrograph (FTIR), dynamic light scattering, scanning electron microscope, and X-ray diffraction devices. The specificity of the aptamer to AFM 1 was investigated in adjacent of AFM 1 and aflatoxin B 1 . Then, aptamers were attached to nanoparticles to improve synthetic qualities and to ease of its detachment. The attachment was approved by FTIR method. The complex (Fe 3 O 4 -APT) was then added to infected milk and after the proper time was detached from the milk using a magnet. The remained amount of AFM 1 was attained in milk using high-performance liquid chromatography. Our result showed that this method for aflatoxin detoxification is much more effective than conventional methods based on recognition of AFM 1 and their concentration in infected milk. This method is more applicable, faster, and cheaper highlighting its application in milk factories. Graphical abstractKeywords Aflatoxin · Aptamer · Fe 3 O 4 nanoparticle · Detoxification · Mycotoxin

Aflatoxin detoxification by biosynthesized iron oxide nanoparticles using green and black tea extracts

“…Therefore, FeONPs were used in a new method with high specificity and sensitivity for aflatoxin M1 detoxification of milk in pasteurized milkproducing factories. The researchers claimed that their method was more feasible, faster, and cheaper than the current applications in dairy factories (Jouni et al 2018). Abdelghany et al (2020) investigated the synergistic effect of 50 ppm AgNPs and Juniperus procera stem extracts.…”