Aflatoxins in Foods and Feedstuffs. Part 1. The Fluorescence Micro‐method of Aflatoxin Determination in solution

Abstract

The fluorescence micromethod of aflatoxins B,, B,, G, and G, determination in solution is described. The amount about 0.1 kg of aflatoxins B, and GI, and 0'01 pg of aflatoxins B, and G, in one spot after elution with methanol can be determined, with accuracy about 5%. The results of described fluorescence method are in agreement with results of spectrophotometric, fluorodensitometric and visual determinations. A very strong influence of light on results of aflatoxins B, and G, determination was stated. Photodecomposition of aflatoxins B, and G, to several photoproducts was observed.In our previous paper [I] we described the occurrence of aflatoxins ingrain crops and other products, their biological effect, methods of determination and detoxification.The methods of determination of aflatoxins are based mainly on their ability to light absorption and fluorescence emission. The quantitative analysis by fluorescence method can be effected by visual evaluation directly on the chromatoplates, or fluorodensitometrically. Because of its subjectivity the first method gives inaccurate results. The second requires an expensive and complex equipment including a self-recording fluorodensitometer and an integrator enabling the calculation of concentration of individual aflatoxins from the surface of cones. A good separation of spots is required besides. This is why an attempt was made to apply the measurements of fluorescence after the elution of spots from the silica gel with methanol [2].

Mycobiota, Mycotoxins, and Heavy Metals From Instant Noodles in Saudi Arabia

“…The developed plates were detected before and after spraying with the different reagents under short wave (254 nm) and long wave (356 nm) ultra violet irradiation. Mycotoxins were identified by comparison with appropriate reference standards after each of the following treatments: a-Aflatoxins: Aflatoxins B 1 and B 2 fluorescent green under long wave UV light (Chelkowski et al,1974).The TLC was developed In a saturated Chamber with chloroform : acetone (9:1, V/V), then aflatoxins spots were served under long wave ultraviolet light.…”

Mycotoxins in cereal grain. Part XI. Simple multidetection procedure for determination of 11 mycotoxins in cereals

“…Previous analytical papers on mycotoxins dealt with aflatoxins assay only [2,9,10,251. In this paper experiences of determination of several other mycotoxins are summarized.…”