AFLP-Based Genetic Structure of Lithuanian Populations of Small Balsam (Impatiens parviflora DC.) in Relation to Habitat Characteristics

Jocienė, Lina; Stravinskaitė, Kristė; Krokaitė, Edvina; Janulionienė, Rasa; Rekašius, Tomas; Paulauskas, Algimantas; Marozas, Vitas; Kupčinskienė, Eugenija

doi:10.3390/f13081228

Cited by 3 publications

(12 citation statements)

References 72 publications

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“…Amplified fragment length polymorphism analysis was performed according to the protocols of Voss et al [84] with some modifications [96][97][98]. Restriction and ligation reactions were carried out for 2 h at 37 • C in a final volume of 11 µL containing 200 ng of DNA, 0.05 mg/mL BSA, 1X T4 DNA ligase buffer, 0.05 M NaCl (Invitrogen by Thermo Fisher Scientific, Vilnius, Lithuania), 5 U EcoRI and 1 U MseI of restriction enzymes (New England BioLabs, Ipswich, MA, USA), 67 U T4 DNA ligase, 0.45 pmol/µL EcoR I adapter (F: 5 CTCTGACTGCGTACC3 , R: 5 AATTGGTACGCAGTA3 ) and 4.5 pmol/µL MseI adapter (F: 5 GACGATGAGTCCTGAG3 , R: 5 CTACTCAGGACTCAT3 ) (Invitrogen by Thermo Fisher Scientific, Vilnius, Lithuania).…”

Section: Molecular Analysismentioning

confidence: 99%

The Molecular Evidence for Invasive Climber Echinocystis lobata (Michx.) Torr. & A. Gray in Eastern and Central Europe

Jocienė,

Krokaitė,

Rekašius

et al. 2023

Diversity

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The climbing cucurbit Echinocystis lobata, native to North America and alien to many European countries, was assessed for its genetic diversity and differentiation across its introduced range of populations by applying markers of amplified fragment length polymorphism (AFLP). Various tests, including an evaluation of the intrapopulation diversity, principal coordinate, and molecular variance analyses, showed that the Central and Eastern European populations differing in geography and arrival history are also distinct in the genetic parameters. Genetic diversity, defined as the percentage of polymorphic AFLP loci, ranged within 28–62% (on average 51%) at the regional scale (in Romanian, Baltic State, and Central Russian populations), and was very similar to this parameter at the local scale (on average 52% for Lithuanian populations). The differentiation was significant among the populations of the regions (Ф = 0.125, p = 0.001) and at the local scale (among the Lithuanian populations of the different river basins, Ф = 0.058, p = 0.010). The Bayesian results suggested the presence of three genetic clusters among the 29 sites, with populations from Romania, Latvia, Estonia, and the northern part of Lithuania comprising one prevailing cluster, populations from the Nemunas river basin of Lithuania comprising either the former mentioned cluster or the second cluster, and populations of Central Russia comprising the third genetic cluster. Overall, E. lobata in Europe has probably originated from multiple introductions. The intentional anthropogenic seed dispersal by marketing accompanied by hydrochory might have an impact on such a profile of genetic clusters.

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Section: Molecular Analysismentioning

confidence: 99%

The Molecular Evidence for Invasive Climber Echinocystis lobata (Michx.) Torr. & A. Gray in Eastern and Central Europe

Jocienė,

Krokaitė,

Rekašius

et al. 2023

Diversity

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“…For various plant species, including junipers, Ellenberg indicator values (EIV) of neighboring herbaceous plant species are widely used to evaluate environment in the sites without the need to perform physico-chemical measurements [ 73 , 74 , 75 , 76 , 77 ]. During evaluation of J. communis environments by EIV, the soil richness in nutrients (EIV-N) [ 35 ] showed low fertility for all habitats; it was lower than the middle value (5) and ranged in the interval of 2.7 to 4.4.…”

Section: Discussionmentioning

confidence: 99%

“…To relate ionomic data with other characteristics of J. communis , the genetic diversity of juniper populations was evaluated using ISSR markers [ 39 ]. For comparison of ionomic data with environmental parameters, undergrowth trees and shrubs, herbaceous species, and dwarf shrubs were recorded at each site following Braun-Blanquet [ 90 ] and the abundance of each species was evaluated using EIV [ 35 ] as described in detail previously [ 72 , 75 , 76 ].…”

Section: Methodsmentioning

confidence: 99%

Ionomic Parameters of Populations of Common Juniper (Juniperus communis L.) Depending on the Habitat Type

Jocienė

Krokaitė

Rekašius

et al. 2023

Plants

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For the study of the ionomic parameters of Juniperus communis needles, fourteen sites covering most of the territory of Lithuania and belonging to distinct habitats (coastal brown dunes covered with natural Scots pine forests (G), Juniperus communis scrubs (F), transition mires and quaking bogs (D), subcontinental moss Scots pine forests (G), and xero-thermophile fringes) were selected. Concentrations of macro-, micro-, and non-essential elements were analyzed in current-year needles, sampled in September. According to the concentrations of elements in J. communis needles, the differences between the most contrasting populations were as follows: up to 2-fold for Mg, N, K, Ca, and Zn; 2- to 7-fold for P, Na, Fe, Cu, Al, Cr, Ni, and Pb; and 26- to 31-fold for Mn and Cd. The concentrations of Cd, Cr, and Ni in needles of J. communis did not reach levels harmful for conifers. When compared to all other habitats (B, F, G, and E), the populations from transition mires and quaking bogs (D) had significantly lower concentrations of main nutritional elements N (12176 µg/g d. m.), P (1054 µg/g d. m.), and K (2916 µg/g d. m.). In Juniperus communis scrubs (F), a habitat protected by EUNIS, the concentration of K in the needles was highest, while Zn and Cu concentrations were the lowest. Principal component (PC) analyses using concentrations of 15 elements as variables for the discrimination of populations or habitats allowed authors to distinguish F and B habitats from the E habitat (PC1) and F and D habitats from the G habitat (PC2). Discriminating between populations, the most important variables were concentrations of P, N, Mg, Ca, Cu, and K. Discriminating between habitats, the important variables were concentrations of N and P.

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“…Disclosing possible effects of the river and its environment on nutritional state of the plant, Lithuanian populations of P. arundinacea were grouped in several ways: (1) three groups, according to the type of land cover and use class: artificial surfaces (ART), agricultural areas (AGR), forest, and semi-natural areas (FOR) [79]; (2) five groups, according to the present river state: high (H), good (G), moderate (MO), poor (P), bad (B) [78,79]; (3) three groups: populations of North-West (NW), Central (C) and South-East (SE) of Lithuania, based on N concentrations in the rivers neighboring agricultural areas during 1991-1996 [82]; (4) four groups, based on the river size: small (S, <100 km 2 ), medium sized (M, 100-1000 km 2 ), large (L, 1000-10,000 km 2 ), and extra-large (XL, >10,000 For GBS-SNP analysis using DArTSeq (https://www.diversityarrays.com/services /dartseq/, 15 May 2023), six populations of the Nemunas basin were selected: the upper part of the Nemunas River (Lithuanian part, LT3), the middle part of the Nemunas River (LT4), and the Atmata branch of the Nemunas Delta (LT6), as well as the population from the largest tributary of the Nemunas-Neris (LT5), and two populations from the rivers of the Nemunas tributary and the Merkys river basin (LT1 and LT2), previously studied by microsatellites [63]. Among the GBS-SNPs selected for analysis, all populations were near natural riverbeds, except for the LT1 population (regulated riverbed; Table S3).…”

Section: Environmental Variablesmentioning

confidence: 99%

“…(3) three groups: populations of North-West (NW), Central (C) and South-East (SE) of Lithuania, based on N concentrations in the rivers neighboring agricultural areas during 1991-1996 [82]; (4) four groups, based on the river size: small (S, <100 km 2 ), medium sized (M, 100-1000 km 2 ), large (L, 1000-10,000 km 2 ), and extra-large (XL, >10,000 km 2 ) rivers (EU, 2000); and (5) two groups, based on riverbed origin: natural and regulated [12] (Table S5).…”

Section: Environmental Variablesmentioning

confidence: 99%

Genetic Differentiation of Reed Canarygrass (Phalaris arundinacea L.) within Eastern Europe and Eurasia

Anderson,

Krokaitė-Kudakienė,

Jocienė

et al. 2024

Genes

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Worldwide molecular research of economically important Phalaris arundinacea (Poaceae) is mainly focused on the invasions of this species from Europe to North America. Until the present study, the genetic diversity of the P. arundinacea had not been studied across the Baltic countries. The objective of this research is to evaluate the diversity of Lithuanian populations of P. arundinacea at simple sequence repeat (SSR) loci comparatively among populations of the Baltic countries, Luxembourg, and the Russian Far East (Eurasian), evaluating differentiation between Lithuanian populations and ornamental accessions, and relating these with environmental features. For six selected Lithuanian river basin populations, GBS low density SNPs were used to determine genetic diversity. Bayesian analysis showed that Eurasian populations of Phalaris arundinacea consist of two gene clusters. Statistically significant genetic differentiation among European and Eurasian populations was documented. Lithuanian genotypes growing naturally along rivers are genetically distinct from cultivated ornamentals. GBS-SNPs divided the six selected Nemunas river basins into three distinct groups with one, two, or three rivers in separate groupings for genetic diversity. Genetic diversity is primarily within, rather than among, Lithuanian, eastern European, and Eurasian populations of P. arundinacea across the continent. Thus, restoration efforts would benefit from local population seed origination.

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AFLP-Based Genetic Structure of Lithuanian Populations of Small Balsam (Impatiens parviflora DC.) in Relation to Habitat Characteristics

Cited by 3 publications

References 72 publications

The Molecular Evidence for Invasive Climber Echinocystis lobata (Michx.) Torr. & A. Gray in Eastern and Central Europe

The Molecular Evidence for Invasive Climber Echinocystis lobata (Michx.) Torr. & A. Gray in Eastern and Central Europe

Ionomic Parameters of Populations of Common Juniper (Juniperus communis L.) Depending on the Habitat Type

Genetic Differentiation of Reed Canarygrass (Phalaris arundinacea L.) within Eastern Europe and Eurasia

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