AFLP-based fingerprinting of diverse chickpea genotypes: origin, history of chickpea

Talebi, Reza; Jelodar, Nadali Babaeian; Fayyaz, Farzad; Mardi, Mohsen

doi:10.1016/j.nbt.2009.06.757

Cited by 1 publication

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“…To overcome the limitations of reproducibility associated with RAPD, AFLP marker system was developed by selective amplifi cation of DNA fragments obtained by restriction enzyme digestion. AFLP markers have been used for genetic diversity estimation in cultivated chickpea and its wild relatives in order to discover the origin and history of chickpea (Nguyen et al 2004 ;Talebi et al 2008Talebi et al , 2009 ). However, the requirement of signifi cant technical skills, laboratory facilities, fi nancial resources and high quality genomic DNA for complete restriction, digestion and dominant inheritance has limited the use of AFLP markers.…”

Section: Molecular Markersmentioning

confidence: 99%

Advances in Chickpea Genomics

Gaur

Thudi

Srinivasan

et al. 2013

Legumes in the Omic Era

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Chickpea ( Cicer arietinum L.), the second largest consumed pulse crop of the world after common bean, is grown in over 50 countries and traded across 140 countries. After several decades of slow progress, the recent years have witnessed spectacular progress in development of genetic (mapping populations) and genomic resources (structural and functional molecular markers, integrated genetic map and mapping of genes/quantitative trait loci; QTLs) in this crop. QTLs associated with traits of interest including resistance against wilt, Ascochyta blight, Botrytis grey mould and rust; tolerance against salinity and drought and agronomic traits have been identifi ed and validated. A more than 30 genetic linkage maps available in this crop are useful resources for genetic analysis and marker assisted breeding. Genomic tools like bacterial artifi cial chromosome (BAC) libraries, expressed sequence tags (ESTs) and targeting induced local lesions in genome (TILLING) mutants have been developed in chickpea to facilitate the genome sequencing efforts in this crop. A major landmark in chickpea genomics has been the publication of 738 Mb draft whole genome sequence assembly of a kabuli variety, CDC Frontier. Now, chickpea is one of the most advanced grain legumes in terms of availability of genomic resources. Efforts have already begun on application of these genomics resources in chickpea improvement. This book chapter provides an update on the development of genetic and genomic resources for chickpea and their current and potential uses in chickpea improvement.

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Section: Molecular Markersmentioning

confidence: 99%