Aflatoxin M1 in Pasteurized Market Milk in Korea

Abstract

Aflatoxin M1, ingested as aflatoxin B1 via contaminated feedstuff and later converted into, is a major problematic target for milk safety control among the aflatoxin class. Korean government has controlled level of AFM1 in milk at 500 ppt as maximum residue level (MRL), and more recently, government also publicized the proposal for more strict control on fungal toxins about infant and baby foods. In this study the levels of Aflatoxin M1 (AFM1) of 42 marketed milk samples were determined with Enzyme-Linked Immunosorbent Assay (ELISA) to evaluate the status on the contamination of Aflatoxin M1. The evaluated ELISA performances of limit of detection (LOD) and the half maximal inhibitory concentration (IC 50 ) were 5 pg/mL (ppt) and 49 ppt, respectively. In all 42 samples, AFM1 appeared above the 5 ppt, with the average of 21 ppt and the range of up to 90 ppt. Only 3 (7%) of samples showed the level of contamination above the EU MRL (50 ppt). Although there was incidence of higher level of contamination compared with previous reports, the result of this study requires more intensive study to control of AFM1 in milk and infant foods.

Analysis of aflatoxin M1 and M2 in commercial dairy products using high-performance liquid chromatography with a fluorescence detector

“…According to previous studies, the milk sterilization method affects AFM 1 concentration in milk. AFM 1 concentrations in pasteurized milk have been shown to be higher than those of raw and UHT milk (Hwang, Hwang, Kim, & Oh, 2012;Kocasari et al, 2012;Zheng et al, 2012). In this study, pasteurized milk had higher mean values of AFM 1 and AFM 2 concentrations than UHT milk.…”

Influence of a non-dairy probiotic matrix based on wheat bran and soybean meal on lactic acid bacteria growth

“…During fermentation, most of the monosaccharides were used by LAB as carbon sources, so the monosaccharide content reduced markedly. Where enzymolysis also occurred during LAB fermentation, for example, it is reported that L. rhamnosus was able to produce a high beta-glucosidase 28,29 activity, which could further decompose macromolecule sugars in the matrix. Therefore, the polysaccharide content was further decreased, and the oligosaccharide content increased, supplied by the polysaccharides.…”

Immunochromatographic strip development for ultrasensitive analysis of aflatoxin M1

“…4 The risk of AFM1 in milk is a major concern due to the high daily consumption rates of milk worldwide. [5][6][7] The tolerance level for AFM1 in milk products has been set at 0.5 mg L À1 (Chinese National Standard GB2761). Immunoassay, is a sensitive, low-cost and fast detection technique that has been reported to detect AFM1.…”